The quantitative analysis of antibiotics in different matrices is of increasing importance in in vitro studies of food-drug interactions in the drug-development process. Our objective was to develop a new, rapid and simple solid-phase extraction method without protein precipitation followed by high-performance liquid chromatography coupled with mass spectrometry (HPLC-MS) for analysis of ciprofloxacin in low- and high-fat milk. The HPLC-MS method (a single-quadrupole mass spectrometer equipped with an electrospray ion source was used as detector) was validated using a quinolone derivative, aripiprazole, as internal standard. A C8 column and gradient elution with 0.02 m ammonium acetate solution (pH 2.5) and acetonitrile mixtures (flow rate 0.5 mL min−1) were used for HPLC separation. Various modes of detection (diode-array detection and MS detection in scanning and selected-ion monitoring modes) were compared for selectivity and sensitivity of quantitative analysis of ciprofloxacin. Use of selected- ion monitoring resulted in a 1600:1 signal-to-noise ratio which can be successfully applied to milk matrices resulting in a lower limit of detection of 0.4 ng mL−1 and a lower limit of quantification of 4.0 ng mL−1. Recovery was 99.06 ± 0.15 and 98.57 ± 0.14% for low- and high-fat milk, respectively. The method enables analysis of the free, biologically available, amount of ciprofloxacin in in vitro simulation of milk-ciprofloxacin interaction studies.
Population prevalence of albuminuria in the Australian Diabetes, Obesity, and Lifestyle (AusDiab) study: immunonephelometry compared with high-performance liquidchromatography. Am. J. Kidney Dis., 2006,
Batey, I.L., Gupta, R.B., MacRitchie, F. 1991. Use of size-exclusion high-performance liquidchromatography in the study of wheat flour proteins: An improved chromatographic procedure. Cereal Chem. 68 :207
for determining malathion in different matrices are known. Thus, for example, the determination of malathion and its residues can be performed using gas–liquidchromatography (GLC) [ 3 ]. More than 30 years ago, malathion in pesticide formulations was
necessary to establish an analytical method to monitor the concentration of hapepunine.
To the best of our knowledge, the pharmacokinetics of hapepunine had not been reported. In this paper, an ultra-performance liquidchromatography–tandem mass
developed report of the determination of gossypol carried out on animal plasma [ 9 – 11 ], tissue [ 11 , 12 ], and the cottonseed [ 8 , 13 ] and oil [ 14 ] was mostly based on high-performance liquidchromatography (HPLC) [ 11 , 13 ], high performance
published. So, for example, the most commonly used are chromatographic methods, such as gas chromatography (GC) [ 5–8 ] liquidchromatography (LC) [ 9 , 10 ], high-performance liquidchromatography (HPLC) with UV-detection [ 11 , 12 ] and gas-liquid