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7759 7763 Provan, J., Biss, P. M., McMeel, D., Mathews, S. (2004). Universal primers for the amplification of chloroplast microsatellites in grasses ( Poaceae ). Mol. Ecol

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, P. P. (1994) Polymerase chain reaction with universal primers (UP-PCR) and its application to plant genome analysis. In: Adams, R. P., Miller, J. S., Goldenberg, E. M., Adams, J. E. (eds) Conservation of Plant Genes II. Utilization of Ancient and

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Acta Alimentaria
Authors: A. Csikos, A. Hodzic, E. Pasic-Juhas, A. Javor, A. Hrković-Porobija, T. Goletic, G. Gulyas, and L. Czegledi

Species identification in food has become a prominent issue in recent years as the importance of consumer protection has increased. DNA-based species identification methods were developed by researchers in the last two decades, as these are reliable, accurate, and low-cost techniques for species identification in raw and processed food products as well. In our study, universal primers were designed to conserved regions of mitochondrial 12S rRNA. Amplicons were heat-denatured and a PCR single strand conformation polymorphism (SSCP) method was developed to identify cattle, buffalo, sheep, and goat DNA. Sensitivity of this technique was tested on DNA mixtures of cattle-sheep, cattle-goat, and cattle-buffalo and the threshold limit of cattle DNA was 5%, 5%, and 3%, respectively. One hundred and five cheeses were purchased and collected from Bosnian and Hungarian farmers, retails, and supermarkets to reveal fraud, 32 percent of them (34 cheeses) were found to be mislabelled by species.

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One of the most important diseases of pea is caused by Pea seed-borne mosaic virus (PSbMV), which has a relatively wide host range. Since there are few varieties with resistance against the virus, and spraying insecticides is not very effective, the determination of the disease and the pathogen in the seeds is very important. Inoculum prepared from pea seeds showing typical virus symptoms caused very mild symptoms on Chenopodium amaranticolor and C. quinoa, but several chlorotic/necrotic lesions on bean (Phaseolus vulgaris) cv. Scarlet, and systemic symptoms with mosaic and curling of top leaves on bean cv. Maxidor. The detection of the virus was carried out by PCR using universal primers and virus sequence analysis. According to the phylogenetic analysis the PSbMV isolate identified in Hungary belongs to the pathotype P1 and associated with the cluster 2 isolates.

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Taberlet, P., Gielly, L., Pautou, G. & Bouvet, J. (1991): Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant molecular Biol. , 17 , 1105–1109. Bouvet J

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Briddon, R. W., Bull, S. E., Mansoor, S., Amin, I. and Markham, P. G. (2002): Universal primers for the PCR-mediated amplification of DNA β; a molecule associated with some monopartite begomoviruses. Mol

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Acta Veterinaria Hungarica
Authors: Ádám Bálint, István Kiss, Krisztián Bányai, Imre Biksi, Katalin Szentpáli-Gavallér, Tibor Magyar, István Jankovics, Mónika Rózsa, Bálint Szalai, Mária Takács, Ádám Tóth, and Ádám Dán

84 3752 3758 Hoffmann, E., Stech, J., Guan, Y., Webster, R. G. and Perez, D. R. (2001): Universal primer set for the full-length amplification of

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. Gundersen , D. E. and Lee , I. M. ( 1996 ): Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs . Phytopathol. Medit 35 , 144 – 151

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Acta Veterinaria Hungarica
Authors: Hoonsung Choi, Sang In Lee, Shanmugam Sureshkumar, Mi-Hyang Jeon, Jeom Sun Kim, Mi-Ryung Park, Kyung-Woon Kim, Ik-Soo Jeon, Sukchan Lee, and Sung June Byun

. Appl. Microbiol. Biotechnol. 99 , 2793 – 2803 . Hoffmann , E. , Stech , J. , Guan , Y. , Webster , R. and Perez , D. ( 2001 ): Universal primer set for the full

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, Batavia. Conspectus Hepaticarum Archipelagi Indici. Taberlet, P., Gielly, L., Patou, G. and Bouvet, J. (1991): Universal primers for

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