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References Alam , M. , Gomes , I. , Mohuiddin , G. and Hoq , M. M. ( 1994 ): Production and characterization of thermostable xylanase by Thermomyces lanuginosus

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Xylanase (EC3.2.1.8) is an industrially important enzyme that hydrolyzes xylan by breaking the hemicelluloses of the plant cell wall and produces xylooligosaccharides, xylobiose, and xylose ( B eg et al., 2001 ; P aes et al., 2012 ). This

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Anand, L., Krishnamurthy, S., Vithayathil, P. J. (1990) Purification and properties of xylanase from the thermophilic fungus, Humicola lanuginosa Griffon and Maublanc Bunce. Arch. Biochem. Biophys. 276 , 546

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Bata, Á., Kutasi, J., Jurkovich, V., Fébel, H. and Rafai, P. (2002): Activity and stability of a fungal 1,4-beta-endo-xylanase preparation in the rumen of sheep. J. Anim. Feed Sci. 11 , 627

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Progress in Agricultural Engineering Sciences
Authors: Balázs Lemmer, Szabolcs Kertész, Gábor Keszthelyi-Szabó, Kerime Özel, and Cecilia Hodúr

Membrane separation processes are currently proven technologies in many areas. The main limitation of these processes is the accumulation of matter at the membrane surface which leads to two phenomena: concentration polarization and membrane fouling. According to the publications of numerous authors permeate flux could be increased by sonication. Our work focuses on separation of real broth by sonicated ultrafiltration. The broth was originated from hydrolysis of grounded corn-cob by xylanase enzyme. The filtration was carried out in a laboratory batch stirred cell with a sonication rod sonicator. In our work the effect of the stirring, the intensity of sonication and the membrane-transducer distance was studied on the efficiency of the ultrafiltration and on the quality of separated enzymes. Results reveal that xylanase enzyme can be effectively separated from real fermentation broth by ultrafiltration and enzymes keep their activity after the process. Enzyme activity tests show that low energy sonication is not harmful to the enzyme.

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1987 Ghangas, G. S., Hu, Y. J., Wilson, D. B. (1989) Cloning of a Thermomonospora fusca xylanase gene and its expression in Escherichia coli and

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Akyol, I., Comlekcioglu, U., Kar, B., Ekinci, M. S., Ozkose, E. (2009) Cloning of a xylanase gene xyn2A from rumen fungus Neocallimastix sp. GMLF2 in Escherichia coli and its partial characterization. Biologia 64 , 664

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. & Tan, S. (2005): Improvement of the breadmaking quality of wheat flour by the hyperthermophilic xylanase B from Thermotoga maritima . Fd Res. int. , 38 , 37–43. Tan S

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. & P outanen , K. ( 1992 ): Interlaboratory testing of methods for assay of xylanase activity . J. Biotechnol ., 23 , 257 – 270 . B aillet , E. , D owney , G. & T uohy , M. ( 2003

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Purkarthofer, H. and Steiner, W. 1995: Induction of endo-beta-xylanase in the fungus Thermomyces lanuginosuspp. Enzyme Microb. Technol. 17 , 114-118. Induction of endo-beta-xylanase in the fungus Thermomyces lanuginosus

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