Authors:R. Singh, P. Hucl, M. Båga, and R. Chibbar
Pre-harvest sprouting (PHS) in bread wheat (Triticum aestivum L.) is one of the major abiotic constraints influencing production of high quality grain. Selection for pre-harvest sprouting (PHS) resistance in bread wheat (Triticum aestivum L.) in early generations is difficult because it is expressed as a quantitatively inherited trait and subject to environmental effects. The objectives of this study were to validate a major quantitative trait locus (QTL) for PHS resistance on chromosome 4A in bread wheat and to isolate near-isogenic lines for this QTL using marker-assisted selection. A total of 60 Canadian wheat cultivars and experimental lines were screened with three SSR markers in a QTL region for PHS resistance. The SSR markers DuPw004, barc170 and wmc650 explained 67%, 75% and 60% of total variation in germination (%), respectively, among different wheat genotypes. Marker assisted back crossing with DuPw004 reduced the population size in BC1F1 and BC2F1 generation by 41% and 59%, respectively. A survey of pedigrees of different genotypes revealed that the parental line RL4137 is a major source of increased PHS resistance in a number of western Canadian wheat cultivars. Microsatellite markers (DuPw004, barc170 and wmc650) will be useful for plant breeders to pyramid QTL from different PHS resistance sources.
Authors:Y. Yang, X. Chen, Z. He, M. Röder, and L. Xia
Genotypes with various
alleles perform different levels of pre-harvest sprouting (PHS) tolerance. In this study, 217 white-grained wheat cultivars, including 75 landraces, 39 historical cultivars, and 103 modern cultivars from five major regions of China, were examined to characterize the diversity of the Viviparous-1B (
) locus associated with PHS tolerance. Four
alleles were identified, three (
) of which were previously reported in Chinese wheat cultivars. A new allele,
, was identified in the PHS tolerant landrace Hongheshangtou. Sequence analysis showed that
had an insertion of a 4-bp fragment, two SNPs, and a deletion of an 83-bp fragment compared with the nucleotide sequence of
(AJ400713), all located in the third intron.
shared 97.80% similarity with the nucleotide sequence of AJ400713. The frequencies of
were 36.0%, 5.3%, and 57.3% in landraces; 23.1%, 7.7%, and 69.2% in historical cultivars; and 52.4%, 0%, and 47.6% in current cultivars, respectively.
Authors:Slobodan Tomasovic, Rade Mlinar, Ivica Ikic, Katarina Jukic, and Branko Palaversic
sprouting, dormancy and germination inhibitors in bracts of 50 diverse wheat genotypes — 8. International Symposium on Pre-HarvestSprouting in Cereals — Weipert D. (ur.) Detmold: Association of Cereal Research, Germany, 1999: 39–49 pp
Authors:D. Obreht, B. Kobiljski, S. Denčić, M. Djan, and Lj. Vapa
Gross C., Gay G., Perretant M.-R., Gervais L., Bernard M., Dedryver F., Charmet G. (2002) Study of the relationship between pre-harvestsprouting and grain colour by quantitative trait loci analysis in white x red grain bread-wheat cross. Theor. Appl