Five varieties of common buckwheat (
Moench) were tested in field experiments for three years. The aim of the study was to investigate the influence of the variety and environmental conditions on rutin distribution in the plant. The rutin content was determined by micellar electrokinetic capillary chromatography in stems, leaves and flowers at flowering stage and in achenes at maturity. The selected varieties differed statistical significantly in the rutin content in leaves, stems and achenes. Krupinka was the variety with the highest rutin content in the aboveground part of a plant. The tetraploid variety did not contained higher rutin level than diploid varieties. Krupinka had the highest rutin content in leaves contrary to the other varieties, which had the highest rutin content in flowers. Weather conditions influenced the rutin content in flowers, stems and achenes. The plant part with the highest rutin content can be different in diverse years.