Search Results

You are looking at 1 - 10 of 714 items for :

  • "high-performance liquid chromatography" x
  • All content x
Clear All

-electron capture detection (GC-ECD) [ 9 ], gas chromatography-mass spectrometry (GC-MS) [ 10 ], gas-liquid chromatography (GLC) [ 11 ], high-performance liquid chromatography (HPLC) [ 12–14 ], and ultra-high-performance liquid chromatography-quadrupole time of

Open access

.2 96.3–104 90/h [ 22 ] HF-LPME, hollow-fiber liquid-phase microextraction; HPLC–UV, high-performance liquid chromatography

Open access
Orvosi Hetilap
Authors: Lajos Markó, Gergő Attila Molnár, Zoltán Wagner, Tamás Kőszegi, Zoltán Matus, Márton Mohás, Mónika Kuzma, István András Szijártó, and István Wittmann

mtsai: Population prevalence of albuminuria in the Australian Diabetes, Obesity, and Lifestyle (AusDiab) study: immunonephelometry compared with high-performance liquid chromatography. Am. J. Kidney Dis., 2006, 47 , 604

Restricted access

Summary

A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N = 3) ranging from 1 × 10−8 to 1.5 × 10−4 mol L−1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

Restricted access

Summary

A high-performance liquid chromatography (HPLC) method has been developed for simultaneous determination of six alkaloids, i.e., (−)-(R)-platydesmin, noroxyhydrastinine, berberine, skimmianine, canthin-6-one, and pteleine in the herbal medicine of Phellodendron amurense Rupr. The optimal condition for extraction and separation was achieved with a linear mobile phase gradient prepared from 0.1% phosphoric acid and acetonitrile. The LODs and LOQs for the analytes ranged from 0.06 to 0.22 μg mL−1 and from 0.25 to 0.80 μg mL−1, respectively. The optimized method was applied to the determination of alkaloids in P. amurense Rupr. and was found to be efficient. This method can provide a scientific and technical platform to the manufacturers for setting up a quality control standard as well as to the public for quality and safety assurance of the proprietary traditional Chinese medicines.

Restricted access

glycosylated vitamin B 6 in wheat by high-performance liquid chromatography. Cereal Chem. , 72 , 217–223. Lorenz K. Analysis of free and glycosylated vitamin B6 in wheat by high-performance

Restricted access

Batey, I.L., Gupta, R.B., MacRitchie, F. 1991. Use of size-exclusion high-performance liquid chromatography in the study of wheat flour proteins: An improved chromatographic procedure. Cereal Chem. 68 :207

Restricted access
Acta Chromatographica
Authors: Chen Cheng, Nie Cun-Xi, Liang Jing, Wang Yong-Qiang, Liu Yan-Feng, Ge Wen-Xia, and Zhang Wen-Ju

developed report of the determination of gossypol carried out on animal plasma [ 9 – 11 ], tissue [ 11 , 12 ], and the cottonseed [ 8 , 13 ] and oil [ 14 ] was mostly based on high-performance liquid chromatography (HPLC) [ 11 , 13 ], high performance

Open access

validated to give reliable and reproducible data [ 7 ]. High performance liquid chromatography (HPLC) and HPLC-MS/MS are the widely used methods to quantify Esomeprazole in biological fluids [ 8–11 ]. As development of analytical method is evolving through

Open access

Summary

A novel liquid-phase microextraction (LPME) technique, based on a hollow fiber (HF), in conjunction with high-performance liquid chromatography, has been developed for analysis of melamine in milk products. Melamine was extracted directly from milk products by use of a hollow-fiber membrane filled with organic solvent. HFLPME conditions, for example pH, extraction solvent, temperature, stirring rate, and extraction time were optimized. The best extraction efficiency of melamine was achieved under the conditions: pH 9.5, 35 μL n-octanol as extraction solvent, temperature 55°C, stirring rate 300 rpm, and extraction time 30 min. The HF-LPME technique resulted in a preconcentration ratio of 29-fold. Baseline chromatographic separation of melamine was achieved on a C18 column with 96:4 (v/v) 0.02 mol L−1 ammonium sulfate-methanol as isocratic mobile phase. The linearity of the method ranged from 1.0 to 100.0 μg mL−1, correlation coefficient 0.9994. The limit of detection by use of HF-LPME was 0.021 μg mL−1 at a signal-to-noise ratio of 3. The optimized HF-LPME technique was successfully applied to the analysis of melamine in milk products collected from different commodity manufacturing units.

Restricted access