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Abstract  

The interweaving of three different sorts of software based on different algorithms (co-world analysis and downward hierarchical classification) and applied on a file (in the field of risk assessment through the introduction of transgenic plants) extracted from the CAB (Commonwealth Agricultural Bureau) data base, has enable us to provide three types of results: Leximappe provides a synthetic image from clusters of key-words. The main themes were identified. Alceste improves a corpus' characterization and allows a logical reading of it, thanks to the creation of categories, along with their mutual dependencies, the peculiar, meaning of each and their division in time. Moreover, Alceste allows us to perceive the contexts of the contents previously identified under Leximappe. Sampler allows us to go into the details of the terms association in graphical form and detail the specific orientations of the corpus, especially with the inscription of weak signals. Finally, this software, applied from the categories drawn from Alceste, offers for each category a meaningful graphic representation. We can argue that the different ways of measuring and presenting results are complementary since they highlight different aspects of risk assessment carried by different actors, as it is underlined in social science studies of public controversy. Moreover we can follow these actors through the categories and clusters (socioeconomic, scientific and risk assessment linked to regulation and policy) which are more and more differenciated in time. This methodology allows the study of emerging processes in the social construction of issues within controversies.

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Sivamani, E., Shen, P., Opalka, N., Beachy, R.N., Fauquet, C.M. 1996. Selection of large quantities of embryogenic calli form indica rice seeds for production of fertile transgenic plants using the biolistic method. Plant Cell Rep. 15 :322

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Baulcombe, D. C. and English, J. J. (1996): Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants. Curr. Opin. Biotechnol. 7, 173-180. Ectopic pairing of homologous DNA and post

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-free transgenic plants . In: I. Sithole-Niang (ed.): Genetic Engineering. InTech. , pp. 17 – 48 . Chu , C. C. , Wang , C. C. , Sun , C. S. , Chen , H. , Yin , K. C. and Chu , C. Y. ( 1975 ): Establishment of an efficient medium for anther

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Cereal Research Communications
Authors: Sixin Liu, Michael Pumphrey, Bikram Gill, Harold Trick, Julia Zhang, Jaroslav Dolezel, Boulos Chalhoub, and James Anderson

DNA markers near Fhb1 , a major QTL for Fusarium head blight resistance on chromosome 3BS in wheat, were used to screen the Chinese Spring chromosome 3B BAC library, and a BAC contig spanning the Fhb1 region was constructed. The genic regions of two overlapping BAC clones were sequenced. Based on new DNA markers developed from the BAC sequences, Fhb1 was narrowed down to a 261 kb region with seven putative genes. The expression of the candidate genes was examined by RT-PCR. Four out of the seven genes are expressed in wheat spikes. But, there is no clear expression difference between water-inoculated and Fusarium-inoculated wheat spikes. Five cosmid clones containing all seven candidate genes were isolated from a cosmid library of Sumai 3. The cosmid clones were used to transform FHB-susceptible cultivar Bobwhite and transgenic plants were obtained for all cosmid clones. Transgenic plants for four out the five cosmid clones have been tested for Type II resistance to FHB, and none of them are resistant. FHB evaluation of the transgenic plants of the fifth cosmid clone is in progress. A highly diagnostic, codominant marker, UMN10, was developed and used for MAS for gene Fhb1 .

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Acta Agronomica Hungarica
Authors: L. Sági, M. Rakszegi, T. Spitkó, K. Mészáros, B. Németh-Kisgyörgy, A. Soltész, F. Szira, H. Ambrus, A. Mészáros, G. Galiba, A. Vágújfalvi, B. Barnabás, and L. Marton

Research with transgenic plants in the Agricultural Research Institute of the Hungarian Academy of Sciences is primarily related to applications that are essential for the genetic improvement of cereals. The two main directions are connected to wheat and maize breeding and are focused on improving agronomic and nutritional traits. This paper highlights experiments in these areas, which are conducted in national as well as international collaborations. The transparency of this work is ensured by the dissemination of information about approved confined field tests to the public via the internet.

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Resistance in different genotypes of tomato to Meloidogyne incognita were studied using screening and molecular studies. Lycopersicon peruvianum , a wild species of tomato showed resistance reaction to Meloidogyne incognita under artificial screening infection studies. L. peruvianum , showed least penetration rate of one nematode. In susceptible wild species L. pimpinellifolium , varieties and hybrid, feeding sites were characterized by giant cells containing granular cytoplasm and many hypertrophied nuclei. In L. peruvianum , the vascular tissues were not disturbed and epidermal layer showed lignin bodies which have accumulated and inhibited the nematodes into the roots. RT-PCR analysis to detect presence of resistance (R) gene showed a 877 bp amplicon present only in L. peruvianum . Sequence analysis of the partial cDNA from L. peruvianum showed considerable homology with already available disease resistance gene against Meloidogyne incognita . These results have important consequences in terms of transformation strategies to develop root knot nematode resistant transgenic plants.

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Acta Botanica Hungarica
Authors: Á. K. Kiss, K. Szabó, M. K. Singh, and L. Gy. Szabó

Hausmann, K., Hülsmann, N. and Radek, R. (2003): Protistology, 3rd completely revised edition. - E. Schweizerbart'sche Verlagsbuchhandlung, Stuttgart, 379 pp. (With  384 figures and 22 tables); Krammer, K. and Lange-Bertalot, H. (2004): Achnanthaceae. Kritische Ergänzungen zu Achnanthes s. l., Navicula s. str., Gomphonema. Gesamtliteraturverzeichnis Teil 2/4. Ergänzter Nachdruck. - In: Ettl, et al. (eds) (2004): Süsswasserflora von Mitteleuropa. Band 2/4. Bacillariophyceae 4. Teil. Spektrum Akademischer Verlag, Heidelberg, Berlin, 468 pp.; Larkum, A. W. D., Douglas, S. E. and Raven, J. A. (eds) (2003): Photosynthesis in algae. - In: Govindjee (ed.): Advances in photosynthesis and respiration. Volume 14. Kluwer Academic Publishers, Dordrecht, Boston, London, 479 pp.; Magurran, A. E. (ed.) (2003): Measuring Biological Diversity. - Blackwell Science Ltd., Blackwell Publishing, Oxford, 260 pp. (ISBN-632-05633-9); Pena, L. (ed.) (2004): Transgenic plants - methods and protocols (Methods in molecular biology, Vol. 286). - Humana Press, Totowa, New Jersey, 437 pp. (ISBN 1-59259-827-7);

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