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Biology is a study of living objects and their life processes. It examines all aspects of living organisms such as their occurrence, classification, internal and external structure, nutrition, reproduction, inheritance, etc. The term “biology” is commonly replaced by the terms “life sciences” and “biological sciences.” There are dozens of branches of biology. Some of the major ones include:
Biology and Life Sciences
Abstract
Allostatic load (AL) is a comprehensive physiologic measure of the body's chronic stress response and is associated with physical and mental health risks. The function of DASH diet (Dietary Approaches to Stop Hypertension) model in the development of AL is unclear. The relationship between the DASH score and AL was examined in this study. 1,565 US adults from NHANES database 2017–2020 were selected for the study, and DASH dietary pattern was assessed using DASH score, and ≥4.5 indicated compliance with DASH diet. AL was calculated using 11 biomarkers, and a score >3 indicated high levels. The relationship between DASH score and AL was analysed using logistic regression. In this study, a high AL prevalence of 35.4% (n = 555) was found. In the unadjusted model, a high DASH score was associated with a low level of AL [OR = 0.458, 95% CI (0.306, 0.687), P < 0.001], and this relationship persisted in the adjusted model [OR = 0.473, 95% CI (0.310, 0.720), P < 0.001]. DASH score are negatively associated with AL, and low DASH score increase the risk of high AL, which can adversely affect physical and mental health.
Abstract
Antibiotic resistance is a major problem in the healthcare industry, and it presents difficulties in managing bacterial diseases worldwide. The need to find alternative antibiotic-containing methods is thus a major area for the scientific community to work on. Bacteriophage therapy is an interesting alternative that has been used in scientific research for a long time to tackle antibiotic-resistant bacteria. The purpose of this review was to compile the latest data on bacteriophages, which are progressively being used as alternatives to antibiotics, and to identify the mechanisms associated with phage therapy. The results section delves into the growing challenges posed by antibiotics and explores the potential of bacteriophages as therapeutic alternatives. This study discusses how phages can decrease antibiotic resistance, highlighting their role in modulating microbiomes and addressing various complications. This study explored the intriguing question of whether bacteriophages can combat nonbacterial diseases and examined their indirect use in pest control. In addition, this study explores the application of the CRISPR-Cas system in combating antibiotic resistance and specifically addresses phage therapy for secondary bacterial infections in COVID-19. We will further discuss whether bacteriophages are a noteworthy alternative to antibiotics by considering the evolutionary trade-offs between phages and antibiotic resistance. This section concludes by outlining future perspectives and acknowledging limitations, particularly in the context of phage and CRISPR-Cas9-mediated phage therapy. The methodology adopted for this study is a comprehensive research strategy using the Google Scholar and PubMed databases, among others. In conclusion, phage therapy is a promising strategy for tackling antibiotic-resistant bacteria, contributing to improved food production and mitigating secondary health effects. However, effective regulation requires careful selection of phages in conjunction with antibiotics to ensure judicious control of the coevolutionary dynamics between phages and antibiotics.
Abstract
This study investigated the effects of steaming and blanching pre-treatments on the physicochemical properties, bioactive compound content, and antioxidant activity of black garlic during fermentation. Moisture content and pH decreased over fermentation time, while titratable acidity increased. Pre-treatments enhanced the total phenolic, flavonoid, and flavonol contents, as well as antioxidant activity (DPPH, ABTS+, FRAP), compared to control samples, with steaming pre-treatment being more effective than boiling. 5-HMF and S-allyl cysteine (SAC) levels were optimised with pre-treatments, promoting the formation of bioactive compounds. These methods offer an efficient, cost-effective way to enhance black garlic production, shorten processing times, and improve its nutritional and economic value.
Abstract
Children have increased their screen time in recent years. The aim of this study was to describe the use of smartphone, tablet, and television (TV) during breakfast, lunch, and dinner in a sample of Spanish children from three months up to five years. A cross-sectional study was performed using a convenience sample, with 410 parents of Spanish children aged three months to five years. Data were collected from March 2021 to March 2022 using an ad hoc, self-administered, online questionnaire. Prevalence of use of smartphone, tablet, and TV during meals were calculated overall and according to characteristics of the child (i.e., sex and age of the child, having siblings, having older siblings) and characteristics of the respondent (i.e., relationship with the child, age, educational level). Chi-squared tests of independence were carried out to explore the association between smartphone, tablet, and TV usage and independent variables. The prevalence of use of any screen device was 18.3% during breakfast, 18.5% during lunch, and 19.0% during dinner. The percentage of smartphone, tablet, and TV use was higher in children aged two to five years compared to those from three months to two years, with statistically significant differences in the percentage of TV use during lunch and dinner between the two age groups and in the percentage of tablet use during dinner. In addition, the probability of using each of the devices at each of the meals was lower for children of parents with university educational level than for children of parents with non-university educational level. Overall, the use of screen devices during the mealtimes in this sample of children in Spain is high. Screen time should be avoided during mealtimes to encourage healthier behaviours, such as family communication and learning proper eating habits.
