Authors:V. Pachauri, V. Mishra, P. Mishra, A.K. Singh, S. Singh, R. Singh and N.K. Singh
.K. 2010. Combining QTL mapping and transcriptomeprofiling of bulked RILs for identification of functional polymorphism for salt tolerance genes in rice ( Oryza sativa L.) Mol. Genet. Genomics 284 :121–136.
Authors:Carloalberto Petti, Khan Mojibur and Fiona Doohan
strain MKB 158, can inhibit the development of Fusarium seedling blight disease on wheat and barley. Application of this and other pseudomonads as head sprays inhibits the development of Fusarium head blight disease (FHB) disease on wheat and barley under both field and glasshouse conditions.
strains MKB 158 and MKB 249 also reduced DON contamination of grain under field conditions. Evidence suggests that
does not directly inhibit the growth of
, but that it potentiates host resistance against this disease. Transcriptome profiling identified barley genes differentially expressed as early events in (a) bacterium-induced resistance to seedling blight and (b) heads following
co-inoculation. Bacterium-potentiated resistance to
affects the transcription of many genes that are involved in diverse processes, including cell rescue and defence, metabolism, cell cycle and DNA replication and signalling.
Authors:A. Shoaib, A. Aldaoude, M.I.E. Arabi, E. Al-Shehadah and M. Jawhar
Scald (Rhynchosporium secalis; Rs) and spot blotch (Cochliobolus sativus; Cs) are important diseases of barley (Hordeum vulgare L.) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the barley defense response since both these pathogens are necrotrophic fungi. In the current study, the transcriptome in leaves of the same barley genotype WI2291 inoculated with Rs and Cs was compared at different times postinoculation. Comparison of data for barley Rs- and Cs- inoculated plants with mockinoculated plants revealed gene expression changes that included basal defense transcripts and transcripts specific to the establishment of a necrotrophic interaction with associated fungi. During barley–pathogen interaction pathway, WI2291 activated a higher number of genes and pathways in response to Rs infection than in response to Cs invasion. However, families of genes encoding pectin-degrading enzymes, secondary metabolism enzymes, transporters and peptidases are expanded to cover Rs and Cs at an early stage following inoculation. Our results demonstrate differences in the pathways and activated genes of barely cv. WI291challenged by Rs and Cs, and that expression patterns of the same defenseassociated genes were altered in adaptation to different pathogens. Our work provides new insights into the underlying mechanisms related to regulation of different pathways in response to fungal infection.
Authors:A. Aldaoude, A. Shoaib, E. Al-Shehadah, M. Rajeh, M. Jawhar and M.I.E. Arabi
Scald caused by Rhynchosporium secalis, is an economically important disease found worldwide. In order to profile genes and pathways responding to R. seclais infection, leaf transcriptomes before and after fungus inoculation in susceptible barley were compared using cDNA-AFLP technique. Transcriptional changes of 144 expressed sequence tags (ESTs) were observed, of which 18 have no previously described function. Functional annotation of the transcripts revealed a wide range of pathways including cell wall fortification, cytoskeleton construction and metabolic processes at different time points. Furthermore, the results of RT-PCR analysis on candidate genes, ABC transporters and lycine-specific demethylase were consistent with the cDNA-AFLP data in their expression patterns. Taken together, our data suggest that susceptible barley reprograms metabolic and biological processes to initiate a suitable response R. secalis infection.