Authors:M. Then, K. Szentmihályi, A. Gere, Sz. Jasztrab, and É. Szőke
In vitro investigations for the antioxidant and free radical scavenging activity of Myrtilli folium-, Phaseoli fructus sine seminibus- and a drug mixture (Equiseti herba, Myrtilli folium, Phaseoli fructus sine seminibus, Urticae folium) extracts showed antioxidant (LPO inhibitory and chain-breaking antioxidant) activity and free radical (superoxide anion and hydroxyl radical) scavenging effect. The extracts inhibited lipid peroxidation induced enzymatically by adding NADPH and non-enzymatically by adding Fe2+ in brain microsomes and in brain homogenates, respectively. The extracts reduced the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), which showed chain-breaking antioxidant activity. The extracts scavenged superoxide radicals (O2-·) by inhibiting the reduction of nitro blue tetrazolium evoked by phenazine methosulphate. In addition, the extracts inhibited Fenton-reagent (Fe2+ and H2O2) induced deoxyribose degradation, therefore, it was concluded that the extracts have hydroxyl radical (OH·) scavenging property.
Serum LD activity is increased in several diseases. The clinical significance of elevated LD may be evaluated by isoenzyme analysis. Here, we report on a long-term elevation of serum LD activity with a rare isoenzyme pattern caused by LD-IgG complexes.
A 75-year old female with atherosclerosis and implantations of two stents showed increased LD activity and an unusual isoenzyme pattern. LD activity, inhibition and immunoglobulins were measured with Roche tests; immunofixation was performed with a Helena test. LD isoenzymes were separated in agarose gel and quantified by the color formed by NADH, reduced phenazine methosulfate and nitro blue tetrazolium.
LD isoenzyme analyses revealed one band near the slowly migrating LD-4 position. The band contained LD complexed with immunoglobulins (IgG with κ light chains), The long-term increased serum LD enzyme activity in this patient may have been due to abnormally slow clearance of these large macro-LD complexes from circulation.
An increased LD activity and a rare LD-IgG pattern were detected for five years in a patient who had not rare diseases but atherosclerosis and two stents. Evaluation of LD activity and its isoenzyme test results may be complicated and misleading for patients with the same LD-IgG complex in their serum.
Authors:Marijana Kosanić, Branislav Ranković, and Tatjana Stanojković
Nishimiki , M. , Rao , N. A. , Yagi , K. ( 1972 ) The occurrence of super-oxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen . Biochem. Biophys. Res. Commun. 46 , 849 – 853 .
Authors:Julia S. Yumnam, Mayank Rai, and Wricha Tyagi
. , Peever , T. L. , Mavrodi , O. V. , Parejko , J. A. , Raaijmakers , J. M. , Lemanceau , P. , Mazurier , S. , Heide , L. , Blankenfeldt , W. , Weller , D. M. , Thomashow , L. S. ( 2010 ) Diversity and evolution of the phenazine
Authors:B. Baráti-Deák, Á. Belák, and Cs. Mohácsi-Farkas
fluorescent pseudomonads – have antagonistic ability against different foodborne pathogens ( Alegre et al., 2013; Olanya et al., 2014; Belák & Maráz, 2015; Oliveira et al., 2015 ). Secondary metabolites of fluorescent Pseudomonas species, such as phenazines
Authors:A. Durak, I. Kowalska, and U. Gawlik-Dziki
initiated by adding 100 μL of phenazine methosulfate (PMS) solution to the reaction mixture. Samples were incubated for 5 min at 25 °C, and the absorbance at 560 nm was measured against blank samples. The decreased absorbance of the reaction mixture
Authors:Mohan N. Patel, Bhupesh S. Bhatt, and Promise A. Dosi
pairs. As increasing the amounts of complexes, viscosity of DNA increases steadily, which is similar to that of the classical intercalative complex [Ru(phen) 2 (DPPZ)] 2+ where DPPZ is dipyrido [3,2-a:2,3-c]phenazine. The results suggest that the all