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A szerző új, a vörösvérsejt hemoglobintartalmára alapozott algoritmust mutat be. A rendszer nem az anaemia kórtanán, hanem a vörösvérsejtképzés eltérésein alapul. Egy vörösvértestben átlag 28–35 pikogramm hemoglobin van. Ha a hemoglobinképzés bármely eleme sérül, a hemoglobintartalom kevesebb mint 28 pikogrammra csökken és hipokróm anaemia jön létre. Ha a vörösvérsejt nukleinsav-anyagcseréje sérül, akkor egy vörösvértest hemoglobintartalma 36 pikogramm vagy több, és hiperkróm anaemia alakul ki. A 28 és 35 pikogramm közötti hemoglobintartalmú normokróm anaemiát az erythropoesis proliferációs eltérései hozhatják létre. A fenti 3 kategória alapján egységes rendszer alkotható és az alapvető laboratóriumi vizsgálatok, illetve a betegek kivizsgálási útjai is modellálhatók. Orv. Hetil., 2014, 155(10), 376–382.
Canine babesiosis is a frequent and clinically significant tick-borne disease. Sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in Serbia. After clinical examination, blood samples were taken for microscopic examination, complete blood count (CBC), Canine SNAP 4Dx Test, DNA analyses and sequencing. The main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, and vomiting. The main clinicopathological findings in Babesia infections were a slight to severe thrombocytopenia and a mild to very severe normocytic normochromic anaemia. Microscopic evaluation revealed 58 positive samples with the presence of large and small intraerythrocytic piroplasms in 57 and 1 sample(s), respectively. No co-infections were found using SNAP test. Two Babesia species, B. canis (58/60) and B. gibsoni (2/60), were differentiated by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). Species identification was further confirmed by sequencing PCR products of B. gibsoni samples and six randomly selected B. canis samples. All dogs were treated with imidocarb dipropionate (6.6 mg/kg of body weight), given intramuscularly twice at an interval of 14 days. This report presents the first molecular evidence of the occurrence of B. gibsoni and B. canis, confirmed by DNA sequencing, in sick dogs from Serbia.
