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Acta Veterinaria Hungarica
Authors:
Péter Vajdovich
,
Zsófia Koltai
,
Valéria Dékay
,
Krisztina Kungl
, and
Andrea Harnos

Permeability glycoprotein (P-glycoprotein, Pgp) immunohistochemistry (IHC) was evaluated in dogs with multicentric lymphoma treated with cyclophosphamide– doxorubicin–vincristine–prednisolone with or without L-Asparaginase. Lymph nodes of 33 untreated dogs were immunophenotyped: Ki67% and Pgp analyses (with anti-Pgp, monoclonal mouse C494 clone) were performed. Pgp positivity rate and intensity were determined microscopically (by manual counting done by two blinded authors in two parallel specimens). The median overall survival time (OST) was 333 days and the relapse-free period (RFP) 134 days. Pgp expressions were positive in 18 out of 33 (54.5%) of tumour cells. T-cell types stained more intensively. Lower OST and RFP were found with Pgp positivity ≥ 35% (OST: 240 days, RFP: 95 days) compared to Pgp positivity < 35% (OST: 428 days, RFP: 232 days). Intensive staining was associated with a lower OST and RFP (240 and 103 days, respectively) than weak staining (428 and 221 days, respectively). Death due to adverse drug reactions was best predicted at Pgp positivity ≤ 6.5% (sensitivity/specificity: 0.55/0.81) and ≤ 123 days (sensitivity/ specificity: 0.55/0.86). Pgp evaluation by IHC can have prognostic value with a properly established Pgp% positivity cut-off value in dogs treated with Pgp substrate drugs.

Open access
European Journal of Microbiology and Immunology
Authors:
Cosme Alvarado-Esquivel
,
Luis Francisco Sánchez-Anguiano
,
Alejandra Mendoza-Larios
,
Jesús Hernández-Tinoco
,
José Francisco Pérez-Ochoa
,
Elizabeth Irasema Antuna-Salcido
,
Elizabeth Rábago-Sánchez
, and
Oliver Liesenfeld

The presence of tissue cysts of Toxoplasma gondii has only poorly been investigated in autopsy series. We determined the presence of T. gondii cysts in a series of 51 autopsies in a public hospital using immunohistochemistry of brain and heart tissues. The association of tissue cysts with the general characteristics of the autopsy cases was also investigated.

Of the 51 cases studied, five (9.8%) were positive by immunohistochemistry for T. gondii cysts in the brain. None of the heart specimens was positive for T. gondii cysts. The presence of T. gondii cysts in brains did not vary with age, sex, birthplace, residence, education, occupation, or the presence of pathology in the brain. In contrast, multivariate analysis showed that the presence of T. gondii cysts was associated with undernourishment (OR = 33.90; 95% CI: 2.82–406.32; P = 0.005).

We demonstrated cerebral T. gondii cysts in an autopsy series in Durango City, Mexico. Results suggest that T. gondii can be more readily found in brain than in heart of infected individuals. This is the first report of an association between the presence of T. gondii in brains and undernourishment.

Open access
Acta Veterinaria Hungarica
Authors:
Csaba Jakab
,
Judit Halász
,
András Kiss
,
Zsuzsa Schaff
,
Attila Szász
,
Miklós Rusvai
,
Zsolt Abonyi Tóth
, and
Janina Kulka

In our recent investigation, angiogenesis was evaluated and quantified by immunohistochemical evaluation of microvessel density (MVD) using claudin-5 (CLDN-5) as a marker for vascular endothelium in 67 canine mammary gland tumours. Computer image analysis was used to measure the intratumoural MVD. Higher intratumoural MVD was detected in malignant simple neoplasms compared with benign tumours. Furthermore, the results of MVD were correlated with histological grade, higher grades being accompanied by higher MVD. In simple adenomas and grade I tubular-tubulopapillary simple carcinomas the intratumoural microvessels were wide and regular in shape with evident erythrocytes in their lumen. In grade III solid carcinomas the microvessels were smaller, less regular and had irregular shape, often without a distinct lumen, and isolated endothelial cells were frequently present. In the complex carcinomas MVD was low and the intratumoural microvessels were mostly irregular in shape without a distinct lumen. The evaluation of MVD by CLDN-5 immunohistochemistry may give useful additional information on the angiogenic potential of breast cancers in dogs.

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, followed by Sasso broilers. Immunohistochemistry ND antigen was detected in the different tissues with strong positive peroxidase reaction involving the trachea ( Fig. 4a ), lung tissue ( Fig. 4b ), proventriculus ( Fig. 4c ), pancreas ( Fig. 4d ), liver

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Acta Veterinaria Hungarica
Authors:
Federica Giorda
,
Giovanni Di Guardo
,
Katia Varello
,
Alessandra Pautasso
,
Eva Sierra
,
Maria Domenica Pintore
,
Carla Grattarola
,
Erika Molica Colella
,
Enrica Berio
,
Maria Goria
,
Elena Bozzetta
,
Cristina Casalone
, and
Barbara Iulini

chain reaction (RT-PCR) followed by sequencing the PCR product is a specific and sensitive diagnostic method; however, histology and immunohistochemistry (IHC) should be performed to confirm morbillivirus infection (MI) and to obtain information on the

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Acta Veterinaria Hungarica
Authors:
Thiago Henrique M. Vargas
,
Camila N. Barra
,
Lidia H. Pulz
,
Greice C. Huete
,
Karine G. Cadrobbi
,
Adriana Tomoko Nishiya
,
Silvia Regina Kleeb
,
José Guilherme Xavier
,
José Luiz Catão-Dias
, and
Ricardo F. Strefezzi

involving the use of animals were approved by the Animal Ethics Committee of FZEA-USP (#1005140116/2016) and owners provided informed client consent for the research. Four-µm histological sections were placed onto silanised slides for immunohistochemistry

