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Chaudhary, H.K. 2008a. Dynamics of wheat × Imperata cylindrica — a new chromosome elimination mediated system for efficient haploid induction in wheat. In: Appels, R. et al. (eds), Proceedings of the 11th International Wheat Genetics Symposium. University of

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An investigation to standardize the protocol for in vitro application of colchicine for enhancing the doubled haploid production in wheat was done. Two tetraploid (PDW-314 and A-9-30-1); and two hexaploid (DH-40 and C-306) wheat genotypes were used as maternal parents, whereas, the pollen sources involved Zea mays (cv. Bajaura Makka) and Imperata cylindrica. During the rabi seasons of years 2013–14 and 2014–15, wheat × maize and wheat × I. cylindrica hybridization was carried out followed by treatment of their haploids produced as a result of elimination of chromosomes of maize and I. cylindrica respectively, with varied doses of colchicine for different durations The various doses of colchicine were categorized into two groups: lower doses for longer durations (0.01, 0.025, 0.05% each for 5, 7, 9, 11 hrs) and higher doses for shorter durations (0.05, 0.075, 0.10, 0.15, 0.20, 0.25% each for 5, 4, 3, 2 hrs). The response of different concentrations of colchicine applied for varied durations revealed significant differences for various doubled haploidy parameters viz., per cent survived plants, per cent doubled haploid formation and per cent doubled haploid seed formation. In hexaploid and tetraploid wheats, colchicine doses of 0.075% for 4 hrs and 0.15% for 4 hrs, respectively were established as optimum for enhanced doubled haploid production.

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.K. 2008 a. Dynamics of wheat × Imperata cylindrica –a new chromosome elimination mediated system for efficient haploid induction in wheat . In: Appels , R. Eastwood , R. , Lagudah , E. , Langridge , P. , Mackay , M. , McIntyre , L. , Sharp

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Chaudhary H.K. 2008. Dynamics of wheat Imperata cylindrica — Anew chromosome elimination mediated system for efficient haploid induction in wheat. In: Appels R., Eastwood R., Lagudah E., Langridge P., Mackay M., McIntyre L., Sharp P. (eds), Proc. 11th

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-London 82 : 29 – 33 . Chaudhary , H.K. 2008 . Dynamics of wheat × Imperata cylindrica – a new chromosome elimination mediated system for efficient haploid

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. Chaudhary , H.K. , Tayeng , T. , Kaila , V. , Rather , S.A. 2013 . Enhancing the efficiency of wide hybridization mediated chromosome engineering for high precision crop improvement with special reference to wheat × Imperata cylindrica

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