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For a vector system in E n, we establish a class of inequalities for k-dimensional space angle (defined in this paper), and give some application thereof.

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Nowadays, identification of the novel physio-biological and therapeutic functions of plant cysteine proteinase inhibitors “plant cystatins / phytocystatins” are the great of interests for molecular biologists. Whether for biochemical, structural or functional studies, their successful expression along with an easy purification method is required. To date, fusion tags are the best available tools that meet all those requirements. We report here the cloning and simple functional expression and purification of a barley putative cystatin in Escherichia coli cells. For the first time, a part of barley coding sequence containing a predicted active cystatin was amplified by polymerase chain reaction and expressed as maltose binding fusion protein in TB1 strain of E. coli cells using pMALc2X over-expression vector system without affecting the bacterial growth. The expressed product was purified by single step affinity chromatography from the soluble fraction of induced culture at a yield of about 37 mg/ liter of bacterial cell culture. The purified fused protein could efficiently inhibit papain activity in vitro without the cleavage of the fusion partner.

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-1 using a two vector system. Plant Cell Tiss. Org. Cult. , 95 , 363–371. Kumar N. Agrobacterium mediated genetic transformation of summer squash (Cucurbita pepo L. cv

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Boerkoel, C. F., Federspiel, M. J., Salter, D. W., Payne, W., Crittenden, L. B., Kung, H. J., Hughes, S. H. (1993) A new defective retroviral vector system based on the Bryan strain of Rous sarcoma virus

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adjuvant and antigen vector systems. Vaccine 11 , 1179–1184. Czerkinsky C. Cholera toxin and cholera B subunit as oralmucosal adjuvant and antigen vector systems

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manufacturer’s recommendations. Cloning of erp, hspR, lppX, mmaA4 , and ompA genes The PCR products were first ligated to pGEM-T Easy Vector System (Promega, WI, USA) according to manufacturer’s recommendations, and

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Acta Microbiologica et Immunologica Hungarica
Authors: Mónika Knáb, Tibor Szili-Kovács, Károly Márialigeti, János Móga and Andrea K. Borsodi

. Clone libraries were constructed based on blue–white selection method [ 24 ]. The purified PCR products were ligated into TA-cloning vectors (pGEM-T Vector System, Promega, USA) than transformed into competent Escherichia coli JM109 cells. To retrieve

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pGem ® -T Vector System (Promega, Madison, USA) according to manufacturer’s instructions. Insert sequences were amplified using M13 primer sets [ 42 ] followed by nested PCR reaction with the applied specific primers (27F-519R, A340F-A934R, amoA –F1

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