Abstract
B-cells serve as a niche for Salmonella to establish a chronic infection, enabling bacteria to evade immune responses. One mechanism Salmonella uses to survive inside B-cells involves inhibiting the NLRC4 inflammasome activation, thereby preventing pyroptotic cell death. This study investigates whether Salmonella-infected B-cells can mount bactericidal responses to control intracellular bacteria. Our results show that Salmonella-infected B-cells can produce and release TNFα, IL-6, and IL-10, but not RANTES. Furthermore, priming B-cells with TNFα, IL-1β, or IFNγ enhances their bactericidal activity by promoting the production of reactive oxygen and nitrogen production species, reducing intracellular load. These results suggest that B-cells can clear Salmonella infection within a pro-inflammatory environment. However, the concurrent production of IL-10 may counteract the effects of pro-inflammatory cytokines, potentially modulating the immune response in the microenvironment.
Abstract
Multi-drug resistant bacterial infections are of global concern, leading to staggering health care costs and loss of lives. Hence, novel therapeutic options are highly required. Garcinia mangostana, a plant known as mangosteen (also termed “queen of the fruits”), is said to possess a multitude of favorable features like anti-microbial capacity. Accordingly, we compiled a literature review addressing the potential of the mangosteen and its compounds for the treatment of bacterial infections. The included 23 publications consistently reported the inhibition or elimination of bacteria following the administration of mangosteen extracts and compounds such as the xanthone α-mangostin, both in vitro and in vivo. Even pathogens like methicillin-resistant Staphylococcus aureus as well as vancomycin-resistant Enterococcus species were tackled. While the effect of mangosteen extracts and compounds appeared to be dose-dependent, they exhibited also anti-biofilm activity and strong stability under varying conditions, suggesting suitability for a versatile approach to combat infectious diseases. Moreover, the combination of α-mangostin with other phytotherapeutic agents and especially antibiotics revealed enhanced anti-bacterial results, at low or no toxicity. In light of this review, we conclude that mangosteen extracts and compounds are promising candidates for the anti-bacterial therapy of human infections, warranting further consideration in clinical trials.
Abstract
Two Muscat grapes, Zaoheibao and Xiahei, with strong aroma were selected, co-fermented with Cabernet Sauvignon grapes (CFZ and CFX) in different proportions, and dried (CZD and and CXD). The physicochemical characteristics, colour, and volatile compounds in wine samples were measured. Compared with Cabernet Sauvignon wine, drying reduced the aroma compounds and colour but increased phenols content, improving overall quality based on sensory evaluation, especially for Zaoheibao (CZD, 3:1), which obtained the highest sensory score accompanied by increased rose oxide and phenols contents. Regarding Xiahei wine, different treatments had different effects. CFX wines co-fermented in a ratio of 1:3 and 3:1 have prominent aroma compounds. After drying, CXD wines co-fermented in a ratio of 3:1 have a more prominent taste. This research puts forward new innovation points and makes an in-depth analysis and research on specific problems.
Abstract
This study examined the effect of grapefruit seed extract (GSE) (0%–3%) on the stability of total phenolic content (TPC), anthocyanins, and colour in aronia juice under heat treatments (60 °C, 80 °C for up to 120 min). TPC and anthocyanins were measured using spectrophotometry and HPLC. The highest TPC (8545.02 ± 355.55 GAE mg L−1) was measured in aronia juice with 1% GSE after 60 min at 80 °C. The highest anthocyanin retention (3178.75 mg L−1) was detected in the sample with 1% GSE after 5 min at 60 °C. Cyanidin-3-galactoside was the most abundant anthocyanin, followed by cyanidin-3-xyloside and cyanidin-3-arabinoside. Cyanidin-3-arabinoside showed the lowest heat stability, while cyanidin-3-galactoside was relatively more stable. This study, the first to evaluate anthocyanin half-life values in chokeberry juice, suggests that 1% GSE enhances colour stability during heat treatment, supporting its use as a natural food additive.
Abstract
Legumes are not only known to hold many nutritional values, including minerals such as iron, but also to contain antinutrients that can inhibit mineral absorption. Fermentation and germination are deemed simple, accessible, and sustainable ways to improve the nutritional composition of legumes. This study aimed to investigate the effect of spontaneous fermentation and germination on the antinutrients and iron levels of several legumes, consisting of soybean, kidney bean, and mung bean. Each legume type underwent spontaneous fermentation through soaking for 72 h and germination for 72 h, followed by milling to obtain their flours. The results indicated that spontaneous fermentation and germination could effectively reduce all legumes' tannin and phytic acid contents. However, while spontaneous fermentation increased iron content in all legume types, germination decreased iron content in soybean and kidney bean flours. This study has revealed that spontaneous fermentation served as the most effective method to reduce antinutrients and enhance iron levels in legumes. Therefore, this study is expected to support the fulfilment of the body's iron needs through legume's simple processing methods, such as spontaneous fermentation.
