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The purpose of this study was to investigate the effects of endophytic fungi from tartary buckwheat on the host sprout growth and functional metabolite production. Without obvious changes in the appearance of the sprouts, the exogenous fungal mycelia elicitors notably stimulated the sprout growth and rutin accumulation, and the stimulation effect was mainly depended on the mycelia elicitor species along with its treatment dose. Three endophytic fungi Fat6 (Bionectria pityrodes), Fat9 (Fusarium oxysporum) and Fat15 (Alternaria sp.) were screened to be the most effective candidates for promoting F. tataricum sprout growth and rutin production. With application of polysaccharide (PS, 150 mg/l) of endophyte Fat6, PS (200 mg/l) of endophyte Fat9, and PS (150 mg/l) of endophyte Fat15, the rutin yield was effectively increased to 47.89 mg/(100 sprouts), 45.85 mg/(100 sprouts) and 46.83 mg/(100 sprouts), respectively. That was about 1.5- to 1.6-fold compared to the control culture of 29.37 mg/(100 sprouts). Furthermore, the present study revealed that the biosynthesis of the functional flavonoid resulted from the stimulation of the phenylpropanoid pathway by mycelia polysaccharide treatments. Application of specific fungal elicitors could be an efficient strategy for improving the nutritional and functional quality of tartary buckwheat sprouts.

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associated with resistance to pre-harvest sprouting in wheat. Crop Sci. 33 :453–459. Tanksley S.D. RFLP analysis of genomic regions associated with resistance to pre-harvest sprouting

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Acta Alimentaria
Authors: Sz. Szabó, Zs. Németh, É. Polyák, I. Bátai, M. Kerényi, and M. Figler

., Jark , U., Kirchner , M., Koch , J., Krause , G., Luber , P., Rosner , B., Stark , K. & Kühne , M. (2011): German outbreak of Escherichia coli O104:H4 associated with sprouts. New England J. Medicine, 365 , 1763

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1 1167 Splittstoesser, D. F., Queale, D. T. & Andaloro, B. W. (1983): The microbiology of vegetable sprouts during commercial production. J. Fd Safety , 5 , 79

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, E. ( 2010 ): Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa, buckwheat and wheat as affected by sprouting and baking . Food Chem. , 119 , 770 – 778 . APEDA ( 2016 ): http

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Acta Alimentaria
Authors: W. Wiczkowski, D. Szawara-Nowak, T. Sawicki, J. Mitrus, Z. Kasprzykowski, and M. Horbowicz

– 278 . Janovska , D. , ŠToČKovÁ , L. & Stehno , Z . ( 2010 ): Evaluation of buckwheat sprouts as microgreens . Acta Agr. Slov. , 95 , 157

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adults. We hypothesized that a 40% substitution of semolina flour with sprouted chickpea flour (SCF40), or non-sprouted chickpea flour (non-SCF40), would increase the antioxidant capacity in vitro as measured by trolox equivalent antioxidant capacity

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, protein synthesis, and other metabolic process will be initiated. After a certain period, the embryo will emerge from the seed, signed with the appearance of radicle or hypocotyl in the surface of the seed. The sprout of the seed will emerge because of the

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Pre-harvest sprouting (PHS) in bread wheat (Triticum aestivum L.) is one of the major abiotic constraints influencing production of high quality grain. Selection for pre-harvest sprouting (PHS) resistance in bread wheat (Triticum aestivum L.) in early generations is difficult because it is expressed as a quantitatively inherited trait and subject to environmental effects. The objectives of this study were to validate a major quantitative trait locus (QTL) for PHS resistance on chromosome 4A in bread wheat and to isolate near-isogenic lines for this QTL using marker-assisted selection. A total of 60 Canadian wheat cultivars and experimental lines were screened with three SSR markers in a QTL region for PHS resistance. The SSR markers DuPw004, barc170 and wmc650 explained 67%, 75% and 60% of total variation in germination (%), respectively, among different wheat genotypes. Marker assisted back crossing with DuPw004 reduced the population size in BC1F1 and BC2F1 generation by 41% and 59%, respectively. A survey of pedigrees of different genotypes revealed that the parental line RL4137 is a major source of increased PHS resistance in a number of western Canadian wheat cultivars. Microsatellite markers (DuPw004, barc170 and wmc650) will be useful for plant breeders to pyramid QTL from different PHS resistance sources.

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The paper discusses a possibility to grow seeds on solutions of microelements and application of sprouts enriched in such a way as an alternative to commercial dietary supplements. It contains a short review of the approaches reported till now and a systematic experimental study, carried on the most frequently used seeds (Lens culinaris, Helianthus annuus, Vigna radiata, Glycine max, and Lepidium sativum).Seven metals (Fe, Cu, Zn, Ni, Co, Cd, and Mn) were studied. Seeds were grown on cellulose in 20°C temperature using deionized water enriched with metals in concentrations: 100, 50, 25, 12.5, 6.25, and 3.125 mg/L in a period of 4 days. The reference samples were the seeds grown on pure deionized water. Sprouts were mineralized by microwave radiation, and the metal content was quantified by ion chromatography with on-line post-column derivatization and spectrophotometric detection.The conclusions can be treated as general recommendations, which seeds should be grown and what concentrations of metals in solutions should be applied to provide good enrichment and to avoid risk of microelement overdose.

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