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. Bergman , P. J. , Ogilvie , G. K. and Powers , B. E. ( 1996 ): Monoclonal antibody C219 immunohistochemistry against P-glycoprotein: sequential analysis and predictive ability in dogs with lymphoma . J. Vet. Intern. Med. 10 , 354 – 359

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Acta Veterinaria Hungarica
Authors: Csaba Jakab, Judit Halász, András Kiss, Zsuzsa Schaff, Attila Szász, Miklós Rusvai, Zsolt Abonyi Tóth, and Janina Kulka

Kohlberger, P. D., Obermair, A., Sliutz, G., Koelbl, H., Breitenecker, G., Gitsch, G. and Kainz, C. (1996): Quantitative immunohistochemistry of factor VIII-related antigen in breast carcinoma. Am. J. Clin. Pathol. 105 , 705

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European Journal of Microbiology and Immunology
Authors: Cosme Alvarado-Esquivel, Luis Francisco Sánchez-Anguiano, Alejandra Mendoza-Larios, Jesús Hernández-Tinoco, José Francisco Pérez-Ochoa, Elizabeth Irasema Antuna-Salcido, Elizabeth Rábago-Sánchez, and Oliver Liesenfeld

The presence of tissue cysts of Toxoplasma gondii has only poorly been investigated in autopsy series. We determined the presence of T. gondii cysts in a series of 51 autopsies in a public hospital using immunohistochemistry of brain and heart tissues. The association of tissue cysts with the general characteristics of the autopsy cases was also investigated.

Of the 51 cases studied, five (9.8%) were positive by immunohistochemistry for T. gondii cysts in the brain. None of the heart specimens was positive for T. gondii cysts. The presence of T. gondii cysts in brains did not vary with age, sex, birthplace, residence, education, occupation, or the presence of pathology in the brain. In contrast, multivariate analysis showed that the presence of T. gondii cysts was associated with undernourishment (OR = 33.90; 95% CI: 2.82–406.32; P = 0.005).

We demonstrated cerebral T. gondii cysts in an autopsy series in Durango City, Mexico. Results suggest that T. gondii can be more readily found in brain than in heart of infected individuals. This is the first report of an association between the presence of T. gondii in brains and undernourishment.

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Acta Veterinaria Hungarica
Authors: Csaba Jakab, Attila Szász, Janina Kulka, Ferenc Baska, Miklós Rusvai, Péter Gálfi, and Tibor Németh

, A., Schaff, Zs., Szász, A. M., Rusvai, M., Abonyi, T. Zs. and Kulka, J. (2008): Evaluation of microvessel density (MVD) in canine mammary tumours by quantitative immunohistochemistry of the claudin-5 molecule. Acta Vet. Hung. 56 , 495

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, followed by Sasso broilers. Immunohistochemistry ND antigen was detected in the different tissues with strong positive peroxidase reaction involving the trachea ( Fig. 4a ), lung tissue ( Fig. 4b ), proventriculus ( Fig. 4c ), pancreas ( Fig. 4d ), liver

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Acta Veterinaria Hungarica
Authors: Federica Giorda, Giovanni Di Guardo, Katia Varello, Alessandra Pautasso, Eva Sierra, Maria Domenica Pintore, Carla Grattarola, Erika Molica Colella, Enrica Berio, Maria Goria, Elena Bozzetta, Cristina Casalone, and Barbara Iulini

chain reaction (RT-PCR) followed by sequencing the PCR product is a specific and sensitive diagnostic method; however, histology and immunohistochemistry (IHC) should be performed to confirm morbillivirus infection (MI) and to obtain information on the

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Rimstad, E. and Evensen, O. 1993: The identification of equid herpesvirus 1 in paraffinembedded tissues from aborted fetuses by polymerase chain reaction and immunohistochemistry. J. Vet. Diagn. Invest. 5 , 174-183. The

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Acta Veterinaria Hungarica
Authors: Thiago Henrique M. Vargas, Camila N. Barra, Lidia H. Pulz, Greice C. Huete, Karine G. Cadrobbi, Adriana Tomoko Nishiya, Silvia Regina Kleeb, José Guilherme Xavier, José Luiz Catão-Dias, and Ricardo F. Strefezzi

involving the use of animals were approved by the Animal Ethics Committee of FZEA-USP (#1005140116/2016) and owners provided informed client consent for the research. Four-µm histological sections were placed onto silanised slides for immunohistochemistry

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The aim of experiments was to characterize the neurons of the autonomic chain that innervates the nipple and the mammary gland of lactating rats using retrograde transynaptic virus labeling and neurotransmitter and neuropeptide immunohistochemistry. Two days after injection of green fluorescence protein labeled virus in two nipples and underlying mammary glands, labeling was observed in the ipsilateral paravertebral sympathetic trunk and the lateral horn. Three days after inoculation the labeling appeared in the brain stem and the hypothalamic paraventricular nucleus. Above the spinal cord the labeling was bilateral. A subpopulation of virus labeled cells in the paraventricular nuclei synthesized oxytocin. Labeled neurons in the lateral horn showed cholinergic immunoreactivity. These cholinergic neurons innervated the paravertebral ganglia where the virus labeled neurons were partially noradrenergic. The noradrenergic fibers in the mammary gland innervate the smooth muscle wall of vessels, but not the mammary gland in rats. The neurons in the lateral horn receive afferents from the brain stem, and paraventricular nucleus and these afferents are noradrenergic and oxytocinergic. New findings in our work: Some oxytocinergic fibers may descend to the neurons of the lateral horn which innervate noradrenergic neurons in the paravertebral sympathetic trunk, and in turn these noradrenergic neurons reach the vessels of the mammary gland.

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„Dum spiro spero”: a SARS-CoV-2-fertőzés klinikopatológiája 26 eset kapcsán

“Dum spiro spero”: clinicopathologic characteristics of SARS-CoV-2 infection

Orvosi Hetilap
Authors: Tamás Zombori, Levente Kuthi, Tibor Hortobágyi, Erika Csörgő, János Árgyelán, Lajos Kocsis, István Sejben, László Kaizer, Bence Radics, Anita Sejben, Tamás Pancsa, Gergely Róbert Nyári, Bence Baráth, Gábor Cserni, Béla Iványi, and László Tiszlavicz

-embedded specimens. JCI Insight 2020; 5: e139042. 36 Massoth LR, Desai N, Szabolcs A, et al. Comparison of RNA in situ hybridization and immunohistochemistry

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