Abstract
This study investigates the flavour profile of volatile compounds in venison-based prepared food subjected to various reheating methods. Gas chromatography-ion mobility spectrometry (GC-IMS) and electronic nose technology were employed to detect and analyse the characteristic flavour of three types of venison prefabricated food samples, which underwent different reheating treatments. During the research, 41 distinct volatile compounds were identified from the venison prefabricated food samples. Following normalisation of ion peaks and three statistical analysis techniques, 21 types of volatile organic compounds (VOCs) were detected effectively from dishes reheated by air fryer. According to data analysed by the odour description database, the venison-containing dishes exhibited superior retention of wine and onion aromas upon reheating. The results of electronic nose detection indicated the presence of benzaldehyde, alcohols, aromatics, hydrocarbons, nitrogen oxides, and aliphatic aromatic substances in braised venison prefabricated vegetables; especially benzaldehyde and alcohols displayed similar aroma characteristics as detected by GC-IMS. Orthogonal partial least squares discriminant analysis (OPLS-DA) identified key variables contributing significantly to the volatile flavour profiles in venison prepared vegetables across different reheating methods: The main odourants were ethanol, undecane D, 1-propanol, ethyl acetate (acetic acid ethyl ester), 3-methylbutan-1-ol, n-pentanal, 6-methyl-5-hepten-2-one, 1-butanol, butanal, and other 30 compounds (VIP > 1).
Abstract
Antibiotics are employed worldwide for both prevention and therapeutic purposes against infectious diseases on dairy farms. Recurrent infections frequently arise from biofilm-forming bacteria. This research aimed to evaluate the efficacy of oak honey, honeycomb capping, and propolis against certain biofilm-associated and antibiotic-resistant bacteria. The total phenolic content (TPC) values for propolis, honeycomb capping, and oak honey samples were 298, 128, and 72 mg of gallic acid equivalent (GAE)/100 g, respectively. Oak honey showed the lowest minimum inhibitory concentration (MIC) value (<0.08%) against the strong biofilm of non-bla CTX-M Serratia fonticola-1. Propolis had the lowest MIC value (<0.03%) against the weak biofilm of bla CTX-M positive Citrobacter braakii, and the moderate biofilm of non-bla CTX-M S. fonticola-2. The MIC values of all bee products showed a significant decrease upon interaction with 1% boric acid for all strains. Moreover, propolis has shown the ability to reduce 96% of the biofilm biomass of Citrobacter braaki after 24 h. The results show that bee products, used for medicinal purposes since ancient times, have emerged as a viable alternative against environmental pathogens.
Abstract
An alternative to roller mills, a French-designed stone mill, was evaluated and compared with traditional laboratory roller mills through grinding performance. Developed by the Astrié brothers in the 1950s, the stone mill's slow-rotating granite stones purportedly preserve the full nutritional value of the grain. This study, conducted in collaboration with the Csoroszlya farm, compared flour from the same wheat batch ground by both an Astrié stone mill and a laboratory roller mill. Evaluations included the Pekar test for bran content, sieve analysis, measuring wet gluten content, and gluten index to assess protein quality. Rheological properties of the doughs were analyzed using Farinograph and Mixolab equipment, which indirectly also measured amylase enzyme activity. Results indicated that the stone mill produced flour with finer grain size, higher protein content, and higher enzyme presence, although challenges remain in achieving optimal gluten index and dough stability.
Abstract
Sorghum is rich in phenolic compounds, such as phenolic acids, flavonoids, and anthocyanins. Among them, 3-deoxyanthocyanin (3-DXA) is a rare subclass of anthocyanins, which has not only antioxidant, anti-inflammatory, and anti-cancer functions, but also good thermal stability, colouring effect, as well as unique properties of acid and alkali resistance. In this study, we extracted 3-DXA from sorghum grains and analysed its composition. Component analysis indicated that the purified 3-DXA mainly contained apigenin and luteolin, with the contents of 1.46 μg g−1 and 1.38 μg g−1, respectively. The antioxidant activity of 3-DXA in vitro was between vitamin C (Vc) and rutin based on 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical scavenging rate and Fe3+ reducing power. Different concentrations of purified 3-DXA were applied to pork meat stored at 4 °C. After 11 days of storage, physiological and biochemical indices related to antioxidant and meat quality were determined. The results showed that 0.02% 3-DXA had the best antioxidant effects on pork meat within 11 days of storage at 4 °C based on a* value, pH value, purge loss, TBARS value, and total number of bacteria. The results in this study are helpful for the application of 3-deoxyanthocyanin to maintain the quality of pork meat and prolong the storage time at 4 °C.
