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Abstract

Campylobacter spp. are fastidious microorganisms, and their detection by culture depends on the freshness of the stool sample and the skills of the laboratory staff. To improve laboratory diagnosis, assays for the detection of specific antigens have been developed. Here, we evaluated two assays for the detection of Campylobacter spp.-specific antigens, i.e., one immunochromatographic test and one enzyme-linked immunosorbent assay (EIA), in 38 frozen Campylobacter spp.-positive specimens and prospectively in 533 fresh stool samples with a conventional enzyme immunoassay (EIA) and culture.

Both assays were positive for 36 samples with Campylobacter jejuni and one with Campylobacter coli among 38 Campylobacter spp.-positive frozen samples. One Campylobacter lari-positive sample was identified by the immunochromatographic assay (ICA) only. In a prospective study performed within the course of routine microbiology, both assays were positive for 24/25 C. jejuni culture-positive samples (positive percent agreement, 96.0% [95% CI: 78.9–100%]). ICA and EIA also were positive for 14 and 10 culture-negative samples, respectively (negative percent agreement: ICA, 97.2% [95% CI: 95.4–98.4%]; EIA, 98.0% [95% CI: 96.4–99.0%]). In conclusion, the high agreement between both antigen-detection assays and culture indicates that both assays may be initially performed followed by culture only upon a positive test result.

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Acta Veterinaria Hungarica
Authors: Orsolya Erdősi, Katalin Szakmár, Zsuzsanna Szili, Géza Szita, Sándor Bernáth, József Sövényi and Péter Laczay

immunochromatographic assay . Int. J. Food Microbiol. 142 , 256 – 259 . Kemmeren , J. M. , Mangen , M. J. , van Duynhoven , Y. T. and Havelaar , A. H. ( 2005 ): Priority Setting of Foodborne

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nanopatricle-labeled secondary antibodies to improve the sensitivity of an immunochromatographic assay for aflatoxin . Microchim Acta 181 : 1939 – 1946 . Urusov , A.E. , Petrakova , A

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staff [ 2 , 3 ]. In addition, rapid diagnostic antigen tests (RDTs) are recommended by current guidelines [ 1 , 4 ]. RDTs are lateral flow immunochromatographic assays that offer an easy handling and results within a few minutes, but they are less

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and yeasts; lower panel, colorless oocysts that have been associated with resolving infection. (B) Immunochromatographic assay for detecting Cryptosporidium oocysts in stool samples, (C) ELISA assay for the detection of Cryptosporidium antigen in

Open access
European Journal of Microbiology and Immunology
Authors: Kouassi N’Guessan, Timothée Ouassa, Anna S. Dean, Riccardo Alagna, Guy Damien Adagra, Valeri Ibode, Daniela M. Cirillo and Jacquemin Kouakou

until a positive signal developed. Solid media were incubated at 37 °C for 8 weeks and were inspected weekly or until mycobacterial colonies were seen. Cultures were considered positive only after detection of MTBC by the immunochromatographic assay, SD

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2020 ; 2 : 118 – 25 . 10.1089/mdr.2019.0086 33. Sağıroğlu P , Hasdemir U , Altınkanat Gelmez G , Aksu B , Karatuna O , Söyletir G . Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like

Open access