Porcine epidemic diarrhoea virus (PEDV) is one of the important pathogens that may cause severe diarrhoea in piglets. In this study, the nucleocapsid (N) gene of a Chinese PEDV isolate designated HLJBY was cloned. The phylogeny of PEDV strains was investigated by constructing a phylogenetic tree based on the N protein sequences. The results indicate that there are two major groups of Chinese PEDVs, a Japanese PEDV group and a Korean PEDV group. High-level expression of the N protein was achieved in Escherichia coli. The immunoreactivity between PEDV particles or the bacterially expressed N protein and rabbit anti-PEDV serum was confirmed by immunofluorescence assays and Western blot. Both PEDV N protein and the polyclonal antibody generated in this study are valuable diagnostic reagents for PEDV surveillance.
Dolja, V. V., Boyko, V. P., Agranovsky, A. A., Koomin, E. V. (1991) Phylogeny of the capsid proteins of rod-shaped and filamentous RNA plant viruses: two families with distinct patterns of sequence and probably structure conservation. Virology 184 , 79
Authors:S. Kondratyuk, I. Kärnefelt, A. Thell, J. Elix, J. Kim, M.-H. Jeong, N.-N. Yu, A. Kondratiuk, and J.-S. Hur
The phylogeny of the subfamily Xanthorioideae (Teloschistaceae) is re-analysed based on ITS, LSU and mtSSU sequences, including a new set of specimens representing 31 genera, of which five are proposed as new: Golubkovia, Igneoplaca, Langeottia, Scythioria and Verrucoplaca. Two new species, Ovealmbornia volkmarwirthii from South Africa and Gondwania sejongensis from Antarctica are described, illustrated and compared with closely related taxa. Eleven new combinations are proposed in the genera Calogaya, Cerothallia, Flavoplaca, Gondwania, Igneoplaca, Scythioria and Verrucoplaca, but the status of four earlier established genera, Pachypeltis, Parvoplaca, Solitaria and Xanthopeltis, remains uncertain and needs further studies.
Authors:S. Y. Kondratyuk, L. Lőkös, S.-H. Jang, J.-S. Hur, and E. Farkas
From the combined phylogenetic analysis of multi-locus sequence data of the Lecanoraceae including two nuclear protein-coding markers (RPB2 and RPB1), the internal transcribed spacer and a fragment of the mitochondrial small subunit, found that the originally monotypic eastern Asian genus Verseghya is positioned within the Verseghya-Lecidella-Pyrrhospora clade of the Lecanoraceae and includes one more taxon Verseghya thysanophora widely distributed in Northern Hemisphere.
The genus Lecidella forming the Lecidella-Glaucomaria subclade within the same Verseghya-Lecidella-Pyrrhospora clade of the Lecanoraceae found to have tendency to be polyphyletic after including the recently described eastern Asian taxon Lecidella mandshurica into phylogenetic analysis of the Lecanoraceae. It is shown that Lecidella mandshurica was previously recorded from China sub Lecidella aff. elaeochroma.
The originally monotypic eastern Asian genus Sedelnikovaea forming a monophyletic branch within the Sedelnikovaea-Lecanoropsis subclade and being in out-position to the Rhizoplaca-Protoparmeliopsis s. str. clade of the Lecanoraceae found to include three more taxa, i.e. Sedelnikovaea marginalis, S. pseudogyrophorica, and S. subdiscrepans.
The Eurasian Protoparmeliopsis bolcana, and the eastern Asian P. kopachevskae, are illustrated for the first time as being positioned within the Protopameliopsis branch of the Lecanoraceae, while the South Korean ‘Protoparmeliopsis’ chejuensis found to be positioned in separate monophyletic branch from all other branches of the Rhizoplaca-Protoparmeliopsis s. l. clade of the Lecanoraceae.