Abstract
Epigenetic modifications are critical for the regulation of CD4+ T cell differentiation and function. Previously, we identified Acyl-CoA Synthetase Bubble Gum 1 (Acsbg1), a gene involved in fatty acid metabolism, as part of an epigenetic signature that was selectively demethylated in ex vivo isolated T helper 17 (TH17) cells. However, its functional relevance for CD4+ T cells remains incompletely understood. Here, we used in vitro differentiation assays and the adoptive transfer colitis model to investigate the role of Acsbg1 in the differentiation and function of TH1, TH17, and regulatory T (Treg) cells. In vitro, Acsbg1 was expressed in both TH17 and in vitro-induced Treg (iTreg) cells, whereas TH1 cells lacked Acsbg1 expression. Accordingly, Acsbg1 deficiency resulted in impaired TH17 and iTreg differentiation, whereas TH1 differentiation was unaffected. In vivo, upon adoptive transfer of Acsbg1⁻/⁻ Tnaïve cells, immunodeficient recipient mice exhibited an exacerbated colitis, characterized by an altered balance of TH17 and Treg cells, indicating that Acsbg1 expression is essential for optimal TH17 and Treg cell differentiation and function. Our findings highlight the importance of fatty acid (FA) metabolism in maintaining immune homeostasis by regulating T cell differentiation and provide novel insights into the metabolic targeting of inflammatory diseases.
Abstract
Aronia melanocarpa, a main constituent of black chokeberry, provides a rich source of bioactive molecules including polyphenols, flavonoids, and anthocyanins and has been used for long in traditional medicine due to its various health-promoting and disease-alleviating properties. The aim of our literature survey was to provide an actual update of evidence regarding the antibacterial activities exerted by Aronia melanocarpa and its potential application for the treatment of human bacterial pathogenic including food-borne infections. Our survey revealed that distinct ingredients in Aronia melanocarpa do not only inhibit growth of Gram-positive and to a lesser extent of Gram-negative bacteria, but also biofilm formation that is even more pronounced upon combined application. Furthermore, the anti-microbial effects against food-spoiling bacteria underscores the application of defined Aronia-derived molecules in food preservation decreasing the risk for transmission of food-borne pathogens and thereby, improving food safety. Notably, in vivo studies revealed that prophylactic Aronia juice application alleviated murine Listeria monocytogenes-induced enteritis, dampened growth of streptococci involved in dental caries development, and decreased the incidence of urinary tract infections in nursing home residents. In conclusion, Aronia-derived bioactive molecules exhibit promising anti-bacterial and disease-alleviating effects that should be further elucidated in clinical studies.
Abstract
Conventional laboratory mice are protected from oral Campylobacter jejuni infection due to colonization resistance (CR) mediated by their host-specific gut microbiota. Here, we used differential effects of distinct antibiotics on gut microbiota composition to identify microbial groups associated with CR against C. jejuni. Therefore, specific pathogen-free (SPF) mice were subjected to ampicillin plus sulbactam (A/S), ciprofloxacin (CIP), or vancomycin (VAN) via the drinking water for 28 days or left untreated before peroral C. jejuni challenge. Cultural analyses revealed that CR displayed by untreated mice was abrogated by A/S treatment, but only reduced in mice treated with CIP or VAN. Notably, differential analysis of antibiotic-induced microbiota changes and C. jejuni colonization dynamics identified lactobacilli and Clostridium leptum as key microbial groups that were associated with CR. Notably, the complete eradication of intestinal bacteria in A/S treated mice supported high intestinal C. jejuni colonization levels which triggered apoptosis and inflammatory responses accompanied by enhanced expression of matrix-degrading gelatinases in the colon. In conclusion, A/S treated mice represent a valuable infection model for the study of campylobacteriosis and the treatment of mice with specific antibiotics support the investigation of molecular mechanisms involved in CR against enteropathogens.
Abstract
The main problem in incorporating probiotics to hurdle matrix of foodstuffs is mitigation of viable count in the gastrointestinal tract. This study investigated the effects of various dairy matrices (sterilised milk, doogh, UF cheese, and yoghurt) on the viability of different probiotics under simulated gastrointestinal conditions. The results showed that the highest and the lowest viability in milk were related to Lacticaseibacillus paracasei and Bifidobacterium lactis, respectively. In doogh samples, no significant difference was observed. In cheese, the highest viability was observed in Lactobacillus acidophilus followed by Bifidobacterium longum, and L. paracasei. The viability of probiotics in yoghurt was less than in cheese and control treatment. These results indicated that milk and doogh are efficient delivery matrices for carrying probiotic cells.
Abstract
Natural preservatives are a suitable alternative to chemical preservatives in the food industry. To overcome its hydrophobic nature, insolubility in water, and degradation of free essential oils, liposomal encapsulation can be applied. In this study, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of free as well as liposomal rosemary essential oils (REO) were measured using disk diffusion and serial dilution method for Escherichia (E.) coli, Staphylococcus (S.) aureus, Salmonella (S.) enterica, Lactiplantibacillus (L.) plantarum, Aspergillus (A.) niger, and Saccharomyces (S.) cerevisiae. The antimicrobial properties of free and encapsulated essential oils were compared with sodium benzoate during thirty days of storage. Also, the release pattern of REO from liposomes was studied using dialysis membranes during the 30 days of storage. The results showed that the MIC of free and encapsulated REO is in the range of 3.5–5% and 5–6.5%, respectively. Gram+ Staphylococcus aureus was identified as the most sensitive, while Escherichia coli was the most resistant among the tested microorganisms to the essential oils. During refrigerated storage, free REO and sodium benzoate did not show any significant difference in antimicrobial properties (P > 0.05), while encapsulated REO significantly reduced the number of microorganisms in stored salad dressing (P ≤ 0.05).
