.11 4.41 105.27 Pharmacokinetic application The method for the determination of rat plasma, pharmacokineticstudy and bioavailability of sarecycline was successfully applied in rats, with the drug-time curve shown in Fig. 3 . The pharmacokinetic
Authors:Xiaorong Wu, Yankai Wang, Binbin Liang, Honghai Wu, Liying Wu, Jing Song, and Jianfang Liu
pharmacokineticstudies of these preparations have been conducted in many countries, however, the pharmacokineticstudies of fenofibrate tablets (Lipanthyl® supra, 160 mg) in Chinese subjects have not been reported. Ultra-high performance liquid chromatography
Authors:G. Raszewski, M. Juszczak, M. K. Lemieszek, J. Matysiak, A. Niewiadomy, and W. Rzeski
The development, optimization, and validation of a new high-performance liquid chromatography ultraviolet (HPLC-UV) method is presented for determining 2-(3-chlorophenyloamino)-5-(2,4-dihydroxy-phenyl)-1,3,4-thiadiazole (ClABT) in biological samples of rat plasma and brain tissue. ClABT was extracted directly from a plasma supernatant fraction following protein precipitation with acetonitrile and high speed centrifugation. Reverse phase HPLC separation was performed using an ODS-2 Hypersil column with the mobile phase consisting of 0.05 M triethylammonium phosphate buffer solution in acetonitrile and methanol (120:280:600, v/v/v), at room temperature, 1.2 mL min−1 flow rate, and UV-diode-array detection (DAD), at 335 nm. A linear response was obtained between 12.5 and 2000 ng mL−1 at analytically acceptable levels of precision (intra/inter-day) and accuracy. Mean recoveries ranged from 92.7% to 107.9%. It was concluded that the method was specific and precise and thus suitable for quantitative analysis in clinical pharmacokinetic studies of ClABT.
Authors:J.R. Li, D.X. Li, L. Li, W.L. Deng, L.S. Ding, H.X. Xu, and Y. Zhou
A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL−1 ng/mL for tanshinone IIA and 100 pg/mL−1 for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.
successfully applied to a comparative pharmacokineticstudy of 10 alkaloids in rat plasma after oral administration of Rhizoma Menispermi extract [ 9 ]. Liu et al. developed a liquid chromatography–tandem mass spectrometry method for quantitative
Authors:Libin Wang, Le Mi, Tian Feng, Xueying Liu, and Shengyong Zhang
generate and store triglycerides, to achieve the purpose of disease prevention and cure [ 26 , 27 ].
Due to the therapeutic effects of PH, the pharmacokineticstudies of PH are essential. Some previous studies have shown that the poor
. Long-term stability was assessed after storage of the standard spiked plasma samples at −20 °C for 20 days. The stability of the IS (50 ng mL −1 ) was evaluated similarly [ 36 ].
Authors:Aixia Han, Guanyang Lin, Jinzhang Cai, Qing Wu, Peiwu Geng, Jianshe Ma, Xianqin Wang, and Chongliang Lin
times, from −20 °C to room temperature).
Diet was prohibited for 12 h before the experiment while water was freely available. Twenty-four SD rats (200–220 g) were numbered as 1–24 and divided into
Authors:Ke Ren, Tiantian Feng, Hai Shi, Jianshe Ma, and Yongxi Jin
preparation One hundred microliter acetonitrile (containing IS 100 ng/mL) was added into 20 μL blood, mixed for 1.0 min, and centrifuged at 13,000 rpm for 10 min. The supernate (2 μL) was injected into UPLC-MS/MS for analysis. Pharmacokineticstudy Six
Authors:Jong-Woo Jeong, Yun-Hwan Seol, Hun-Chan Hyun, Hye-Rim Kim, Jong-Hwa Lee, Young-Dae Gong, Nam Sook Kang, and Tae-Sung Koo
chromatography–tandem mass spectrometry (LC–MS/MS). This method was successfully used in the pharmacokineticstudies of supinoxin in rats and may be useful for the development of clinical data.