Authors:Ildikó Bódi, Nándor Nagy, Lídia Sinka, Botond-Zoltán Igyártó, and Imre Oláh
. and Ginsberg, M. H. (1991): Monoclonalantibodies to ligand-occupied conformers of integrin alpha IIb beta 3 (glycoprotein IIb–IIIa) alter receptor affinity, specificity, and function. J. Biol. Chem.
Authors:I. Abdel-Ghany, A. El-Bayoumy, N. Elmouhty, and H. Shafik
Human AFP was used as an antigen for the development of monoclonal antibodies by the hybridoma technique. Balb/c mice were
immunized with highly purified AFP. The preparation of I125-AFP was carried out by lactoperoxidase oxidation method, preparation of AFP standards was carried out from cord sera. The
antibody titer of the serum was tested by RIA-AFP system. The spleen of the immunized Balb/c was fused with Sp20 mouse myeloma cells. The cells from the positive hybridomas were cloned twice using limiting dilution method. Eleven stable
clones were thus established for secreting monoclonal antibodies to AFP. Cells in this growth phase were chosen for freezing.
Authors:K. Bapat, A. Korde, M. Zaidi, A. Mukherjee, M. Pillai, and M. Venkatesh
Prostate specific antigen (PSA) is a reliable biochemical marker used in screening for prostate carcinoma. Immunoradiometric assays (IRMA) are generally used for the estimation of total/free PSA in serum samples. Radiolabeled antibody, an important reagent of IRMA was prepared and characterized using an in-house anti-PSA monoclonal antibody (Mab), Mab-2S. Mab-2S was radiolabeled with 125I and characterized for immunoreactivity and radiochemical purity. The usability of the radioiodinated Mab as tracer in IRMA was ascertained using authentic reagents for IRMA of PSA.
Authors:Hafiz Abbas, Saeeda Asghar, and Maqbool Shahid
A method for labeling of anti-carcinoembryonic antigen (CEA) monoclonal antibody (MAb) BW 431/26 is described. For preparations of99mTc-anti-CEA complex, a solution (1 ml) of tetrasodium-1,1,3,3-propanetetetraphosphate dihydrate (2.7 mg), stannous chloride dihydrate (0.12 mg) and sodium chloride (0.2 mg) in 5 ml of 0.9% saline was added to a vial containing monoclonal antibody (2 mg) from mouse (MAb BW 431/26), D-glucitol (2 mg) and sodium sulfate (2 mg) to obtain a clear solution. A quantitative labeling yield of the MAb BW 431/26 was achieved by addition of 5 ml (40.5 mCi, 1.5 Gbq) technetium-99m-pertechnetate (99mTcO4) generator eluate at room temperature within 10 minutes. The radiochemical purity determined by ITLC (Gelman, SG) plates was >95%.
Authors:P. C. Vrinda, S. N. Paradkar, U. H. Nagvekar, G. Samuel, and N. Sivaprasad
A simple, one step, inclusive immunoradiometric assay for human chorionic gonadotropin (hCG) employing monoclonal antibodies
is described. Commercially available monoclonal antibodies from various commercial sources were screened. Identified “detection”
antibody was radiolabeled with 125I and the selected “capture” antibody was chemically coupled to magnetizable cellulose to form a solid phase. In the procedure
developed, standard/sample, radiolabeled antibody and capture antibody were incubated together for 3 hours at room temperature
with shaking. After incubation, the bound complex was quantitated for the associated radioactivity. The analytical sensitivity
observed was 1.0 mIU/ml with a wide concentration range up to 1000 mIU/ml of hCG. “High dose hook” of the developed assay
was observed beyond 2000 mIU/ml. Results showed that the developed assay had a good precision: intra-assay CV less than 8%,
inter-assay CV less than 10% and good analytical recovery of 97-109%. The clinical samples analyzed by the developed procedure
showed a good correlation with that of the commercial kit (r = 0.92; y = 0.99x+0.51).