Authors:M. Hudcovicová, V. Šudyová, S. Šliková, E. Gregová, J. Kraic, F. Ordon, D. Mihálik, V. Horevaj, and Z. Šramková
Gupta, S. K., Charpe, A., Koul, S., Prabhu, K. V., Haq, Q. M. R. (2005): Development and validation of molecular markers linked to an
-derived leaf rustresistance gene,
, for marker-assistedselection in bread wheat
Authors:I.N. Leonova, A.I. Stasyuk, E.S. Skolotneva, and E.A. Salina
Miedaner , T. , Korzun , V.
2012 . Marker-assistedselection for disease resistance in wheat and barley breeding . Phytopathol. 102 : 560 – 566 .
Morgounov , A
Submergence was a major abiotic stress in rice growing areas, particularly in the rainfed low land ecosystem of eastern India. Tolerance of complete submergence was recognized in some landraces and can be conferred by the Sub1 locus in chromosome 9. Farmers’ adaptation to these landraces and previously developed submergence tolerance genotypes was poor due to either their low yield potential and/or poor grain quality. The purpose of this study was introgressing Sub1 locus from tolerant genotype to high yielding susceptible cultivars with an aim of developing high yielding submergence tolerant genotypes with slender grain preferred by local consumer. During this study fifty lines were selected on the basis of higher yield potential from an advance generation (F4) of a cross between a submergence tolerant, natural mutant (P-1) and submergence susceptible rice cultivar (IR-36). Three already established SSR Primer pairs (RM-265, RM-219 and RM-464A), linked with submergence tolerance were employed to detect polymorphism among the two parents. Only RM-219 showed polymorphism among the parents in 2% agarose gel and employed for screening advance rice lines. Seven lines showed similar banding pattern with tolerant parent and screened for their submergence tolerance ability in the field in controlled and submerged conditions along with the two parents. Out of seven, six lines performed well in submerged condition. Finally on the basis of yield performance as well as slenderness of grain two genotypes were selected which will be very useful source for development of additional submergence tolerant high yielding mega varieties with preferred grain type.
Authors:R. Singh, P. Hucl, M. Båga, and R. Chibbar
Pre-harvest sprouting (PHS) in bread wheat (Triticum aestivum L.) is one of the major abiotic constraints influencing production of high quality grain. Selection for pre-harvest sprouting (PHS) resistance in bread wheat (Triticum aestivum L.) in early generations is difficult because it is expressed as a quantitatively inherited trait and subject to environmental effects. The objectives of this study were to validate a major quantitative trait locus (QTL) for PHS resistance on chromosome 4A in bread wheat and to isolate near-isogenic lines for this QTL using marker-assisted selection. A total of 60 Canadian wheat cultivars and experimental lines were screened with three SSR markers in a QTL region for PHS resistance. The SSR markers DuPw004, barc170 and wmc650 explained 67%, 75% and 60% of total variation in germination (%), respectively, among different wheat genotypes. Marker assisted back crossing with DuPw004 reduced the population size in BC1F1 and BC2F1 generation by 41% and 59%, respectively. A survey of pedigrees of different genotypes revealed that the parental line RL4137 is a major source of increased PHS resistance in a number of western Canadian wheat cultivars. Microsatellite markers (DuPw004, barc170 and wmc650) will be useful for plant breeders to pyramid QTL from different PHS resistance sources.