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European Journal of Microbiology and Immunology
Authors:
B. Neelakantam
,
N. V. Sridevi
,
A. M. Shukra
,
P. Sugumar
,
S. Samuel
, and
Dr. Lingala Rajendra PhD

K. Rangnoi P. Kristensen M. Yamabhai 2009 A compact phage display human scFv library for

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In this study, the phage adsorption inhibition type resistance system was investigated in 6 bacteriocin producing strains, Lactococcus lactis subsp. lactis BLL10, BLL27, BLL31, BLL84 and BLL90 and L. lactis subsp. cremoris BLC67. All six bacteriocin producing strains were determined to comprise phage adsorption inhibition type of resistance against three phages (Øpll98-28, Øpld67-41 and Øpld67-43). Genetic determinants of these two systems were also analysed in these strains. The bacteriocin production abilities and phage adsorption inhibition type resistance of these strains were found to be determined by 13.4 kb and 25.3 kb plasmids in BLL10; 9.5 kb and 30.1 kb plasmids in BLL27; 10.4 kb and 29.0 kb plasmids in BLL31; 23.4 kb and 19.0 kb plasmids in BLL84; 7.5 kb and 15.3 kb plasmids in BLL90, respectively. In BLC67, both characteristics were found to be determined by 31.3 kb plasmid. Conjugal mating experiments showed that 30.1 kb plasmid in BLL27, 29.0 kb plasmid in BLL31, 23.4 kb plasmid in BLL84 and 31.3 kb plasmid in BLC67 were conjugally transferable with the frequencies of 3.6×10 −3 , 8.2×10 −4 , 1.4×10 −6 and 6.6×10 −3 , respectively.

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specifically tackle bacteria, represent a possible part of the solution to this threat to humankind. Initially, as first comprehensively described by the French microbiologist Félix d’Hérelle in 1917 [ 6 , 7 ], phages were widely used to combat bacterial

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knee prostheses will be affected by an infection with various symptomatic manifestations [ 1 – 3 ] ( Fig. 1 ). Fig. 1. Core symptoms of a prosthetic joint infection and the principle of phage therapy The quarter-split circle illustrating core symptoms

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) 10. Kenzaka T , Tani K , Nasu M : High-frequency phage-mediated gene transfer in freshwater environments determined at single-cell level . ISME J 4 , 648 – 659

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cells using its long tail fibers, which recognize and bind to lipopolysaccharides and OmpC on the cell surface [ 24 ]. This specific interaction allows the T4 phage to inject its genetic material into the bacterium, initiating replication. The

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validated the epitope by four in silico programs, Jped4, Clustal Omega, TMpred and SOSUI ( Cuff et al., 1998 ; Hirokawa et al., 1998 ; Sievers and Higgins, 2014 ). In addition, we performed phage display and screened it for antibodies to chicken IFITM3

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98 Lactococcus lactis strains were isolated from traditional fermented milk products in Turkey tested against 60 lactococcal lytic phages to determine their resistance levels. While 82 L. lactis strains were sensitive against lactic phages at different levels, 16 L. lactis strains showed resistance to all phages tested. Types of phage resistance among 16 L. lactis strains were identified as phage adsorption inhibition in eight strains, restriction/modification in six strains and abortive infection (heat sensitive phage resistance) in two strains, using three broad-spectrum phages Fpll 98-32, Fpld 67-42 and Fpld 67-44.

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Acta Microbiologica et Immunologica Hungarica
Authors:
József Klem
,
Dóra Dömötör
,
György Schneider
,
Tamás Kovács
,
András Tóth
, and
Gábor Rákhely

The emergence of the multi-drug-resistant Staphylococcus aureus strains has prompted interest in alternatives to conventional drugs. Among the possible options one of the most promising is the therapeutic use of bacteriophages. Over the recent decades, increasing amount of literature has validated the use of bacteriophages for therapy and prophylaxis against drug-resistant staphylococci. This work attempts to review the current knowledge on bacteriophages and their usages for treatment of staphylococcal diseases.

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for horizontal gene transfer [ 5, 10 ]. During the past decade, sequencing of Staphylococci genomes and improvements in the comparative genomic analyses has significantly increased the number of staphylococcal phages identified. To date, more

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