Abstract
A successful spray-drying encapsulation is supported by the use of suitable matrix material. This research aimed to study the properties of maltodextrin-alginate matrix solution and its encapsulated iron powder. The study was conducted using the variation of iron concentration (8–20 mg g−1 matrix) and ratio of maltodextrin-alginate (10:0; 10:0.5; 10:1; 10:1.5; 10:2; 0:2). The results showed that a higher iron concentration increased the viscosity of the matrix solution, which led to the formation of higher moisture content particles, but reduced the encapsulation efficiency. Meanwhile, an increased ratio of maltodextrin-alginate allowed an increase in the viscosity of the matrix solution, the encapsulation efficiency, and the moisture content of the particles. However, iron loading capacity improved by increasing iron and alginate concentration. All samples appeared to be amorphous materials with a faster iron release at a pH 6.8 than that at a pH 1.2. In addition, incorporation of alginate improved the particle size, thermal stability, and antioxidant activity of the encapsulated iron powder. In brief, the improvement of the encapsulation efficiency, iron release, and thermal stability of spray-dried iron powder are highlighted in this study, which are important in food fortification and processing.
Abstract
This study successfully microencapsulated bitter gourd juice (EBG) and turmeric powder (ET) using chickpea protein isolate via freeze-drying, achieving encapsulation efficiencies of 85.3% and 89.5%, respectively. Particle size analysis revealed sizes of 3.69 µm for EBG and 4.97 µm for ET. FTIR confirmed the successful entrapment of core materials within the protein matrix. Although unencapsulated samples exhibited higher activity levels, the encapsulated forms also demonstrated significant antioxidant, antidiabetic, and anti-lipidemic properties, with effective protection and controlled release of active components in the gastrointestinal environment. This highlights their potential as functional ingredients for managing type 2 diabetes and hyperlipidaemia in food products.
Abstract
Basil (sweet basil, Ocimum basilicum L.) is an aromatic plant known for its culinary and traditional medicinal uses.
The content of estragole (methyl chavicol), a compound associated with a potential risk to human health, was determined in the essential oils of 12 samples of basil herb by gas chromatography with flame ionisation and mass spectrometry detection (GC-FID/MS) using an external standard method. The essential oils contained estragole at various levels, from 1.85 to 561.01 mg mL−1. Regarding the chemical profile, among the eleven essential oils, the compound with the highest relative proportion was linalool (29.1–70.3%), while estragole dominated in one essential oil with a relative proportion of 45.2%. All essential oils tested correspond to the European chemotype, which is characterised by a high content of linalool or a combination of linalool and estragole.
Given the recommendation for limiting human exposure to estragole, the safety of some essential oils may be of concern due to their high levels of estragole content.
The results indicate the importance of the chemical analysis of basil herb samples and the selection of chemotypes with low estragole content.
Abstract
Quaternary ammonium compounds (QACs) are the most significant disinfectants utilised to control the contamination of Staphylococcus aureus in food establishments. S. aureus is a significant pathogen that carries genes responsible for resistance to QACs, which pose a risk to public health and food safety. The objective of the study was to investigate the prevalence of QAC genes (qacA/B, qacC, qacG, qacH, qacJ, and smr) and benzalkonium minimal inhibitory concentration (MIC) values in S. aureus strains isolated from food products and food production facilities (n = 200). The analysis results indicated that the qacC gene was the most frequently detected, with a prevalence of 12%. The qacA/B, qacG, qacH, qacJ, and smr genes were identified at frequencies of 2%, 3%, 1%, 4.5%, and 5%, respectively. The highest MIC level was identified in the surface sample, which carried the qacG gene, at a concentration of 6.25 μg mL−1. The study's results highlight the potential risks associated with disinfectant resistance in food establishments. To prevent the transfer of disinfectant resistance genes, which have become a global risk, it is imperative that the rules of disinfectant usage are observed rigorously and that scientific research in this field is diversified.
Abstract
Bifidobacteria can maintain the ecological balance of intestinal microorganisms and are closely linked to the onset and progression of acute enteritis. Present research aimed to investigate the alleviation of DSS-induced acute enteritis by bifidobacteria. In vivo colonoscopy was performed in mice to observe the colorectal mucosa and the pathological damage of colon tissue and the colonic expression of tight junction proteins (Occludin, Claudin-1, ZO-1) and inflammatory factors (TNF-α, IL-1b, IL-6). The colon mucosa tissue samples were collected for bacterial 16S DNA sequencing and transcriptome sequencing. The intervention of bifidobacteria could effectively alleviate the trend of weight loss and colonic trauma in mice with DSS-induced acute enteritis. The bifidobacteria effectively restored expression of tight junction proteins (Occludin, Claudin-1, and ZO-1) and decreased expression of pro-inflammatory factors (TNF-α, IL-1b, IL-6). Bifidobacterium longum proved to be the most effective (P < 0.05). The altered composition of gut microbiota was reflected in the increase of the relative abundances of Dubosiella spp. in the B. longum treated group. The results suggested that bifidobacteria could repair intestinal barrier function, relieve the colon inflammation, and improve intestinal microbiota disorder; and B. longum demonstrated the best efficacy in mice with DSS-induced acute enteritis.
