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Acta Microbiologica et Immunologica Hungarica
Authors:
Károly Péter Sárvári
,
József Sóki
,
Miklós Iván
,
Cecilia Miszti
,
Krisztina Latkóczy
,
Szilvia Zsóka Melegh
, and
Edit Urbán

Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of these tests. The aim of this study was to determine the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method by testing of 400 Hungarian Bacteroides clinical isolates. Inaccurate identification results with MALDI-TOF MS were confirmed by 16S rRNA gene sequencing and findings were compared with traditional-automated biochemical test rapid ID 32A method as well.

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Lander, E. S., Linton, L. M., Birren, B. és mtsai: Initial sequencing and analysis of the human genome. Nature, 2001, 409 , 860–921. Birren B

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and catalase positive. The gram stain revealed gram negative cocci. 16S rRNA sequencing was performed for further investigation. DNA of the isolates was extracted using the boiling method. The amplification of the 911 bp location of the 16S rRNA gene

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1 Introduction Sequencing Batch Reactor (SBR) gained popularity in wastewater treatment since it is a single tank design, flexible system, with relatively easy automation [ 1 ]. The system configuration is quite simple; the tank has an inlet and

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Acta Veterinaria Hungarica
Authors:
Rinile Wu
,
Caifeng Yue
,
Yina Xie
,
Yuerong Fu
,
Xin Yu
,
Zhuoyuan Li
, and
Bin Yang

milk were extracted by an exosome purification kit (Umibio, Science and Technology Group, Shanghai, China). Four sRNA libraries were constructed, and sequenced on a HiSeq 2,500 sequencing system (Illumina, San Diego, CA, USA) with 50-bp single-end reads

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/ml ethidium bromide, and subsequently visualized under UV light. Next-generation sequencing of plasmid Next-generation sequencing of plasmid was carried out commercially by Illumina MiseqTM (Thermo Fisher Scientific, Waltham

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causes of severe disease. Routinely generated nucleotide sequencing information about EVs from clinical samples is usually limited to short, partial genomic sequences of VP1 that enable identifying the genotype but have limited use for detailed genetic

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-independent techniques such as 16S ribosomal RNA (16S rRNA) sequencing [ 10–12 ]; nevertheless, the overall microbial profile of prosthetic implants remains unclear. This study aimed to identify the microbial composition of orthopedic implants from AL and PJI cases

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Környezetből származó mikrobiális nukleinsavminták vizsgálati lehetőségei

Examination possibilities of microbial nucleic acid samples derived from the environment

Orvosi Hetilap
Authors:
Ivett Kriszta Kerekes
,
Ádám Nagy
,
Ágnes Ősz
, and
Péter Zalka

metagenomics: from microbiology to DNA sequencing technologies and bioinformatics. Front Genet. 2015; 6: 348. 8 Jeong J, Mun S, Oh Y, et al. A qRT-PCR method capable of

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. Johnston , B. R. ( 1998 ): Sequencing Capital Account Liberalization and Financial Sector Reform . IMF Paper on Policy Analysis and Assessment 98. Krugman , P

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