Authors:Yunfang Zhou, Bingbao Chen, Junyan Chen, Yanwen Dong, Shuanghu Wang, Congcong Wen, Xianqin Wang and Xiaomin Yu
chromatography–tandem mass spectrometric (LC–MS/MS) method developed for the quantification of jaceosidin in ratplasma to characterize the pharmacokinetics of jaceosidin [ 21 ]. Song et al. developed a rapid, sensitive, and selective liquid chromatography
Authors:Peiwu Geng, Xinhua Luo, Xiufa Peng, Zixia Lin, Wenhao Chen, Jin Zhang, Congcong Wen, Lufeng Hu and Siyi Hu
, the object of current study was to establish a sensitive UPLC–MS/MS method for quantitation of eupatilin in ratplasma for the first time and apply it in the investigation of the pharmacokinetic properties of eupatilin.
Authors:Y. C. Xiao, L. T. Liu, J. J. Bian, C. Q. Yan, L. Ye, M. X. Zhao, Q. S. Huang, W. Wang, K. Liang, Z. F. Shi and X. Ke
profile in ratplasma after oral administration of SGJY. Moreover, the result of this study was expected to provide helpful chemical information for further pharmacology and active mechanism research on SGJY formula.
experiments, might be afforded by a diversity of bioactive compounds after oral administration, the pharmacokinetic features of the bioactive compounds need to be evaluated.
Several pharmacokinetic studies of atractylenolides in ratplasma samples
Authors:Mona Al-Shehri, Mohamed Hefnawy, Hatem Abuelizz and Adeeba Alzamil
-HPLC-PDA method with the selectivity and sensitivity needed to determine low concentrations of LTZ and PLB combination in ratplasma. After development and validation, the method will be applied to investigate the pharmacokinetics of LTZ and PLB in rats treated
Authors:C. Wen, Q. Zhang, Y. He, M. Deng, X. Wang and J. Ma
A sensitive and simple liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for determination of dasatinib in rat plasma using one-step protein precipitation was developed. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with methanol-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 488.2 for dasatinib and m/z 338.7 for the IS. Calibration plots were linear over the range of 10–1000 ng mL−1 for dasatinib in rat plasma. Lower limit of quantification (LLOQ) for dasatinib was 10 ng mL−1. Mean recovery of dasatinib from plasma was in the range 82.2%–93.6%. Relative standard deviation (RSD) of intra-day and inter-day precision were both less than 8%. This developed method is successfully used in pharmacokinetic study of dasatinib in rats.
Authors:K. Sakthimanigandan, M. Ganesh, V. Kanthikiran, T. Sivakumar and H. Jang
A new sensitive and validated liquid chromatography electro spray ion-tandem mass spectrometry (HPLC-ESI-MS/MS) method for the quantification of Vildagliptin (VG) in rat plasma has been developed and validated using repaglinide (RG) as an internal standard (IS). The analytes were extracted by liquid-liquid extraction using ethyl acetate. Elution of the VG and IS was achieved on a reverse phase Betasil (C18 50 mm 4.6 mm ID, 5 μ) column with an isocratic mobile phase composed of acetonitrile: 2 mM ammonium acetate (90:10 v/v). The analytes monitored in the Multiple Reaction Monitoring (MRM) mode were m/z 304.2→154.0 and 453.3→230.3 for VG and RG, respectively. The calibration curve was linear in the range of 1.57–501.21 ng/mL for VG with lower limit of quantification 1.57 ng/mL. The intra run and inter run precision values are within 11.70% for VG at LOQ level.
Authors:P.R. Ravi, S. Joseph, U. S. R. Avula and S. Anthireddy
The objective of this study was to develop and validate a novel, simple, and selective high-performance liquid chromatographic (HPLC) method with photodiode array detector for the estimation of tenofovir in rat plasma, which can be utilized in analyzing the pharmacokinetic samples from rats. Prior to analysis, an optimized protein precipitation technique was used to extract tenofovir from plasma. The mobile phase for this method comprised of 10 mM ammonium acetate buffer (pH 4) and methanol in the ratio of 97:3 υ/υ. Chromatographic separation of tenofovir was achieved using Spincotech C-18G enabled column (250 × 4.6 mm, 5 μm). Tenofovir was monitored at a wavelength of 260 nm, and the calibration curve was linear in the range of 250–4000 ng mL−1 (R2 = 0.999). High recovery obtained after extraction (97%–101%) of plasma samples precluded the use of an internal standard. Validation studies were performed as per the standard guidelines, and the developed method was accurate, precise, and selective for the determination of tenofovir in the rat plasma. The stability studies performed during the sample pretreatment process and sample storage conditions did not show a quantifiable degradation of tenofovir. Further, this method was able to estimate tenofovir and determine its pharmacokinetic parameters, post IV bolus administration in male Wistar rats. The pharmacokinetic profile of tenofovir followed one compartmental open model.
Authors:Peiwu Geng, Jing Zhang, Bingbao Chen, Qianqian Wang, Shuanghu Wang and Congcong Wen
quantitation of 10 alkaloids (daurisoline, dauricine, N -desmethyl daurisoline, dauricicoline, dauriporphinoline, bianfugecine, dauricoside, stepholidine, acutumine, and acutumidine) from Rhizoma Menispermi in ratplasma, and the validated method was
Authors:Meifei Lu, Xiaojie Lu, Zheng Yu and Congcong Wen
UPLC-MS/MS method was developed for determination of calycanthine in ratplasma, and pharmacokinetics in rats was investigated. Materials and methods Chemical and animals Calycanthine (purity > 98%, Fig. 1A ) and midazolam (purity > 98%, Fig. 1B