Authors:Z.Y. Yang, C.Y. Liu, Y.Y. Du, L. Chen, Y.F. Chen, and Y.G. Hu
Yang , Z. , Zheng , J. , Liu , C. , Wang , Y. , Condon , A.G. , Chen , Y. , Hu , Y.-G.
2015 . Effects of the GA-responsive dwarfing gene Rht18 from tetraploidwheat on agronomic traits of common wheat . Field Crops Res. 183
Authors:N. Daskalova, S. Doneva, Y. Stanoeva, I. Belchev, and P. Spetsov
Four amphiploid lines (SHW) based on T. monococcum (Tm) and T. boeoticum (Tb) were crossed to T. durum varieties to generate 13 combinations. Field germination and winter survival of hybrid plants in F2 were assessed. Among all crosses, those with SHW8A-Tb and SHW9A-Tm showed highest field germination but with different degrees of spike fragility. The variation on seed number and weight per main spike was studied in F4–6 from SHW8ATb/ Progres and SHW5A-Tb/Severina crosses after individual selection for these traits. Ten lines with durum phenotype from the former and three genotypes with dicoccum plant shape from the latter cross were developed. SDS-PAGE indicated the presence of HMW-GS 1Ax2*+1Aynull subunits in four lines, among which 1Ax2* was inherited from T. boeoticum acc.110 through SHW8A-Tb. Most of the selected genotypes possessed γ-gliadin45, which was relating to good end-use quality. Powdery mildew testing showed that all progenies resulted from the SHW8A-Tb/Progres were susceptible to 12 races of the pathogen, while three lines derived from the SHW5A-Tb/Severina cross behaved differently: G32 expressed resistance to six, G33 to 2, and G34 to 5 races. The selected genotypes from crosses involving SHW with T. boeoticum exhibited good breeding performance compared to tetraploid wheat parents, and might be of breeding interest to further research.
Authors:C. Riefolo, D. Ficco, L. Cattivelli, and P. Vita
Levy, A.A., Galili, G., Feldman, M. 1988. Polymorphism and genetic control of high molecular weight glutenin subunits in wild tetraploidwheat Triticum turgidum var. dicoccoides . Heredity 61 :63–72.
Authors:K. Dong, C. Hao, A. Wang, M. Cai, and Y. Yan
The wheat storage proteins, especially the high molecular weight glutenin subunits (HMW-GS), play important roles in the determination of flour processing and bread-making quality. Compared with the traditional SDS-PAGE method, reversed-phase high-performance liquid chromatography (RP-HPLC) was shown to have many advantages for the separation and characterization of HMW-GS because of its high resolving power, repeatability and automation. In this work, HMW-GS from bread and tetraploid wheats were separated and characterized by RP-HPLC. The elution time ranking of different HMW-GS was: 1Ax > 1Bx > 1Dx > 1By > 1Dy. Several subunit pairs associated with good quality properties and those with similar mobilities on SDS-PAGE, such as 1Bx7 and 1Bx7*, 1By8 and 1By8*, 1Dx2 and 1Ax2*, 1Bx6 and 1Bx6.1, were well separated and readily identified through RP-HPLC. However, other subunit pairs, such as 1Dy10 — 1Dy12, 1Dx5 — 1By18 and 1Dx2 — 1By16, could not be adequately separated and identified by RP-HPLC, whereas they displayed different mobilities on SDS-PAGE gels. Because 1Dx5 and 1Dx2 showed different hydrophobicities, RP-HPLC could distinguish 1Dx5 + 1Dy10 and 1Dx2 + 1Dy12. A comparative analysis between RP-HPLC and SDS-PAGE showed that a combination of both methods provided more effective identification of HMW-GS in wheat quality improvement and germplasm screening.
Authors:I. Bellil, O. Hamdi, A. Benbelkacem, and D. Khelifi
Wheat endosperm storage proteins are the major components of gluten. They play an important role in dough properties and in bread making quality in various wheat varieties. In the present study, the different alleles encoded at the 5 glutenin loci were identified from a set of 38 tetraploid wheat germplasm obtained from interspecific crosses between durum wheats (Triticum turgidum L. ssp. durum (Desf.) Husn.) and their relatives (T. dicoccum Schübl. and T. polonicum L.) using SDS-PAGE. At Glu-A1 and Glu-B1, encoding high molecular weight glutenin subunits (HMW-GS), 2 and 4 alleles were observed, respectively. Low molecular weight glutenin subunits (LMW-GS) displayed similar polymorphism, as 3, 5 and 3 alleles were identified at loci Glu-A3, Glu-B3 and Glu-B2, respectively. One new allele was detected at Glu-B3 locus and appeared in nine accessions obtained from five crosses. This allele codes for five subunits (2 + 8 + 9 + 13 + 18), encoded by the Glu-B3b without subunit 16 plus subunits 2 and 18. A total of 38 patterns resulted from the genetic combination of the alleles encoding at the five glutenin loci. This led to a significantly higher Nei coefficient of genetic variation in Glu-1, Glu-3 and Glu-B2 loci (0.54). The germplasm analyzed exhibited allelic variation in HMW and LMW glutenin subunit composition and the variation differed from that of tetraploid wheats of other countries. The presence of high quality alleles in glutenin loci have led the accessions to be considered as an asset in breeding programs aimed for wheat quality.