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liquid media improves in vitro shoot multiplication in Nyctanthes arbor tristis L. Appl. Biochem. Biotechnol. 163 , 851–859. Aref I M Preconditioning of axillary buds in

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–8. 24. Siddique , I. , Bukhari , N. A. , Perveen , K. , Siddiqui , I. , Anis , M. ( 2013 ) Preculturing of nodal explants in thidiazuron supplemented liquid medium improves in vitro shoot

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In vitro shoot multiplication of Telekia speciosa (Schreb.) Baumg. was tested on media containing benzyladenine, benzyladenine riboside, kinetin, zeatin, meta-topolin or 2-isopentenyladenine in different concentrations. We observed the proliferation rate, the length of shoots, rate of callus formation, and the presence of the hyperhydricity. The highest proliferation rate (13.17) was obtained on medium supplemented with 5.0 μM benzyladenine, however, the leaves were hyperhydrated at this concentration of benzyladenine, therefore for shoot multiplication lower (1.0 μM BA) concentration of benzyladenine is suggested. The longest shoots were achieved using 1.0 μM 2-iP. At this treatment 100% rooting was found, therefore the stage of rooting is omissible using 1.0 μM 2-iP during the multiplication. This in vitro propagation protocol should be useful for conservation as well as mass propagation of this plant species.

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Dobránszki, J., Abdul-Kader, A., Magyar-Tábori, K., Jámbor-Benczúr, E., Bubán, T., Szalai, J., Lazányi, J. 2000b: Single and dual effects of different cytokinins on shoot multiplication of different apple scions

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Faisal, M., Ahmad, N., Anis, M. (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tiss. Organ Cult. 80 , 187–190. Anis M. Shoot multiplication in

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Genkov, T., Ivanova, I. (1995) Effect of cytokinin-active phenylurea derivatives on shoot multiplication, peroxidase and superoxide dismutase activities of in vitro cultured carnation. Bulg. J. of Plant Physiol. 21 , 73

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shoot multiplication and adventitious regeneration from leaf explants Plant Cell Tiss. Org. Cult. 2006 85 145 149

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multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol. Plant. 29, 233–238. Anis M. In vitro shoot multiplication and

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Siddique, I., Anis, M. (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia Vahl.: a medicinal plant. Acta Physiol. Plant. 29 , 233–238. Anis M

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multiplication and their post-effects on rooting of apple cv. Hùsvéti rozmaring. Int. J. Hortic. Sci. , 6 (4), 84-87. Influence of aromatic cytokinins on shoot multiplication and their post-effects on rooting of apple cv. Hùsvéti

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