Authors:M. Rajabi Hashjin, M.H. Fotokian, M. Agahee Sarbrzeh, M. Mohammadi, and D. Talei
Knowledge of morpho-protein patterns of genetic diversity improves the efficiency of germplasm conservation and development. The objective of present study was to evaluate 116 genotypes of Triticum turgidum from seven countries in terms of morphological traits and seed protein banding patterns. The results showed highly significant differences among the genotypes for the traits. The correlation between grain yield and weight per spike was significant and positive, while the correlation between days to heading, length of peduncle and plant height was significant and negative. The factor analysis classified the traits in to four main groups which accounted for 74.4% of the total variability. Sixteen allelic compositions were identified in the genotypes for high molecular weight glutenin subunits. The three alleles were present at the Glu-A1 locus and 8 alleles at Glu-B1. The null allele was observed more frequently than the 1 and 2 alleles. Two alleles, namely 17 + 18 and 20, represented more frequent alleles at Glu-B1 locus. The genetic variability in Glu-A1and Glu-B1 loci were 0.42 and 0.81, respectively. The cluster analysis based on morphological traits and HMW-GS clustered the genotypes in to six and seven groups, respectively. The results indicated the presence of high genetic variability among the genotypes. Our findings suggest that the plants belong to different clusters can be used for hybridization to generate useful recombinants in the segregating generations, the genetics and breeding programs for improvement of durum wheat.
Authors:S. Peighambardoust, M. Ghamari, and S. Naghavi
Application of glutenin macro-polymer (GMP) gel analysis compared to conventional wheat quality indicators such as total protein content, Zeleny, and SDS sedimentation values was evaluated in quality classification of 13 Iranian wheat cultivars. The results showed no significant correlation between total protein content and breadmaking characteristics. Zeleny, SDS sedimentation and GMP tests showed significant correlation with loaf volume and bread height. GMP wet weight and small-strain deformation rheological characteristic of GMP-gel were correlated with large-strain deformation rheological properties of dough measured in Farinograph and bread quality (loaf volume and height). Significant (α < 0.01) correlation was found between rheological properties of the GMP gel and Farinograph characteristics of dough. Although GMP wet weight is regarded as a predictive measure for breadmaking quality of wheat, in the light of the results of this rather small sample set we did not find significant correlation between small-strain rheological properties of GMP-gel (storage modulus and tan δ) and breadmaking characteristics.
Authors:Gustavo Guadagnucci Fontanari, José Manuel Martins, Marcelo Kobelnik, Iêda Aparecida Pastre, José Alfredo Gomes Arêas, José Paschoal Batistuti, and Fernando Luis Fertonani
Different methods of preparing PIs were tested, resulting in final products that were different only in relation to the yield and protein content. The results of the proteinanalysis by SDS-PAGE showed that the same protein fractions were
Authors:G. Balázs, S. Tömösközi, A. Harasztos, V. Németh, Á. Tamás, A. Morgounov, I. Belan, W. Ma, and F. Békés
Based on previous research on validating lab-on-a-chip data on wheat protein analysis, a comprehensive work has been carried out with the intent to demonstrate the potential of the technique for wheat related fundamental research, breeding and food industry. Sample preparation and separation methodologies were investigated for the main wheat polypeptide classes: albumins, globulins, gliadins and glutenin subunits (GS). The work was carried out on a sample population originated from Western Siberia with different genetic background providing data, and characterizing their potential interest for future breeding work. LOC results are compared with corresponding reference methods (MALDI-TOF and RP-HPLC). The research revealed that, the current technology is capable for fast profile analysis, recognizing the minor qualitative, and typical quantitative differences in the albumin and globulin protein composition. While the gliadin separation showed poor results, the method seems to be able to identify the high molecular glutenin allelic composition, and to differentiate some of the low molecular weight glutenin alleles, too. Our results provide new insights into a possible rapid and simple way for grain protein profiling.
High temperatures during seedling growth are considered as one of the factors that can modify surviving properties in wheat (
L.) plant. This work attempts to evaluate the heat shock responses of seedling of winter wheat (Bezostaya-1) using growth parameters (seedling length, embryonal root length and embryonal root number), membrane stability index (MSI) and two dimensional (2D) gel electrophoresis analysis of heat shock proteins (HSPs) during heat shock. Seedlings grown until first leaf opening at controlled conditions (23 °C, 200 μmol m
, 16h day/8h night, 50–60% humidity) were exposed to 37 °C or 45 °C high temperatures for 2, 4 and 8 hours. While 37 °C did not cause any significant change, 45 °C heat treatments caused significant decrease in terms of seedling and root length, and leaf MSI for all exposure times. However, all the plants from 45 °C heat treatments continued to grow during recovery period. 2D protein analysis indicated that 37 °C, 8 hours exposure caused stronger and more diverse heat shock response than the other treatments, followed by 37 °C, 4 hours, 45 °C, 8 hours, 45 °C, 4 hours, 45 °C, 2 hours treatments. 5 protein spots, ranging from 6–7.8 p
(isoelectric point) and 27–31.7 kDA molecular weight, were expressed at 37 °C, 2 hours and continued at 37 and 45 °C for all exposure times. This suggests that these early proteins and other newly synthesized proteins may have protective effects at 37 and 45 °C and provide plants for healthy growth during the recovery period.
