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Acta Chromatographica
Authors:
Yongxi Jin
,
Yuyan Chen
,
Jiawen Liu
,
Xi Bao
,
Yinghao Zhi
,
Congcong Wen
, and
Wenzong Zhu

–tandem mass spectrometry (UPLC–MS/MS) reported for the determination of ebeiedinone. To the best of our knowledge, the pharmacokinetics of ebeiedinone had not been reported. In this paper, UPLC–MS/MS method was established to determine ebeiedinone in mouse

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Acta Chromatographica
Authors:
Shanjiang Chen
,
Miaoling Huang
,
Zheng Yu
,
Jiamin He
,
Binge Huang
,
Xianqin Wang
,
Jianshe Ma
, and
Congcong Wen

analyzing the in vivo metabolism of complicated traditional Chinese medicine (TCM) components and complex compound systems [ 11 , 12 ]. In this study, we established an analytical method to detect the concentration of 8- O -acetylharpagide in mouse blood

Open access

reports about the pharmacokinetics of eugenitin in biological fluids. Therefore, it was necessary to develop a UPLC-MS/MS method for the pharmacokinetics. In this study, UPLC-MS/MS was used to detect eugentin in mouse blood and its pharmacokinetics was

Open access
Acta Veterinaria Hungarica
Authors:
B. Baranyai
,
Sz. Bodó
,
A. Dinnyés
, and
Elen Gócza

Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35% EG + 5% PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vi tro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.

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153 157 Johnson JW, Ascher P: Glycine potentiates the NMDA response in cultured mouse brain neurons. Nature 325, 529–531 (1987) Ascher

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recognized as an effective method for modern pharmacokinetic study [ 11 ]. In this study, based on UPLC-MS/MS, a simple and rapid analytical method was developed for the determination of spiraeoside in mouse blood and then applied to study the

Open access
Acta Physiologica Hungarica
Authors:
Z Kojić
,
Z Kojić
,
Z Kojić
,
Lj Šćepanović
,
Lj Šćepanović
,
Lj Šćepanović
,
N Popović
,
N Popović
, and
N Popović

In our previous work we have shown that in mouse heart basal level of endothelial produced nitrite, as a marker of nitric oxide (NO) formation, was 9.7 nmol l-1. Bradykinin (10 mmol l-1) induced a 5-fold rise in nitrite release, the coronary venous effluent concentration being 58 nmol l-1, but there was no effect on myocardial oxygen consumption (MVO2). The aim of this study was to assess the levels of authentic nitric oxide solution, exogenously applied, on myocardial oxygen consumption. Isolated mouse hearts (n=36) were paced (500 imp./min) and perfused at constant flow (16.0±0.3 ml g-1 min-1). When coronary vasculature resistance was carefully controlled by adenosine (1 mmol l-1), authentic nitric oxide solution, in a concentration less than 5 mmol l-1 did not alter myocardial oxygen consumption. Only concentrations of nitric oxide higher than 5 mmol l-1 induced reduction in myocardial oxygen consumption. Thus in the saline perfused mouse heart, with carefully controlled vasodilatation, modulating myocardial nitric oxide levels using an arterial application of authentic nitric oxide, concentrations higher than 5 mmol l-1 of nitric oxide were required to induce a decrease in myocardial oxygen consumption.

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- O -beta-gentiobioside, and guaijaverin in mouse blood and then used this method to study the pharmacokinetics after intravenous administration. Experimental Chemicals and animals

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The present study was designed to investigate fertilisation of open pulled straw (OPS) vitrified mouse oocytes drilled with piezo-micromanipulation method and their subsequent in vitro and in vivo developmental capacity. Ovulated mouse oocytes were vitrified using the OPS method. After warming, the zona pellucida of a group of vitrified-warmed oocytes was drilled by piezo-micro-manipulation. Groups of (a) vitrified, (b) vitrified/drilled and (c) fresh control oocytes were fertilised in vitro . The fertilisation rate of vitrified-warmed oocytes was significantly lower than that of fresh oocytes (45.0 ± 12.6% vs. 85.2 ± 6.8%, P < 0.05), and was significantly improved by zona-drilling (85.4 ± 7.3%). However, blastocyst formation rates of the vitrified and vitrified/drilled groups were significantly lower than those of the fresh controls (65.7 ± 7.0% and 66.4 ± 2.5% vs. 86.6 ± 4.3%, respectively, P < 0.05). The cell number of blastocysts from the vitrified/drilled or the vitrified group was not different from that of the controls. Embryo transfer resulted in pregnancy in all three groups, but the rate of development to term was lower in the vitrified/drilled or vitrified groups than in the controls (16.6 ± 0.7% or 36.0 ± 2.4% vs. 51.3 ± 2.9%, respectively). Our results demonstrated that zona-drilling with piezo-micromanipulation could improve fertilisation in OPS vitrified mouse oocytes but did not increase the overall number of vitrified oocytes developing to term.

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Acta Veterinaria Hungarica
Authors:
Chang-Liang Yan
,
Qi-En Yang
,
Guang-Bin Zhou
,
Yun-Peng Hou
,
Xue-Ming Zhao
,
Zhi-Qiang Fan
,
Man-Qing Liu
,
Lin Liu
, and
Shi-En Zhu

The present study was designed to investigate the cryotolerance of in vitro fertilised (IVF) mouse embryos at various preimplantation developmental stages. IVF mouse embryos were vitrified by the open-pulled straw (OPS) method. After warming, embryos were morphologically evaluated and assessed by their development to blastocysts, hatched blastocysts or term. The results showed that a high proportion (93.3–100.0%) of vitrified embryos at all developmental stages were morphologically normal after recovery. The developmental rate of vitrified 1-cell embryos to blastocyst (40.0%) or hatched blastocyst (32.7%) or term (9.3%) was significantly lower than that from other stages (P < 0.05). Vitrified embryos from 2-cell to early blastocyst stage showed similar blastocyst (71.8–89.5%) and hatched blastocyst rates (61.1–69.6%) and could develop to term without a significant loss of survival compared with those of fresh embryos (P > 0.05). Vitrified 2-cell embryos showed the highest survival rate in vivo (50.6%, 88/174), compared with that from other stages (9.3–30.5%, P < 0.05). The data demonstrate that the OPS method is suitable for the cryopreservation of IVF mouse embryos from 2-cell stage to early blastocyst stage without a significant loss of survival. Embryos at the 2-cell stage had the best tolerance for cryopreservation in the present study.

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