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to antibiotics. Additionally, the definition is necessary to enable the collection of epidemiological data. In this study, the polymerase chain reaction (PCR)-restriction enzyme analysis (PRA) method and DNA sequence analysis method were used to
Avian leukosis virus subgroup J (ALV-J) can cause a variety of neoplasms, including mainly myeloid leukosis (myelocytomatosis) and nephromas. Other tumours, such as histiocytic sarcoma (HS), haemangiosarcoma and mesothelioma, may also develop. In a previous article we described a case in which myeloid leukosis, haemangiomas and leiomyosarcomas appeared simultaneously in a commercial layer flock with infection by ALV-J. The present research was completed to understand the molecular characteristics of the ALV-J strain that induced clinical myeloid leukosis, haemangiomas and leiomyosarcomas. Two strains of ALV-J (SDAU1001 and SDAU1002) were isolated and identified, and their full-length sequences were analysed. The complete genome nucleotide sequences of these two isolates were different in length, 7652 nt and 7636 nt, respectively. They shared 98.9% identity with each other, and 93.4% to 97.8% nucleotide identity to the reference ALV-J isolates. A 19-nucleotide repeat sequence was identified in the primer binding site (PBS) leader region of isolate SDAU1001. A base substitution mutation (base 15 C-T) in this insertion was identified. However, the identical insertion at the same site was not found in SDAU1002. The gag and pol genes of the two viruses were more conserved than the env gene. One key deletion in the E element was a common feature of SDAU1001 and SDAU1002. SDAU1001 and SDAU1002, possibly recombinants of ALV-J and another avian retrovirus, may share the same ancestor. Co-infection by SDAU1001 and SDAU1002 isolates is a possible explanation why myeloid leukosis, haemangiomas, and leiomyosarcomas appeared simultaneously in the same commercial layer flock.
254 9839 9845 Ridder, R., Osiewacz, H., D.: Sequence analysis of the gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of Podospora
Irodalom/References 1 Abbott, A. (1995) Sequence Analysis. New Methods for Old Ideas. Annual Review of Sociology, Vol. 21. pp. 93
amplification from both corneal epithelium and fluid from contact lens storage was succesfull. Thereafter, the Acanthamoeba positive samples, detected by PCR method, were sequenced to identify the species. Sequence analysis using a BLAST search indicated an
cases. The samples for seven Acanthamoeba -positive patients, detected by PCR method, were sequenced to identify the species. Sequence analysis using a BLAST search indicated an identity of >98% with Acanthamoeba 18S rRNA gene reference sequences. It
The aim of the present study was to detect and characterise the species and subspecies of Babesia spp. that cause canine babesiosis in Croatia. Twenty-eight dogs with typical signs of babesiosis (lethargy, anorexia, fever, dark urine and thrombocytopenia) were included in this study. Their blood smears showed the presence of Babesia canis . The results showed the detection of one subspecies, namely Babesia canis canis using PCR, and subsequent sequence analysis demonstrated portions of the nss rRNA gene in 27 out of 28 samples. Sequence analysis of the isolates showed 100% identity in 11 samples, 99.7% identity (one nucleotide difference) in 11 samples and 99.4% identity (two nucleotides difference) in 5 samples with B. canis canis . The results of this study confirm the presence of B. canis canis in infected dogs in Croatia and demonstrate a slightly new genetic variant of Babesia subspecies.
In this study, the physical examination of 22 cattle revealed clinical signs of malignant catarrhal fever (MCF). Peripheral blood leukocyte (PBL) samples of the 22 cattle, and nasal (n = 7) and conjunctival (n = 9) swab samples from 16 sheep from two different farms, were taken for laboratory examination. The clinical diagnosis of MCF in cows was confirmed by the detection of ovine herpesvirus type 2 (OvHV-2) DNA by polymerase chain reaction (PCR). OvHV-2 DNA was detected by nested-PCR in PBL of one cow with clinical signs and nasal (1/7)-conjunctival(1/9) swab samples of two sheep housed in the same barn. According to the sequence analysis, three slightly divergent viruses were detected. The results indicate the need for additional research in different regions of Turkey to gain a better understanding of the incidence of MCF and its implications for the livestock industry.
. ( 1966 ): Isolation of an adenovirus from the brain of a pig . Am. J. Vet. Res. 27 , 751 – 758 . Kleiboeker , S. B. ( 1994 ): Sequence analysis of putative E3, pVIII
. Q. , Zuo , X. A. , Feng , J. and Su , N. ( 2013 ): ITS sequence analysis of Artemisia halodendron in different habitat gradients . – Sci. Cold Arid Reg. 5 ( 3 ): 347 – 352 . https://doi.org/10.3724/sp.j.1226