Soil organic carbon (SOC) levels directly affect the production and health of crops. Making use of a database of the usefulness of using the 350–2,500 nm Near Infra Red (NIR) spectroscopy data range on 200 soil samples from the Indian state of Uttar Pradesh was evaluated in this study. The more sophisticated Artificial Neural Network, to choose the spectral components that were used to forecast SOC, Random Forest (RF) and Ensemble Lasso-Ridge Regression (ELRR) were utilized. In the preprocessing, the inversion derivative, logarithmic(log) derivative, and logarithmic base to 10(log10x) derivatives were employed to duplicate the spectrum wavelength. The main characteristic of spectrum wavelength for SOC were found to be within the range of 350 and 450 nm, per the results. The best accurate estimation of SOC content was obtained by combining the suggested DSANN or Dropout Sequential ANN technique with the Log10x pre-processed data. The R-squared (R2), RMSE, and RPIQ (Ratio of Performance in Inter Quartile Distance) values for the testing dataset were 0.83, 0.08, and 4.32, respectively.
Abstract
Four new Rotundabaloghia (Circobaloghia) species are described from South America and South-East Asia based on the collection of the Natural History Museum, London, UK. Rotundabaloghia (Circobaloghia) salebrosa sp. nov. was collected in Malaysia, Rotundabaloghia (Circobaloghia) bakerae sp. nov. was found in Sarawak (Malaysia), Rotundabaloghia (Circobaloghia) microseta sp. nov. is described from Guyana and Rotundabaloghia (Circobaloghia) peritremata sp. nov. was reported from Borneo (Indonesia).
Abstract
Interferon-gamma (IFN-γ) autoantibody syndrome is an emerging clinical entity that has been associated with disseminated non-tuberculous mycobacterial infection (dNTM) particularly in healthy young people, a population not previously thought to be at particular risk. A 29-year-old South-East Asian man presented with several weeks of fever, cough, lymphadenopathy, and constitutional symptoms while working on an international cargo ship, deteriorating rapidly with a sepsis-like syndrome. Eventually lymph node and sputum cultures revealed a diagnosis of dNTM infection with growth of both Mycobacterium persicum and Mycobacterium abscessus. He was commenced on rituximab as well as combination anti-mycobacterial therapy with good clinical response. This case highlights some of the difficulties faced by patients with dNTM in the context of IFN-γ autoantibodies, particularly delayed diagnosis, and lack of evidence base surrounding optimal management. Further research into long-term outcomes and treatment is required as well as increased awareness among clinicians.
Abstract
Sugar beet (Beta vulgaris) is a strategic agricultural product in Iran, meanwhile several pests cause damage to the crop and reduce its yield. Sowing dates and planting methods might have influence on the pest population and damages. Thus, current research was carried out for studying the planting methods and date of sugar beet sowing under field conditions. The treatments were 1) sowing direct seed (SDG1 = on time-planted in April 24th, SDG2 = delay time-planted sugar beets in June 12th), 2) planting seedling (SDG3 = June 11th).
The observations for record of data were carried out at weekly intervals from germination stage or establishment of seedling up to maturity in August 27th. Results revealed that the mean injuries of pest insects especially Agrotis segetum Schiff.; Chaetocnema tibialis Illiger; Conorhynchus brevirostris Gyllenhal; Spodoptera exigua Hübner and Scorbipalpa ocellatella Boyd. were significantly different in various sowing date and methods. The incidence of these pests was relatively low during the first season than the second one. The cultivation of transplanting seedling could significantly reduce the density of the pest insects and decrease crop injury rather than others. Overall, due to the annual increasing of the temperature as global warming and thus increasing insect pests and use of pesticides; transplanting seedling could use as a non-chemical method in management of sugar beet pest insects.
Abstract
The present work evaluates the effect of the incorporation of 6% carnauba wax (CW) and 7% monoglyceride (MG) oleogels for production of a healthy lipid mixture (linseed and sunflower oils) oleogels as a fat replacer at 30, 50, and 70% in hamburger formulation. Hamburgers' fatty acid composition, cooking loss, fat absorption, and texture and oxidative stabilities were evaluated. Incorporation of oleogels in hamburger formulation resulted in a significant decrease (P < 0.05) in amounts of saturated fatty acids (SFAs), as well as a decrease in the ratio of omega-6 to omega-3 fatty acids and atherogenic indices, and an increase in linoleic and linolenic acids (PUFAs) content. Substitution of animal fat with CW and MG based oleogels improved the manufactured hamburgers' quality by reducing cooking loss and fat absorption. In addition, the use of oleogel improved the oxidative stability during the storage of burgers. The lower hardness of oleogels compared to animal fats, as well as their nutritional and technological properties, make them a desirable candidate for animal fat substitution.
