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Acta Veterinaria Hungarica
Authors: Orsolya Erdősi, Katalin Szakmár, Olivér Reichart, Zsuzsanna Szili, Noémi László, Péter Székely Körmöczy, and Péter Laczay

505 515 O’Grady, J., Ruttledge, M., Sedano-Balbás, S., Smith, T. J., Barry, T. and Maher, M. (2009): Rapid detection of Listeria monocytogenes in food using culture enrichment

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Acta Alimentaria
Authors: O. Erdősi, K. Szakmár, O. Reichart, Zs. Szili, N. László, Z. Balogh, P. Székely Körmöczy, and P. Laczay

.C. & Solomon, M.B. (2006): Rapid detection of Salmonella from hydrodynamic pressure-treated poultry using molecular beacon real-time PCR. Fd Microbiol., 23 , 39–46 Solomon M.B. Rapid

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Acta Microbiologica et Immunologica Hungarica
Authors: Reza Ranjbar, Ali Naghoni, Shohreh Farshad, Hadi Lashini, Ali Najafi, Nourkhoda Sadeghifard, and Caterina Mammina

resistance fact sheet no. 139 2003 Karami, A., Ranjbar, R., Ahmadi, Z., Safiri, Z.: Rapid detection of different serovares of

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A real-time RT-PCR assay utilising light upon extension fluorogenic primer (LUX RT-PCR) was developed for the rapid and efficient detection of avian influenza viruses (AIV). The assay detected each of the AIV isolates tested (16/16) and gave negative results with heterologous pathogens (17/17). The detection limit of the assay proved to be 10-0.5 EID50/0.2 ml and 101.5 EID50/0.2 ml in allantoic fluid of virus-infected embryonated chicken eggs and in spiked chicken faeces samples, respectively. Based on its specificity, sensitivity and relative simplicity, the LUX RT-PCR assay provides a novel, rapid and cost-effective diagnostic tool for avian influenza surveillance and monitoring programs.

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Acta Veterinaria Hungarica
Authors: Dorottya Földi, Zsuzsa Kreizinger, Katinka Bekő, Nikolett Belecz, Krisztián Bányai, Krisztián Kiss, Imre Biksi, and Miklós Gyuranecz

Gyuranecz , M. ( 2018 ): Development of molecular methods for the rapid detection of antibiotic susceptibility of Mycoplasma bovis . Vet. Microbiol. 213 , 47 – 57 . 10.1016/j.vetmic.2017.11.026 Sulyok , K. M. , Kreizinger , Z. , Wehmann , E

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Fusarium head blight disease (FHB) in wheat, caused by Fusarium graminearum species complex (Fg complex), is a very serious disease threatening wheat production worldwide. Polymerase chain reaction (PCR) based methods have been established for rapid and quantitative detection of many plant pathogens. In this study, a specific pair of primers was designed based on the sequence of DNA fragment (740 bp) amplified by a microsatellite primer M13 from Fg complex isolates. This pair of primers was able to amplify a 380 bp fragment from all Fg complex isolates but not from any other tested fungal species. Using this pair of primers, a real-time PCR assay was developed to quantitatively detect small amounts of Fg complex in wheat seeds. This sensitive and quantitative detection assay could be useful in epidemiological studies and assessment of mycotoxin contamination in wheat seeds.

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Acta Microbiologica et Immunologica Hungarica
Authors: Fei Zhao, Zhong Liu, Yixin Gu, Yuelian Yang, Di Xiao, Xiaoxia Tao, Fanliang Meng, Lihua He, and Jianzhong Zhang

27 2492 2496 Curtis, K. A., Rudolph, D. L., Owen, S. M.: Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification

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Notzon, A., Helmuth, R. and Bauer, J. (2006): Evaluation of an immunomagnetic separation-real-time PCR assay for the rapid detection of Salmonella in meat. J. Food Prot. 69 , 2896–2901. Bauer J

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R.N. Bryan 1999 Rapid detection of the mec A gene inmethicillin resistant staphylococci using a colorimetric cycling probe technology Diagn Microbiol Infect Dis

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