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Morchella conica Pers. strains of the study were isolated from fruit bodies collected in ash-mixed forests. At first, the strains were cultured on potato dextrose agar (PDA), then on modified Murashige and Skoog (MS*) solid agar media. A normal-growing strain was chosen for the trehalase induction experiments. During the trehalase induction treatment, mycelia were grown in liquid culture containing different concentrations of trehalose. After the induction period of trehalase enzymes, physiological state of the mycelium and the oxidative stress were monitored in the vegetative mycelia by measuring the change of the malondialdehyde content, superoxide dismutase enzyme activity, the fresh and dry weight. The examined Morchella conica strain utilized the trehalose properly. The rising amount of the trehalose triggered the increase of the mycelial trehalase enzyme activity. Our results clearly proved that both neutral and acidic trehalase isoenzyme activity of the Morchella conica mycelium are inducible and are playing important role in the utilization of external trehalose.

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Abstract  

As trehalose is a glucose font and also an additive in food, a new reliable method for trehalose determination is proposed. The analytical method uses an isothermal microcalorimeter, directly relates the analyte concentration with the heat variation of the enzymatic decomposition of trehalose into two glucose molecules. The enzymatic reaction is performed inside the calorimeter in the presence of trehalase enzyme immobilized on amino activated glass beads. Through the calibration curve, the trehalose quantity in some food samples (mushrooms and honey) has been determined. The calorimetric procedure was compared to a previously identified methodology based on an amperometric biosensor.

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. 46 31 1068 Keshan, B., Ray, A. K. (2001) Effect of estradiol-17beta on cell area, lumen area and trehalase activity of posterior silk gland of

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Goddijn, O. J. M., Verwoerd, T. C., Voogd, E., Krutwagen, R. W. H. H., de Graaf, P. T. H. M., Poels, J., van Dun, K., Ponstein, A. S., Damm, B., Pen, J. (1997) Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants. Plant

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