Authors:Hyo Seon Kim, A Yeong Lee, Byeong Cheol Moon, Wook Jin Kim, and Goya Choi
interactions among different factors [ 10 ]. To overcome these shortcomings, Box and Wilson suggested the use of responsesurfacemethodology (RSM) [ 11 ]. RSM is an effective and efficient mathematical statistics method to construct models, evaluate the
Authors:Harleen Kaur, Amarjeet Kaur, Harvinder Saini, and Bhupinder Chadha
Lopez, J. L., Sanchez Perez, J. A., Fernandez Sevilla, J. M., Acien Fernandez, F. G., Molina Grima, E., Em Chisti, Y.: Fermentation optimization for the production by Aspergillus terreus : Use of responsesurfacemethodology. J. Chem. Technol
Authors:G. Banerjee, A. Ray, S.K. Das, R. Kumar, and A.K. Ray
Present study demonstrated the isolation of most promising β-galactosidase producing bacterial strain SB from soil. Morphological, biochemical, and 16s rRNA sequence analysis identified the bacterial strain as Arthrobacter oxydans. Several chemicals, including SDS, Triton X-100, Tween 20, isoamyl alcohol, and toluene-acetone mixture, were applied for extraction of intracellular β-galactosidase from the bacterial strain Arthrobacter oxydans. Among these, Tween 20 was recorded to be most effective. Role of pH, temperature, and shaker speed on production of β-galactosidase was evaluated using Box-Behnken design of response surface methodology. According to Box-Behnken analysis, optimum production of β-galactosidase (21.38 U (mg–1 protein)) is predicted at pH 6.76, temperature 36.1 °C, and shaker speed 121.37 r.p.m. The parameters are validated with the nearest value.