Authors:Petra Zenke, Á. Maróti-Agóts, Zs. Pádár, and L. Zöldág
Demand for correct and cost-effective genetic-based identification and parentage control has increasing importance in domestic animals, including dogs. In our study the applicability of a canine hyperpolymorphic microsatellite marker — which localized in the WILMS-TF (tumor factor) gene — was examined in mixed breed and purebred canine populations. The redesigned and shortened amplicons were genotyped using an allelic ladder which was constructed from sequence verified fragments. The nomenclature for allele calling based on repetition structures is suitable for international comparisons. Our study justified the potential use and efficiency of the marker D18S12 in parentage control.
Authors:Z. Pádár, B. Egyed, K. Kontadakis, L. Zöldág, and S. Fekete
A case of disputed paternity in dogs is reported. DNA examinations were carried out from hair samples of the individuals several months after the death of the putative sire. Ten short tandem repeat (STR) loci were analysed by fluorescence- labelled multiplex PCR using ABI PRISM 310 Genetic Analyser. Based on the results the candidate sire was included in the pedigree records as the biological sire. In spite o f the genetic homogeneity of pedigree dogs due to inbreeding, canine microsatellites can provide an adequate basis for assigning paternity in pure breeds.
Authors:Krisztián Frank, Endre Barta, Nóra Á. Bana, János Nagy, Péter Horn, László Orosz, and Viktor Stéger
. , Lehoczky , I. , Gyurmán , A. , Nagy , J. , Sugár , L. , Anton , I. , Horn , P. , Magyary , I. ( 2009 ) Development of eight-plex microsatellite PCR for parentagecontrol in deer . Archiv Tierzucht 52 , 143 – 149 .