Authors:Sobiya Shafique, Shazia Shafique and Aqeel Ahmad
Pakistan holds the position of top chilies producers. So Capsicum annuum L. production in Pakistan should be promoted by combating against diseases. The only solution is to cultivate resistant varieties. Presently six chili varieties were treated with Fusarium oxysporum Schlecht. and screened for the most resistant and the most susceptible varieties. Representative varieties were evaluated for their biochemical and transcriptional profiles to discover the bases of antifungal-resistance. Results concluded that the most resistant variety was “Dandicut” and the most susceptible was “Ghotki”. Tannins, coumarins, flavonoids, phenolics, Riboflavins and saponins were observed in higher quantities in Dandicut as compared to Ghotki. Defense related enzymes i.e. polyphenol oxidase, phenyl ammonia lyase and peroxidase were found in elevated amounts in Dandicut than in Ghotki. Transcriptional results showed that defense related genes i.e. PR2a, acidic glucanase; Chitinase 3, acidic; Osmotin-like PR5 and Metallothionein 2b-like had higher expressional rates in Dandicut. Pearson’s correlation coefficient revealed stronger direct interaction in signal transduction and salicylic acid pathway. Resistance of chili varieties is salicylic acid based. Results obtained from this study not only help to improve chili production in Pakistan but also facilitate variety development operations. Moreover, it also constructed a scale to evaluate innate resistance among varieties.
Authors:Fotini Krania, Eleni Dovolou, Constantinos A. Rekkas, Sonia Heras, Ioannis Pappas, Ann Van Soom and Georgios S. Amiridis
. , Salilew , D. , Jennen , D. , Tholen , E. , Sirard , M. A. , Schellander , K. and Tesfaye , D. ( 2006 ): Large-scale transcriptionalanalysis of bovine embryo biopsies in relation to pregnancy success after transfer to recipients . Physiol
. D. , Gaeta , M. L. , Mariath , J. E. D. A. , Costan F. D. , Retallick , J. , Nolan , L, , Tai , H. H. , Stromvik , M. V. , Fett-Neto , A. G. ( 2015 ) Comparative transcriptionalanalysis provides new insights into the molecular basis