Authors:Ariel Imre, Ama Szmolka, Ferenc Olasz, and Béla Nagy
The aim of these studies was to assess residual virulence and early protective capacity of a negatively markered live attenuated vaccine candidate Salmonella Enteritidis mutant against a highly virulent S. Enteritidis strain using a dayold chicken model. Nonflagellated FliD negative mutants of Salmonella Enteritidis 11 (SE11) with and without the virulence plasmid proved to be sufficiently attenuated (limited invasiveness in vitro/in vivo) without reduced ability to colonise chicken gut. The early protective activity of a nonflagellated, virulence-plasmidcured (fliD–, pSEVΔ) mutant against organ invasion, caecal colonisation and faecal shedding by the highly virulent challenge strain S. Enteritidis 147 NalR proved to be effective and safe. The innate and adaptive immunity was demonstrable during the first four weeks of life, and the serological response was clearly distinguishable from the response induced by the wild parental strain. In conclusion, we provided data for the first time about a virulence-plasmid-cured, nonflagellated mutant of S. Enteritidis to serve as a basis for development of a negatively markered potential live oral vaccine against virulent S. Enteritidis in chicken.
Authors:L. Makrai, K. Kira, A. Kono, Y. Sasaki, T. Kakuda, S. Tsubaki, L. Fodor, J. Varga, and S. Takai
virulenceplasmids in the isolates from infected foals, dog and monkey. Onderstepoort J. Vet. Res. 68 ,105-110.
Prevalence of virulent Rhodococcus equi in isolates from soil collected from 2 horse farms in South Africa and
Authors:Mateja Pate, Irena Zdovc, Tina Pirš, B. Krt, and M. Ocepek
of virulent Rhodococcus equi based on restriction fragment length polymorphism of virulenceplasmids: a molecular approach for epidemiology of virulent R. equi in the world. J. Clin. Microbiol. 37 , 3417-3420.
Authors:László Makrai, László Fodor, István Hajtós, János Varga, and Béla Dénes
, T. , Tsubaki, S. , Major, A. , Fodor , L. , Varga , J. and Takai , S. ( 2005 ): Characterization of virulenceplasmids and serotyping of Rhodococcus equi isolates from submaxillary lymph nodes of pigs in Hungary . J. Clin. Microbiol. 43
Authors:Ama Szmolka, Barbara Lestár, Judit Pászti, Péter Z. Fekete, and Béla Nagy
, P. Z. , Blum- Oehler , G. , Boldogkoi , Z. and Nagy , B. ( 2005 ): Characterization of an F18+ enterotoxigenic Escherichia coli strain from post weaning diarrhoea of swine, and of its conjugative virulenceplasmid pTC . FEMS Microbiol. Lett
biological behaviour of the hvKp strains is partly mediated by genes on a large virulenceplasmid or within chromosomal islands [ 13–17 ], some of these regions including mucoviscosity-associated gene A ( magA ), regulator of mucoid phenotype ( rmp ) A and A2
Authors:Fatima Bachir Halimeh, Rayane Rafei, Seydina Diene, Mary Mikhael, Hassan Mallat, Marcel Achkar, Fouad Dabboussi, Monzer Hamze, and Jean-Marc Rolain
(enteroinvasive E. coli ) share similar genetic (analogous virulenceplasmid) and biochemical features [ 7 ]. Unlike E. coli , Shigella isolates are less active biochemically and react with limited set of antisera and shared with EIEC pathogenicity genes [ 8