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Brunetto, M. R., Giarin, M. M., Oliveri, F. et al.: Wild-type and e antigen-minus hepatitis B viruses and course of chronic hepatitis. Proc. Natl. Acad. Sci. USA, 1991, 88 , 4186. Oliveri F

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Acta Biologica Hungarica
Authors: Zoltán Gazdag, Timea Stromájer-Rácz, Joseph Belagyi, Richard Y. Zhao, Robert T. Elder, Eszter Virág and Miklós Pesti

dehydrogenase HIV-1 Vpr human immunodeficiency virus type-1 viral protein R NL4-3Vpr wild-type Vpr

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effect of an incompatible wild type of, a hypersensitive response (HR) mutant of and saprophytic Pseudomonas bacteria on membrane permeability, PSII efficiency and metabolic activity of oilseed rape cotyledons with and without brassinosteroid pre

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Abstract  

It is claimed, though not without dispute, that genetically engineered mammalian cells grow more slowly than their progenitor cells because the recombinant gene system causes a metabolic burden. This was found to be the case for CHO cells transfected with expression vectors forcytochrome b5. The slower growth was associated with lower metabolic activity measured by heat flux and mitochondrial activity (rhodamine 123 fluorescence). The calorimetric-respirometric ratio was similar for all cell types, implying that the greater fluxes of glucose and glutamine in the recombinant cells was channelled to biosynthesis. This demand probably restricted the supply of pyruvate to the mitochondria in these cells.

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Abstract  

In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O4) was characterized by FT-IR. The particle size was shown to be ≈5–10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling efficiency (over 96%, RTLC method) and they also showed an excellent stability at room temperature for at least 2 days and were evaluated for their biodistribution in normal rats up to 24 h compared with free Ga3+ cation and [67Ga]-SPION biodistribution. The biodistribution of the tracer among 3 other folate tracers were compared, showing lower liver uptake and higher blood circulation after 24 h leading to better bioavailability. The bone:muscle, kidney:muscle, lung:muscle, stomach:muscle ratios were 9.3, 9.32, 7.6 and 5.83 respectively. The developed folate-containing nano-system can be an interesting folate receptor tracer, capable of better cell membrane permeability while possessing paramagnetic properties for thermotherapy.

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, C., von Wettstein, D., Schäfer, W., Kogel, K.H., Felk, A., Maier F.J. 2005. Infection patterns in barley and wheat spikes inoculated with wild-type and trichodiene synthase disrupted Fusarium graminearum . PNAS 46 :16892

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Neurospora crassa : studies with the wild-type strain and mutants wc-1, wc-2 and acon-2 . J. Photoch. Photobiol. B: Biol. 37, 203–211. Yanofsky C. Light stimulation of conidiation in

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. (1998) Light and temperature cooperate to regulate the circadian locomotor rhythm of wild type and period mutants of Drosophila melanogaster . J. Insect Physiol. 44 , 587–596. Matsumoto A

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utilization in Drosophila . Analysis of (a) gynandromorphs; (b) wild-type females that were decapitated, or their ventral nerve cord (VNC) was cut through; and (c) females from which the germline cells were genetically deleted clearly showed that MP expelling

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51 1267 1277 Proctor, R. H., Hohn, T. M. and McCormick S. P. (1997): Restoration of wild-type virulence to Tri5 disruption mutants of

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