Authors:J.R. Li, D.X. Li, L. Li, W.L. Deng, L.S. Ding, H.X. Xu, and Y. Zhou
A rapid and sensitive ultraperformance liquid chromatography-multiple reaction monitoring-multi-stage/mass spectrometry (UPLC-MRM-MS/MS) method has been developed for simultaneous quantification of salvianolic acid B and tanshinone IIA of salvia tropolone tablets in dog plasma. This was achieved by performing quantification using the MRM acquisition with two channels of MRM-MS/MS and MS full scan for more accuracy qualitative results, and the fragmentation transitions of m/z 295→249, 191 for tanshinone IIA and m/z 297→279, 251 for IS in positive mode, m/z 717→519, 321 for salvianolic acid B and m/z 295→267, 239 for IS in negative mode were selected. The UPLC separation was achieved within 3 min in a single UPLC run. Linear calibration curves were obtained over the concentration range of 10 pg/mL−1 ng/mL for tanshinone IIA and 100 pg/mL−1 for salvianolic acid B. Lower limit of quantitation (LLOQ) was 10 pg/mL and 100 pg/mL for tanshinone IIA and salvianolic acid B, respectively. The inter-day and intra-day precision (relative standard deviation, RSD) in all samples were less than 8.21%, and the recoveries were over 85.9% for both tanshinone IIA and salvianolic acid B. The two channels of MRM with MS full scan approach could provide both qualitative and quantitative results without the need for repetitive analyses and resulted in the reduction of further confirmation experiments and analytical time. The pharmacokinetic study of the two active components of salvia tropolone tablets following oral gavage administration of dogs was thus explored with this method.
Authors:W. Xiong, R.Q. Yan, Y.N. Liu, S.W. Peng, Z.Z. Jiang, X. Chai, A.D. Qi, and Y.F. Wang
Compound danshen preparations (CDPs) are used clinically for the treatment of cardiovascular and cerebrovascular diseases. By using the quantitative analysis of multi-components by single-marker (QAMS) method, sixteen compounds (danshensu, protocatechuic acid, protocatechuicaldehyde, caffeic acid, rosmarinic acid, lithospermic acid, notoginsenoside R1, salvianolic acid B, ginsenoside Rg1, ginsenoside Re, salvianolic acid A, salvianolic acid C, ginsenoside Rb1, ginsenoside Rd, cryptotanshinone, and tanshinone IIA were quantified on an ACQUITY ultraperformance liquid chromatography (UPLC) HSS T3 column (2.1 × 100 mm, 1.8 μm) with the mobile phase consisting of 0.1% formic acid aqueous solution (A) and acetonitrile (B) using a gradient elution at the flow rate of 0.30 mL/min in 30 min at 30°C, which was also validated by UPLC-diode array detection (DAD) and UPLC-electrospray ionization multistage/mass spectrometry (ESI-MS/MS) for assuring the feasibility and accuracy. Tested by robustness experiment under slightly changeable conditions, the stability of relative correction factor (RCF) proved to be stable, with RSDs below 5.69%, except for notoginsenoside R1 with relative standard deviation (RSD) 7.83%. This reliable and convenient QAMS method resolved the problem of standard substance insufficiency and improved the quality assessment of preparations consisting of complex compounds with different chemical structures, such as CDPs.
Authors:Long Wang, Yuan-Yuan Jiang, Li Zhang, Tao Wang, Rui-Wu Yang, Chun-Bang Ding, Xiao-Li Wang, and Yong-Hong Zhou
A high-performance liquid chromatography (HPLC) method has been developed for the simultaneous identification and quantification of active compounds (cryptotanshinone, dihydrotanshinone I, tanshinone IIA, tanshinone I, salvianolic acid A, salvianolic acid B, protocatechuic aldehyde, and rosmarinic acid) contained in traditional Chinese folk medicine Salvia przewalskii Maxim. The herb samples (including wild, cultivated, and yin pian) from fourteen main regions were investigated. Chromatographic separation was performed on an Agilent Eclipse XDB-C18 reserved-phase column (250 mm × 4.6 mm i.d., 5 μm) using gradient elution with water-formic acid (99.9: 0.1, v/v) and acetonitrile at a flow rate of 0.8 mL min−1, an operating temperature of 30 °C, and a wavelength of 275 nm. Similarity analysis (SA), principal component analysis (PCA), and hierarchical cluster analysis (HCA) were used to analyze the data based on fingerprints. For fingerprint analysis, 27 peaks were selected as the common peaks to evaluate the similarities among different samples. The results of SA showed that the method permits to obtain desired linearity, precision, accuracy, and recovery. All samples were divided into three categories by PCA and HCA, and the concentration of the eight bioactive compounds varied significantly from different regions. It was demonstrated that chromatographic fingerprinting by HPLC combined with the simultaneous determination of eight bioactive compounds was a helpful method for the quality control of S. przewalskii.