Authors:A. Çelebi, M. Tekşen, L. Açİk, and Z. Aytaç
L. is one of the examples from the several unsolved taxonomic problems. The classical identification of plant depends on morphological characteristics. Therefore, it is difficult to determine the botanical origin of some plants. The goals of this study were to examine the taxonomic status of these 42 taxa of
in Turkey by means of RAPD-PCR and seed protein analysis in addition to taxonomic interpretation of species. SDS-PAGE and RAPD-PCR techniques were used to help taxonomic interpretation of
species. Dendrogram based on
enetic distances was calculated using the computer programmed PopGen. For the RAPD-PCR analysis, 40 random primers were tested in the amplification reactions with
species. Only 9 of them gave consistently reproducible banding patterns. The analysis of seed proteins showed that all studied genotypes had a specific protein pattern except the species that were close to each other based on morphological data. In electrophoretic protein banding patterns a total of 22–30 protein bands were observed. The protein bands in the region between 116-66 kDa were almost similar in all the species tested. In addition to that, all the species of
had two bands in common (just bigger than 25 kDa).
are very close relatives according to morphological, RAPD and protein results. Therefore these two taxa can be considered as synonyms.
are grouped together. All these separate species treated as synonyms based on morphological and molecular data.
Authors:A. Farsang, L. Makranszki, M. Dobos-Kovács, Györgyi Virág, Katalin Fábián, Tímea Barna, G. Kulcsár, L. Kucsera, and F. Vetési
An outbreak of the atypical form of myxomatosis struck a rabbit farm in Hungary. The animals had previously been vaccinated with a vaccine containing Shope rabbit fibroma virus strain. The disease appeared in winter when the presence of mosquitoes and fleas is not common. The virus was isolated from an eyelid specimen of a naturally infected rabbit. The surviving animals were observed for four weeks, blood samples were collected and, after euthanasia, organ specimens were also examined by morphological methods including pathology and electron microscopy. Serum samples were examined by virus neutralisation for antibodies. Genetic analysis of the isolated virus was carried out by polymerase chain reaction (PCR) and direct sequencing. The primers were designed on the basis of the major envelope gene (Env) of the Lausanne reference strain in the GenBank. The viral proteins were examined by SDS-PAGE. The isolated virus (ref. no.: BP04/2001) was able to infect the susceptible animals directly, by contact. The disease was characterised by respiratory symptoms of the upper tracheal tract, conjunctivitis and high mortality by the 11th-14th day. Aerogenic infection with strain BP04/2001 resulted in 100% morbidity among the susceptible animals. Sequencing of the amplified 400-bp-long DNA revealed 97% homology with the Env gene of the Lausanne strain, which proves that strain BP04/2001 is a variant of the Lausanne strain having been enzootic throughout Europe. The live vaccine strain used in Hungary against myxomatosis, which is also a Lausanne-derived strain, protected the animals. According to the protein analysis a protein of 200 kDa in size is not expressed in strain BP04/2001. This is the first report on atypical myxomatosis in Central Europe. The virus spreads by airborne transmission and may cause severe losses in the rabbit population.
Authors:Annamária Ágota, Bence Ágg, Kálmán Benke, József Gábor Joó, Zoltán Langmár, Krisztina Marosi, Zsuzsanna Lelovics, Kitti Deé, Péter Nagy, Bernadett Köles, Endre Horváth, Zsuzsanna Crespo, Zoltán Szabolcs, and Zsolt B. Nagy
A Marfan-szindróma a szervezet kötőszöveti állományát érintő öröklődő betegség, amely Magyarországon hozzávetőleg 2–3000 személyt érint. A betegség manifesztációi multiszisztémásak, ezért a kórismézés sokszor nehézségekbe ütközik. Az „Országos Marfan Regiszter” jelenleg közel 250 Marfan-szindrómában szenvedő beteg adatait tartalmazza, s ez a szám dinamikusan növekszik. Célok: A Marfan-szindrómás magyar személyek biológiai mintáinak, klinikai adatainak és életmódfelmérőinek összegyűjtése. Módszerek: A szelekció során olyan betegekre esett a választás, akiknél a cardiovascularis és a szisztémás érintettség, illetve a családi kórelőzmény alapján az átdolgozott Gent-nozológia értelmében egyértelműen megállapítható a Marfan-szindróma fennállása. Eredmények: Az adatbázis alapján 102 Marfan-szindrómában szenvedő személyt tartalmaz a biobank, amely 55 személy cDNS-mintáját, 102 személy genomi DNS- és szérummintáját foglalja magába. A biológiai minták mellett minden személyről nemzetközileg validált fizikai aktivitási, táplálkozási és pszichológiai kérdőívek alapján gyűjtött adatot is tartalmaz a biobank. Következtetések: A Marfan Biobank genetikai, génexpressziós és fehérjeszintű kutatások gyors megtervezését teszi lehetővé. A biobank hozzájárul a kórkép alaposabb megismeréséhez és a hazai betegek ellátásához egyaránt. Orv. Hetil., 2012, 153, 296–302.