Authors:S. Naeimi, S. Kocsubé, Zsuzsanna Antal, S. Okhovvat, M. Javan-Nikkhah, C. Vágvölgyi, and L. Kredics
, P. P. (1994) Polymerase chain reaction with universalprimers (UP-PCR) and its application to plant genome analysis. In: Adams, R. P., Miller, J. S., Goldenberg, E. M., Adams, J. E. (eds) Conservation of Plant Genes II. Utilization of Ancient and
Authors:A. Csikos, A. Hodzic, E. Pasic-Juhas, A. Javor, A. Hrković-Porobija, T. Goletic, G. Gulyas, and L. Czegledi
Species identification in food has become a prominent issue in recent years as the importance of consumer protection has increased. DNA-based species identification methods were developed by researchers in the last two decades, as these are reliable, accurate, and low-cost techniques for species identification in raw and processed food products as well. In our study, universal primers were designed to conserved regions of mitochondrial 12S rRNA. Amplicons were heat-denatured and a PCR single strand conformation polymorphism (SSCP) method was developed to identify cattle, buffalo, sheep, and goat DNA. Sensitivity of this technique was tested on DNA mixtures of cattle-sheep, cattle-goat, and cattle-buffalo and the threshold limit of cattle DNA was 5%, 5%, and 3%, respectively. One hundred and five cheeses were purchased and collected from Bosnian and Hungarian farmers, retails, and supermarkets to reveal fraud, 32 percent of them (34 cheeses) were found to be mislabelled by species.
Authors:A. Almási, R. Boros, K. Salánki, and B. Barna
One of the most important diseases of pea is caused by Pea seed-borne mosaic virus (PSbMV), which has a relatively wide host range. Since there are few varieties with resistance against the virus, and spraying insecticides is not very effective, the determination of the disease and the pathogen in the seeds is very important. Inoculum prepared from pea seeds showing typical virus symptoms caused very mild symptoms on Chenopodium amaranticolor and C. quinoa, but several chlorotic/necrotic lesions on bean (Phaseolus vulgaris) cv. Scarlet, and systemic symptoms with mosaic and curling of top leaves on bean cv. Maxidor. The detection of the virus was carried out by PCR using universal primers and virus sequence analysis. According to the phylogenetic analysis the PSbMV isolate identified in Hungary belongs to the pathotype P1 and associated with the cluster 2 isolates.
Authors:N. Pandey, A. Tiwari, G. Rao, and K. Shukla
Briddon, R. W., Bull, S. E., Mansoor, S., Amin, I. and Markham, P. G. (2002): Universalprimers for the PCR-mediated amplification of DNA β; a molecule associated with some monopartite begomoviruses. Mol