Abstract
Ophelimus maskelli (Ashmead, 1900) (Hymenoptera: Eulophidae) is an invasive species that has spread to Eucalyptus in the recent years in several countries, particularly in the Mediterranean and Africa, causing significant damage to Eucalyptus plantations and nurseries. The present work consists of, firstly, a study focussing on the incidence of infestation and the population dynamics of Ophelimus maskelli on Eucalyptus camalduliensis (Myrtaceae) and secondly, a study evaluating the parasitism of this pest by Closterocerus chamaeleon (Girault, 1922) (Hymenoptera) during two years (2019 and 2020) in two different forests in the region of Oued Smar (Algiers). The study revealed that the incidence of infestations is very high and Closterocerus chamaeleon, the parasitoid of Ophelimus maskelli used in several countries successfully, is present with a considerable rate of parasitism in the two sites; these results are discussed. This invasive species, which has spread in the recent years, requires further investigation in order to develop effective pest management methods that could possibly limit its spread.
Abstract
Pathogenicity test was conducted of three beetroot (Beta vulgaris) cultivars using five different inocula of Meloidogyne incognita and Pseudomonas syringae pv. aptata. Plant growth parameters, chlorophyll and carotenoid contents were reduced significantly over control when 2000 or more second stage juveniles (J2) of M. incognita or 10.0 mL or more P. syringae pv. aptata per kg soil were inoculated in cultivar Rosy Red 555 and Ruby Queen while in Red Express 505, 4000 or more J2 of M. incognita or 20.0 mL or more P. syringae pv. aptata caused significant reductions. A significant increase in proline in Rosy Red 555 and Ruby Queen was observed when 2000 or more J2 of M. incognita or 10.0 mL or more P. syringae pv. aptata were inoculated while in Red Express 505, 4000 or more J2 of M. incognita or 20 mL or more P. syringae pv. aptata caused significant increase. Galling and nematode population was increased with the increase in inoculum level of M. incognita. Bacterial leaf spot indices caused by P. syringae pv. aptata was also increased with the increase in inoculum level of bacterial pathogen. Leaf spot indices were recorded 1–5 scale in different cultivars.
Abstract
This work aimed to investigate interactions between antibody-opsonized SARS-CoV-2 and monocytes enriched from human peripheral blood mononuclear cells (PBMC) to determine whether antibody dependent enhancement might contribute to the pathophysiology of COVID-19. Pre-incubation of SARS-CoV-2 with sera from hospitalized COVID-19 patients led to significantly increased virus uptake and viral replication in monocytes. Remarkably, SARS-CoV-2 pre-incubated with sera from patients with severe COVID-19 but not those with mild disease or post vaccination strongly increased IL-6 secretion by monocytes. Antibody dependent viral uptake was partially inhibited by monoclonal anti-FcγRIIa antibody whereas IL-6 secretion was completely abolished. FcγRIIa preferentially binds IgG2, and sera from patients with severe COVID-19 contained lower IgG2 levels as compared to mild COVID-19 cases whereas IgG1 levels were increased. These data suggests that FcγRIIa-mediated binding of antibody-opsonized SARS-CoV-2 critically impacts monocytic inflammatory cytokine release and COVID-19 pathophysiology.
Abstract
Pochazia shantungensis (Chou & Lu, 1977), an alien species of Asian origin is reported from Hungary as well as from the Carpathian Basin for the first time as the first representative of the family Ricaniidae. The current distribution of the species is provided. The species may pose a potentially serious phytosanitary risk in the countries where it had been established. The Hungarian status of the species is dubious; however, it can possibly become established in the Hungarian fauna.
Abstract
Allium is a plant genus with high medicinal and dietary value, which encompasses commonly used culinary herbs such as scallion, garlic, and onion. In this study, we investigated whether Allium extracts exert protective effects on radiation-induced chromosomal damage in BALB/c mice. The mice were orally administered 200 mg kg−1 Allium extracts (scallion, garlic, and onion) for 6 weeks and exposed to 2 Gy gamma radiation 1 day after the final feeding. Haematological parameters and the number of micronucleated bone marrow polychromatic erythrocytes (MnPCEs) were evaluated. Additionally, we analysed the effects of Allium extracts on inflammasome activation in lipopolysaccharide-primed murine bone marrow-derived macrophages. The Allium extracts increased the numbers of peripheral white blood cells, lymphocytes, neutrophils, and red blood cells; significantly decreased the number of MnPCEs; and suppressed the maturation of interleukin-1β by blocking activation of the NLRP3 and AIM2 inflammasomes.
In conclusion, Allium extracts protected the hematopoietic function of bone marrow, reduced radiation-induced formation of micronuclei, and suppressed activation of the NLRP3 and AIM2 inflammasomes. Further research is needed to explore the molecular mechanisms of these effects of Allium.
