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Acta Biologica Hungarica
Authors: Szabolcs Tamás Nagy, Balázs Kakasi, László Pál, Máté Havasi, Miklós Bercsényi, and Ferenc Husvéth

. Hallap , T. , Nagy , S., Jaakma, Ü. , Johannisson , A. , Rodriguez-Martinez , H. ( 2005 ) Mitochondrial activity of frozen-thawed spermatozoa assessed by MitoTracker Deep Red 633 . Theriogenology 63 , 2311 – 2322 . 13

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Abstract  

It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells.

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Acta Veterinaria Hungarica
Authors: Caner Öztürk, Şükrü Güngör, Mehmet Bozkurt Ataman, Mustafa Numan Bucak, Nuri Başpinar, Pınar Ili, and Muhammed Enes Inanç

The present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 °C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 °C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 ± 8.21%), CASA motility (12.2 ± 5.69%) and progressive motility (3.52 ± 2.13%), compared with the controls (43 ± 2.73%, 55.4 ± 6.78% and 33.48 ± 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 ± 3.99% and 44.1 ± 2.18%) compared with the control (13 ± 8.15 and 31.7 ± 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.

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. Martin 2003 Defective hepatic mitochondrial respiratory chain in patients with nonalcoholic steatohepatitis Hepatology 38 999 – 1007 . [46]. K. F. Petersen S. Dufour D. Befroy 2004 Impaired mitochondrial activity in the insulin

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, J. , Leonce , S. , Jouannet , P. and Ronot , X. ( 1993 ): Flow cytometric sorting of living, highly motile human spermatozoa based on evaluation of their mitochondrial activity . J. Histochem. Cytochem. 41 , 1247 – 1251

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, dependent on mitochondrial activity. Biochem Biophys Res Commun 253 , 407-414 (1998). A region of the cellobiohydrolase I promoter from the filamentous fungus Trichoderma reesei mediates glucose repression in Saccharomyces

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Physiology International
Authors: NG Krylova, TA Kulahava, VT Cheschevik, IK Dremza, GN Semenkova, and IB Zavodnik

0 , and coenzyme Q 1 , but not coenzyme Q 10 , partially restored mitochondrial activity under conditions of impaired Complex I function ( 23 , 37 ). In our experiments, addition of amytal (10 µM), Complex I inhibitor, caused significant impairment

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Physiology International
Authors: HI Korkmaz, NE Hahn, KM Jansen, RJP Musters, J van Bezu, WN van Wieringen, PPM van Zuijlen, MMW Ulrich, HWM Niessen, and PAJ Krijnen

, Duan C , Zheng J : Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity . Exp. Ther. Med. 12 , 2247

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, Mercier J : Training-induced improvement in lipid oxidation in type 2 diabetes mellitus is related to alterations in muscle mitochondrial activity. Effect of endurance training in type 2 diabetes . Diabetes Metab

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mitochondrial activities are modified by anabolic-androgenic steroids . Med Sci Sports Exerc 31 , 243 – 250 ( 1999 ) 10.1097/00005768-199902000-00007 25. Dalton TP

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