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Pre-harvest sprouting (PHS) in bread wheat (Triticum aestivum L.) is one of the major abiotic constraints influencing production of high quality grain. Selection for pre-harvest sprouting (PHS) resistance in bread wheat (Triticum aestivum L.) in early generations is difficult because it is expressed as a quantitatively inherited trait and subject to environmental effects. The objectives of this study were to validate a major quantitative trait locus (QTL) for PHS resistance on chromosome 4A in bread wheat and to isolate near-isogenic lines for this QTL using marker-assisted selection. A total of 60 Canadian wheat cultivars and experimental lines were screened with three SSR markers in a QTL region for PHS resistance. The SSR markers DuPw004, barc170 and wmc650 explained 67%, 75% and 60% of total variation in germination (%), respectively, among different wheat genotypes. Marker assisted back crossing with DuPw004 reduced the population size in BC1F1 and BC2F1 generation by 41% and 59%, respectively. A survey of pedigrees of different genotypes revealed that the parental line RL4137 is a major source of increased PHS resistance in a number of western Canadian wheat cultivars. Microsatellite markers (DuPw004, barc170 and wmc650) will be useful for plant breeders to pyramid QTL from different PHS resistance sources.

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associated with resistance to pre-harvest sprouting in wheat. Crop Sci. 33 :453–459. Tanksley S.D. RFLP analysis of genomic regions associated with resistance to pre-harvest sprouting

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Abbreviations PHS pre-harvest sprouting PM physiological maturity

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Genotypes with various Vp-1B alleles perform different levels of pre-harvest sprouting (PHS) tolerance. In this study, 217 white-grained wheat cultivars, including 75 landraces, 39 historical cultivars, and 103 modern cultivars from five major regions of China, were examined to characterize the diversity of the Viviparous-1B ( Vp-1B ) locus associated with PHS tolerance. Four Vp-1B alleles were identified, three ( Vp-1Ba , Vp-1Bb and Vp-1Bc ) of which were previously reported in Chinese wheat cultivars. A new allele, Vp-1Be , was identified in the PHS tolerant landrace Hongheshangtou. Sequence analysis showed that Vp-1Be had an insertion of a 4-bp fragment, two SNPs, and a deletion of an 83-bp fragment compared with the nucleotide sequence of Vp-1Ba (AJ400713), all located in the third intron. Vp-1Be shared 97.80% similarity with the nucleotide sequence of AJ400713. The frequencies of Vp-1Ba , Vp-1Bb , and Vp-1Bc were 36.0%, 5.3%, and 57.3% in landraces; 23.1%, 7.7%, and 69.2% in historical cultivars; and 52.4%, 0%, and 47.6% in current cultivars, respectively.

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Auld, A.S., Paulsen, G.M. 2003. Effect of drought and high temperature during maturation on pre-harvest sprouting hard white winter wheat. Cereal Res. Comm. 31 :169

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. 2003 . An approach to identification of rye chromosomes affecting the pre-harvest sprouting in triticale . J. Appl. Genet. 44 : 491 – 496 . Silkova , O.G. , Kabanenko, Yu , N

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711 729 Anderson, J.A., Sorells, M.E., Tanksley, S.D. 1993. RFLP analysis of genomic regions associated with resistance to pre-harvest sprouting in wheat. Crop Sci. 33 :453

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Cereal Research Communications
Authors: Slobodan Tomasovic, Rade Mlinar, Ivica Ikic, Katarina Jukic, and Branko Palaversic

sprouting, dormancy and germination inhibitors in bracts of 50 diverse wheat genotypes — 8. International Symposium on Pre-Harvest Sprouting in Cereals — Weipert D. (ur.) Detmold: Association of Cereal Research, Germany, 1999: 39–49 pp

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Lohwasser, U., Röder, M.S., Börner, A. 2005. QTL mapping of the domestication traits pre-harvest sprouting and dormancy in wheat ( Triticum aestivum L.). Euphytica 143 :247–249. Börner A

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Gross C., Gay G., Perretant M.-R., Gervais L., Bernard M., Dedryver F., Charmet G. (2002) Study of the relationship between pre-harvest sprouting and grain colour by quantitative trait loci analysis in white x red grain bread-wheat cross. Theor. Appl

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