Hypothermia has been shown to induce significant changes in responsiveness of vascular smooth muscle cells to various drugs ( 6 , 7 ). Most of the previous studies examining the effect of hypothermia on smooth
Temperature can influence endothelial and vascular smooth muscle cell function and alter the reactivity of the vessels. Moderate hypothermia, cooling to 25–31 °C, has been shown to have a variable influence on the
Authors:Ágnes Ferencz, Renáta Juhász, Monica Butnariu, Aranka Deér, Ilona Varga and J. Nemcsók
Heat shock proteins are chaperones that play a pivotal role in controling multiple regulatory pathways such as stress defense, hormone signaling, cell cycle control, cell proliferation and differentiation, and apoptosis. In this study, the expression patterns of four well-known heat shock genes (hsp70, hsc70-1, hsc70-2 and hsp90α) were characterized in the skin, spleen and blood cells of the common carp, under unstressed conditions and after Cd2+ treatment or hypothermia. The examined genes were expressed in a tissue-specific manner: hsc70-2 was expressed constitutively, and was at best only slightly inducible; hsp90α exhibited a high basic expression in all three tissues, whereas hsc70-1 did so only in the blood cells, the expression of hsp70 proved to be below the level of detection in unstressed fish. Cold shock induced the expression of hsp genes in the spleen (hsp90α) and blood cells (hsp70, hsc70-1 and hsp90α), while Cd2+ treatment has no effect on the expression pattern. The highest inducibilities were detected in the skin: for hsp70 an induction of at least 20-fold after cadmium exposure, for hsc70-1 of at least 30-fold and for hsp90α of 3-fold after hypothermia.