Authors:L.R. Vemireddy, N. Ranjithkumar, A. Vipparla, M. Surapaneni, G. Choudhary, K.V. Sudhakarrao, and E.A. Siddiq
India bred high yielding rice varieties have enriched to a great extent the global rice germplasm since the mid-sixties. Systematic research efforts for development of cultivar-specific DNA fingerprints of major Indian rice cultivars, however, have not received due attention. The present investigation was aimed at development of DNA fingerprints for 90 high yielding rice varieties using hypervariable microsatellite (hvRM) markers. A panel of eight markers, viz. RM11313, RM13584, RM15004, RM5844, RM22250, RM22565, RM24260 and RM8207 was chosen from 52 polymorphic markers based on their highly polymorphic nature, SSR repeat type and number and ability to distinguish genotypes, in order to develop DNA fingerprints of 90 varieties. The remaining high polymorphic hvRM markers could be of immense value in future to distinguish new cultivars, in case they could not be distinguished by the 8 marker panel. Four of the 8 markers, viz. RM22250, RM13584, RM24260 and RM5844 were located in expressed genes and could be of value in DUS (Distinctness, Uniformity and Stability) testing. Thus we suggested, that this set of 8 loci could be used as standard for DNA fingerprinting of Indian rice cultivars.
Authors:Nóra Faragó, Ágnes Zvara, Z. Varga, P. Ferdinandy, and L. Puskás
Yanaihara, N., Caplen, N., Bowman, E., Seike, M., Kumanoto, K., Yi, M., Stephens, R. M., Okamoto, A., Yokota, J., Tanaka, T. (2006) Unique microRNA molecularprofiles in lung cancer diagnosis and prognosis. Cancer Cell 9 , 189
Authors:Csaba Jakab, Judit Halász, Attila Szász, Enkhjargal Batmunkh, András Kiss, Zsuzsa Schaff, Miklós Rusvai, Péter Gálfi, and Janina Kulka
, J., Bubendorf, L., Kallioniemi, A., Bärlund, M., Schraml, P., Leighton, S., Torhorst, J., Mihatsch, M. J., Sauter, G. and Kallioniemi, O. P. (1998): Tissue microarrays for high-throughput molecularprofiling of tumor specimens. Nat. Med.
Montero-Conde, C., Martin-Campos, J., Lerma, E. és mtsai: Molecularprofiling related to poor prognosis in thyroid carcinoma. Combining gene expression data and biological information. Oncogene, 2008, 27 , 1554
Authors:Tapas Mitra, G. Sailakshmi, A. Gnanamani, and A. B. Mandal
subjected to SDS-PAGE analysis to assess the purity, type, and molecularprofile. In brief, electrophoresis was carried out using 8% polyacrylamide gel. Followed by electrophoresis, gel was stained with coomassie blue and destained with the mixture of
Authors:Gábor Rubovszky, Nóra Udvarhelyi, Zsolt Horváth, István Láng, and Miklós Kásler
Tan , DS , Marchio , C , Jones , RL et al. 2008 Triple negative breast cancer: molecularprofiling and prognostic impact in adjuvant anthracyclin-treated patients Breast Cancer Res Treat 111 27 – 44 .