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„Dum spiro spero”: a SARS-CoV-2-fertőzés klinikopatológiája 26 eset kapcsán

“Dum spiro spero”: clinicopathologic characteristics of SARS-CoV-2 infection

Orvosi Hetilap
Authors:
Tamás Zombori
,
Levente Kuthi
,
Tibor Hortobágyi
,
Erika Csörgő
,
János Árgyelán
,
Lajos Kocsis
,
István Sejben
,
László Kaizer
,
Bence Radics
,
Anita Sejben
,
Tamás Pancsa
,
Gergely Róbert Nyári
,
Bence Baráth
,
Gábor Cserni
,
Béla Iványi
, and
László Tiszlavicz

-embedded specimens. JCI Insight 2020; 5: e139042. 36 Massoth LR, Desai N, Szabolcs A, et al. Comparison of RNA in situ hybridization and immunohistochemistry

Open access

Background

Fragile histidine triad (FHIT) is a tumor suppressor gene that is commonly inactivated in human tumors. Interestingly, the normal pattern of FHIT expression is largely unknown.

Aim

This study is aimed to characterize the expression of FHIT protein in normal human tissues.

Materials and methods

A total of 119 normal human tissue specimens were analyzed for the FHIT expression using immunohistochemistry technique. The inclusion criteria included: normal/inflammatory tissue with no evidence of cellular atypia.

Results

All studied specimens were stained positively with FHIT and showed either nuclear or cytoplasmic expression. Interestingly, the pattern of FHIT staining was similar among different specimens from each organ. FHIT is located predominantly in the nucleus, although cytoplasmic staining is also present in some cell types. Oral squamous epithelium, breast ductal epithelium, squamous and tubal metaplastic epithelium of the uterine cervix, esophageal squamous epithelium, salivary glands, and bronchial epithelia all strongly expressed the nuclear protein. In connective tissue, FHIT has shown strong cytoplasmic expression in histocytes including macrophages and dendritic cells, fibroblasts, and myofibroblasts.

Conclusion

Documentation of the pattern of FHIT expression in normal tissues will contribute to our understanding of the normal function of this protein and to interpretation of potentially altered FHIT expression in human tumors.

Open access
Acta Veterinaria Hungarica
Authors:
Carmen Solcan
,
Geta Pavel
,
Viorel Floristean
,
Ioan Chiriac
,
Bogdan Şlencu
, and
Gheorghe Solcan

The immunotoxic effect of ochratoxin A (OTA) on the intestinal mucosa-associated lymphoid tissue and its cytotoxic action on the intestinal epithelium were studied in broiler chickens experimentally treated with the toxin. From the 7th day of life, 80 male broiler chickens (Ross 308) were randomly divided into four groups of 20 birds each. The three experimental groups (E1-3) were treated with OTA for 28 days (E1: 50 μg/kg body weight [bw]/day; E2: 20 μg/kg bw/day; E3: 1 μg/kg bw/day) and the fourth group served as control. Histological examination of the intestinal mucosa and immunohistochemical staining for identification of CD4+, CD8+, TCR1 and TCR2 lymphocytes in the duodenum, jejunum and ileocaecal junction were performed, and CD4+/CD8+ and TCR1/TCR2 ratios were calculated. OTA toxicity resulted in decreased body weight gain, poorer feed conversion ratio, lower leukocyte and lymphocyte count, and altered intestinal mucosa architecture. After 14 days of exposure to OTA, immunohistochemistry showed a significant reduction of the lymphocyte population in the intestinal epithelium and the lamina propria. After 28 days of exposure, an increase in the CD4+ and CD8+ values in both the duodenum and jejunum of chickens in Groups E1 and E2 was observed, but the TCR1 and TCR2 lymphocyte counts showed a significant reduction. No significant changes were observed in Group E3. The results indicate that OTA induced a decrease in leukocyte and lymphocyte counts and was cytotoxic to the intestinal epithelium and the mucosa-associated lymphoid tissue, altering the intestinal barrier and increasing susceptibility to various associated diseases.

Open access

The aim of experiments was to characterize the neurons of the autonomic chain that innervates the nipple and the mammary gland of lactating rats using retrograde transynaptic virus labeling and neurotransmitter and neuropeptide immunohistochemistry. Two days after injection of green fluorescence protein labeled virus in two nipples and underlying mammary glands, labeling was observed in the ipsilateral paravertebral sympathetic trunk and the lateral horn. Three days after inoculation the labeling appeared in the brain stem and the hypothalamic paraventricular nucleus. Above the spinal cord the labeling was bilateral. A subpopulation of virus labeled cells in the paraventricular nuclei synthesized oxytocin. Labeled neurons in the lateral horn showed cholinergic immunoreactivity. These cholinergic neurons innervated the paravertebral ganglia where the virus labeled neurons were partially noradrenergic. The noradrenergic fibers in the mammary gland innervate the smooth muscle wall of vessels, but not the mammary gland in rats. The neurons in the lateral horn receive afferents from the brain stem, and paraventricular nucleus and these afferents are noradrenergic and oxytocinergic. New findings in our work: Some oxytocinergic fibers may descend to the neurons of the lateral horn which innervate noradrenergic neurons in the paravertebral sympathetic trunk, and in turn these noradrenergic neurons reach the vessels of the mammary gland.

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