The genus Polyozosia A. Massal. as earlier name for the former Myriolecis branch of the Lecanoraceae is accepted as far the type species of the latter genus, i.e. P. poliophaea, found to be positioned within this branch. The Polyozosia robust monophyletic branch is positioned in the outermost position in the Rhizoplaca-Protoparmeliopsis s. str. clade of the Lecanoraceae.
Position and species content of the accepted genera Glaucomaria, Lecanoropsis, Omphalodina, Polyozosia, and Straminella are discussed in separate nrITS and mtSSU, and combined phylogeny based on concatenated sequences of nrITS, mtSSU, RPB2 and RPB1 genes.
Fourty new combinations are proposed: Glaucomaria bicincta, G. carpinea, G. leptyrodes, G. lojkaeana, G. subcarpinea, G. sulphurea, G. swartzii, G. swartzii subsp. caulescens, G. swartzii subsp. nylanderi, Lecanoropsis anopta, L. macleanii, Omphalodina chrysoleuca, O. huashanensis, O. opiniconensis, O. phaedrophthalma, O. pseudistera, Palicella anakeestiicola, Polyozosia albescens, P. andrewii, P. contractula, P. crenulata, P. dispersa, P. hagenii, P. perpruinosa, P. populicola, P. pruinosa, P. reuteri, P. sambuci, P. semipallida, P. straminea, P. thuleana, Sedelnikovaea marginalis, S. pseudogyrophorica, S. subdiscrepans, Straminella bullata, S. burgaziae, S. conizaeoides, S. densa, S. maheui, S. varia, and Verseghya thysanophora. Validation of one name as Polyozosia perpruinosa Fröberg ex S. Y. Kondr. L. Lőkös et Farkas is also proposed.
Authors:Márton Z. Vidovszky, Claudia Kohl, Sándor Boldogh, Tamás Görföl, Gudrun Wibbelt, Andreas Kurth, and Balázs Harrach
Juste , J. , Benda , P. , Garcia- Mudarra , J. J. and Ibá nez , C. ( 2013 ): Phylogeny and systematics of Old World serotine bats (genus Eptesicus, Vespertilionidae, Chiroptera): an integrative approach . Zool. Scr . 42 , 441 – 3
Authors:S. Y. Kondratyuk, L. Lőkös, M.-H. Jeong, S.-O. Oh, A. S. Kondratiuk, and J.-S. Hur
Introduction Molecular phylogeny of the representatives of the Candelariaceae are hitherto provided for separate genera, like Candelaria (Liu and Hur 2018, Westberg et al. 2007), and Candelariella (Kondratyuk et al. 2014a, Liu et al. 2019
Authors:S. Y. Kondratyuk, L. Lőkös, E. Farkas, I. Kärnefelt, A. Thell, Y. Yamamoto, and J.-S. Hur
. , Stenroos , S. , Thell , A. , Kärnefelt , I. , Elix , J. A. , Hur , J. S. and Kondratyuk , S. Y. ( 2012 ): Molecular phylogeny of xanthorioid lichens (Teloschistaceae, Ascomycota), with notes on their morphology . – Bibl. Lichenol. 108 : 45
Authors:S. Y. Kondratyuk, L. Lőkös, I. Kärnefelt, A. Thell, M.-H. Jeong, S.-O. Oh, A. S. Kondratiuk, E. Farkas, and J.-S. Hur
The nrITS is hitherto the most frequently used molecular marker used for phylogeny of species and genera in the Physciaceae, followed by the 12S mtSSU. Other markers have been used to a much lesser extent
Authors:S. Y. Kondratyuk, L. Lőkös, A. S. Kondratiuk, I. Kärnefelt, A. Thell, E. Farkas, and J.-S. Hur
INTRODUCTION Even though numerous molecular data on the members of the Trapeliaceae and Xylariaceae were accumulated during last two decades (Ertz et al . 2014, Orange 2018, Resl et al . 2015, 2018, Schneider et al . 2016) phylogeny of the total