Anand, L., Krishnamurthy, S., Vithayathil, P. J. (1990) Purification and properties of xylanase from the thermophilicfungus, Humicola lanuginosa Griffon and Maublanc Bunce. Arch. Biochem. Biophys. 276 , 546
Ten different strains of
, isolated from composting soils were found to produce phytase when grown on PSM medium. The wild type strain CM was found to produce maximum amount of phytase (4.33 units/g DW substrate). Culturing
strain CM on medium containing wheat bran and optimizing other culture conditions (carbon source, media type, nitrogen source, level of nitrogen, temperature, pH, inoculum age, inoculum level and moisture), increased the phytase yield to 13.26 units/g substrate. This culture was further subjected to UV mutagenesis for developing phytase hyperproducing mutants. The mutant (TL-7) showed 2.29-fold increase in phytase activity as compared to the parental strain. Employing Box-Behnken factor factorial design of response surface methodology resulted in optimized phytase production (32.19 units/g of substrate) by mutant TL-7. A simple two-step purification (40.75-folds) of phytase from mutant TL-7 was achieved by anion exchange and gel filtration chromatography. The purified phytase (∼54 kDa) was characterized to be optimally active at pH 5.0 and temperature 70 °C, though the enzyme showed ∼70% activity over a wide pH and temperature range (2.0–10.0 and 30–90 °C, respectively). The phytase showed broad substrate specificity with activity against sodium phytate, ADP and riboflavin phosphate. The phytase from
was thermoacidstable as it showed up to 70% residual activity after exposure to 70 °C at pH 3.0 for 120 min. The enzyme showed K
4.55 μM and V
0.833 μM/min/mg against sodium phytate as substrate.
Authors:J. M. Rezessy-Szabó, E. Bujna, and Á. Hoschke
): Production of α-galactosidase by thermophilicfungus Humicola sp. in solid-state fermentation and its application in soyamilk hydrolysis. Process Biochem. , 33 , 337-343.
Production of α-galactosidase by thermophilicfungus
Authors:V. Jurkovich, E. Brydl, P. Rafai, and et al.
Non-starch polysaccharides (NSPs) form an integral part of the cell walls in plants and represent considerable available energy when degraded into absorbable mono-, di-, tri- and oligosaccharides. The ruminal microflora hydrolyses a good part of NSPs, however, recently there have been attempts to enhance the rate of utilisation by using external polysaccharidase enzymes. In the present study the effects of an enzyme preparation (Rumino-Zyme) high in xylanase activity were studied on ruminal volatile fatty acid (VFA) concentration, parameters of energy and protein metabolism, milk yield, feed conversion ratio (FCR) and body condition score of high-yielding dairy cows. A lignolytic enzyme preparation produced by the thermophilic fungus Thermomyces lanuginosus was applied in the present experiment and fed to dairy cows at 34 g/day dosage in the period between calving and the 110th day of lactation. This preparation increased VFA concentration in the rumen from about 32 days after calving and onward. Increased VFA concentration was followed by an about 5 to 10% increase in milk production and an almost 0.1% increase in butterfat production. Increased VFA concentration produced more balanced energy metabolism in the experimental cows as indicated by the lower incidence rate of hyperketonaemia, and lower acetoacetic acid and non-esterified fatty acid (NEFA) concentration in the blood of the experimental cows. Aspartate aminotransferase (AST) activity was tendentiously higher in the control group and the proportion of cows that had AST activity higher than 100U/l was also higher in the control group. Both control and experimental cows showed balanced protein and acid-base metabolism throughout the experiment. Enhanced VFA concentration contributed to an improvement in energy balance in the experimental cows with a resultant improvement of feed intake and feed utilisation. Due to the more balanced energy metabolism postparturient body condition loss of the treated cows was reduced.
Authors:J. Kutasi, Á. Bata, E. Brydl, P. Rafai, and V. Jurkovich
. , Purkarthofer , H. , Hayn , M. , Kapplmüller , J. , Sinner , M. and Steiner , W. ( 1993 b ): Production of a high level of cellulase-free xylanase by the thermophilicfungus Thermomyces lanuginosus in laboratory and pilot scales using
Authors:Abeer H. Ali, Usama Radwan, Soad El-Zayat, and Magdi A. El-Sayed
natural compounds ( Tan & Zou, 2001 ). For example, Curvularia sp. confers heat tolerance to its host plant Dichanthelium lanuginosum ( Redman et al., 2002 ). Thermomyces sp. is a thermophilicfungus that can survive at 62 °C ( Mchunu et al., 2013