In this paper, several small-scale screening test methods were discussed on evaluating the thermal hazard of reactive substances.
Generally the sensitivities of DSC and ARC are not high enough to evaluate the thermal hazards for all reactive substance,
especially, for those of complex reactions containing a phase and/or chemical reaction mechanism change in the lower temperature
range. Using the C80, however, the reaction can easily be detected in the lower temperature range due to its high sensitivity.
Therefore, the C80 gives generally more accurate results than DSC and ARC. Data from C80 and Dewar vessel were compared and
it indicates that the Dewar vessel has also high enough sensitivity to evaluate the thermal hazard and determine the heat
flux in lower temperature range of reactive substances.
Authors:S. T. Balthazary, H.-P. Sallmann, and H. Fuhrmann
This study investigated the inhibitory efficiency of all-rac-α-tocopherol, 2,6- ditert-butyl-p-cresole (BHT), and 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carbonic acid (Trolox®) on determination of thiobarbituric acid-reactive substances (TBARS) and short-chain alkenals in rat liver homogenates. The concentration of TBARS was measured fluorophotometrically. Aldehydes were determined after derivatization with methylhydrazine by gas chromatography (GLC). The concentrations of alkenals and TBARS in liver homogenates were diminished when antioxidants were present dur- ing the sample preparation. It is suggested that in the absence of antioxidants the samples are autoxidized further during the preparative procedures. For the aldehyde determination all-rac-α-tocopherol was the most effective antioxidant to reduce the bias due to autoxidation, whereas for TBARS it was Trolox.
Authors:Celestyna Mila-Kierzenkowska, Alina Woźniak, B. Woźniak, G. Drewa, Bogumiła Chęsy, T. Drewa, Ewa Krzyżyńska-Malinowska, and R. Ceraficki
In this study we examined superoxide dismutase (SOD) activity and thiobarbituric acid reactive substances (TBARS) concentration in liver and muscles of four fish species: the carp, the brown trout, the white cod and the flounder. Higher SOD activity and higher TBARS concentration was revealed in the tissues of marine fish in comparison to freshwater fish. The highest SOD activity was observed in the cod while the highest TBARS concentration was in the flounder. The observed differences are probably an effect of the different living mode of the compared fish.
Lipid peroxide level in heart was determined as thiobarbituric acid reactivesubstances (TBARs) by the methods of Ohkawa et al. ( 24 ). The amount of lipid peroxides was measured as the production of MDA, which in combination with TBA forms a pink
Authors:E Özkök, H Yorulmaz, G Ateş, I Aydın, M Ergüven, and Ş Tamer
acid reactivesubstances (TBARS) as end products of lipid peroxidation, HIF-1α, and ghrelin in kidney tissue during early-phase sepsis.
Materials and Methods
This study was conducted at
Authors:M Stojanovic, V Zivkovic, I Srejovic, V Jakovljevic, N Jeremic, and D Djuric
last minute of perfusion with any of the test compounds. Oxidative stress parameters [the index of lipid peroxidation (thiobarbituric acid reactivesubstances, TBARS), the superoxide anion radical (O 2 •− ), H 2 O 2 , and nitrite (NO 2 − )] were
acid reactivesubstances (TBARS) method ( 53 ). Moreover, total antioxidant capacity (TAC) was measured according to a published method ( 20 ). The results are expressed as trolox equivalents per gram wet tissue weight. The levels of glutathione (GSH
Authors:CC Callegaro, AD Hoffmeister, FG Porto, L Chaves, RC Horn, AC Tissiani, PDA Bianchi, and JA Taylor
leads to oxidative damage ( 28 ). Oxidative stress can be directly evaluated through free radical production ( 21 ) or indirectly via end products of oxidative damage to lipids ( 9 ) and proteins ( 18 ). For example, thiobarbituric acid reactive
Authors:Y. Sakuma, K. Matsuoka, C. Honda, K. Matsumoto, and K. Endo
The dynamics of redox related elements (Fe, Co, Zn, and Se) were studied using instrumental neutron activation analysis as
a function of rat age in the range of 4 to 16 weeks. Activity levels of glutathione peroxidase (GSH-Px), thiobarbituric acid
reactive substance (TBARS) were assayed, and hydrogen peroxide (H2O2) concentrations were measured for the same liver homogenates using an X-band ESR spectrometer. The oxidative stress, the
aging effect, and the mineral valance are discussed.