Authors:Marzieh Aligholi, Mohammad Emaneini, Morovat Taherikalani, Shadi Shahsavan, Fereshteh Jabalameli, Parisa Asadollahi, Babak Khoramian, and Mohammad Eslampour
. R., Appelbaum, P. C.: Activities and time-kill studies of selected penicillins, beta-lactamase inhibitor combinations, and glycopeptides against Enterococcus faecalis . Antimicrob. Agents Chemother. 42 , 857–861 (1998
Authors:Péter Feiszt, György Schneider, and Levente Emődy
Time-kill studies and growth arrest conditions
Time-kill assays were performed by methods based on the CLSI standard [ 10 ]. Mueller–Hinton broth was used in 10 ml final volumes. Antibiotics were
Authors:Máté Szász, Mária Hajdú, Natasa Pesti, Mónika Domahidy, Katalin Kristóf, ákos Zahár, Károly Nagy, and Dóra Szabó
Biofilm-forming Staphylococcus epidermidis strains are common cause of the periprosthetic infection. The treatment of the periprosthetic infection is very problematic, so the prevention of these infections by an antibiotic containing prothesis could be an option for prevention.The purpose of the present study was to examine the in vitro effects of drug delivery systems (DDSs), namely Wax 1 and Wax 2 with different vancomycin content: 0.5, 1, 2 and 4 mg. In order to control the antibacterial activity of DDSs killing curve study was performed and in order to determine the antibiotic release and the antibiotic peak concentration from the DDSs biological assay was carried out.The time kill curve studies showed, that both DDSs with all vancomycin concentration decreased significantly the bacterial counts, however, Wax 2 with 4 mg vancomycin significantly decreased the bacterial count than all the other groups.The vancomycin release was the best with the highest peak concentration from DDSs with 4 mg vancomycin contain; it was significantly better than in the other groups, however, no significant difference was observed between Wax 1 and Wax 2 in this respect.These findings suggest that Wax 2 with 4 mg vancomycin content could be a potential agent for clinical use.
Susceptibility Tests for Bacteria that Grow Aerobically, 4th edition. Approved Standard-M7-A4 (1997).
Spangler, S. K., Jacobs, M. R., Appelbaum, P. C.: Time-kill studies on susceptibility of nine penicillin susceptible and
Authors:I. K. Bedaida, S.H.D. Masry, B. Mamache, M.G. Shehata, L. Benammar, and A. Ayachi
over 24 h intervals, placed on PCA (Plate Count Agar) plate in triplicate and incubated at 37 °C for 24 h. Time-kill curves were realised by plotting log 10 CFU ml –1 against time (h). 1.6 Bacteriolysis and nucleic leakage To study the integrity of
Authors:Samuel Füchtbauer, Soraya Mousavi, Stefan Bereswill, and Markus M. Heimesaat
within 24 hours of co-incubation. The authors measured an MIC of capsaicin against P. gingivalis of 16 mg/L and an MBC of 64 mg/L. Kinetic time-kill studies revealed that P. gingivalis growth was inhibited in a capsaicin concentration- and time