Abstract
The aims of this study were to formulate 2% and 3% (w/v) hemp seed beverages and to evaluate chemical composition, colloidal stability, safety, and sensory profiles after roasting the seeds (150 °C, 15 min). Beverages with 2% and 3% roasted hemp seeds evidenced acceptable sensory attributes and nutritious profile, containing 0.6–1% protein, 0.4–0.6% dietary fibre, and 0.5–0.8% lipids –mainly unsaturated fatty acids–, respectively. The roasting treatment significantly increased the phenolic content by 49–55% and the antioxidant capacity by 15%, and improved the sensory attributes of the beverages. Low peroxide levels (<1 meq O2/kg) in roasted hemp seed beverages were detected up to five days in cold storage, with better capacity against oxidation at 2% seed content. The use of 0.03% gellan gum improved the physical stability of suspensions contributing to their overall acceptability. Roasted hemp seed beverages showed no cannabinoid and tetrahydrocannabinol (THC) content. The advantages observed after roasting the seeds on phenolic content, antioxidant activity, sensorial acceptability and safety provided better attributes for the feasibility of beverages formulated with hemp seeds. The results showed a formulation suitable for the development of potential industrial roasted hemp seeds beverages with promising compositional profiles.
Abstract
The automated system for enhancing plant growth presents an innovative approach to optimize quality of sugarcane cultivation for four main sugarcane growing zones. It includes issues like recommendation of crops based on soil nutrients, diagnosis of disease in the leaf and stem images of sugarcane, weed detection and harvesting time prediction. The research work proposed in the article presents an innovative two-stage approach for object detection and classification in agricultural imagery. Initially, YOLOv8 (You Only Look Once) is employed to accurately detect objects within images, delineating them with precise boundary boxes. Subsequently, the focus of hybrid model integrating Convolutional Neural Networks (CNNs) and Long Short-Term Memory (LSTM) networks, known as Contextual Long Short-Term Memory (CLSTM), is employed. This dual-stage methodology harnesses the speed and accuracy of YOLOv8 for robust object localization, while the CLSTM model ensures nuanced classification, contributing to comprehensive and accurate approach for object detection and crop-weed differentiation in agricultural scenarios. The proposed approach is compared with the four DL algorithms for identifying weeds in sugarcane crops and subsequently assessed their accuracy and F1 score performance. At a learning rate of 0.002, the findings of CLSTM showcase superior precision at 98.5%, recall at 97.8%, F1 score at 98.1%, and an overall accuracy of 97.7%. The subsequent task is harvesting time prediction, which entails identifying the best time to harvest sugarcane based on the planting period, weather predictions, and sugarcane brix value. The implementation of this automated system not only enhances the productivity of sugarcane cultivation but also serves as a model for sustainable and resource-efficient agriculture.
Abstract
Extracts from Azadirachta indica, Lantana camara, Nerium oleander, Aegle marmelos, Allium sativum, Citrus limon were compared with two checks viz. Azadirachtin 1 EC and Acephate 95 SG for their efficacy against Nilaparvata lugens, Orseolia oryzae and natural enemies in cultivated rice during two consecutive wet seasons from 2022 to 2023. It was revealed that, mean rice yield was significantly higher in the A. indica and Acephate 95 SG treatments compared to the control. The A. indica treatment emerged as the best treatment against O. oryzae whereas, the A. marmelos treatment was found most promising against N. lugens. All the studied botanicals had less impact on natural enemies than synthetic chemical. The cost economics of major insect pests' management in rice revealed that Acephate 95 SG treatment recorded the highest Incremental Cost Benefit Ratio (1: 4.65) followed by A. indica (1: 3.74) and A. marmelos (1: 3.41).
40 accessions of Aegilops tauschii Coss. specially chosen to present as much of the species variability as possible in such sample were used to build a phylogenetic tree based on transcriptomes sequences. The tree displays subdivision of Ae. tauschii into TauL1, TauL2 and TauL3 gene-pools, and also into subsp. tauschii (TauL1) and subsp. strangulata (TauL2 + TauL3). TauL3 does not look as an outlier. Both subsp. strangulata and subsp. tauschii are highly variable. Accessions from all clades of subsp. tauschii and some clades of subsp. strangulata have disjoint patterns of geographic occurrence, while some of subsp. strangulata clades correspond to particular geographical regions. No special separate clade was formed by some accessions of Ae. tauschii subsp. tauschii from Yellow River region.
Abstract
Thirty nine species of oribatid mites (Oribatida), belonging to 29 genera and 20 families were collected from semidecayed leaves in the San Gabriel Limestone Cave, Dominican Republic. A new species of the genus Pergalumna (Galumnidae)—P. (Pergalumna) sangabrielensis n. sp.—is described.
A comprehensive lichenological survey was conducted on three Azorean islands: Faial, São Miguel, and Terceira, resulting in the documentation of 155 lichens and lichenicolous fungi species, with a total of 406 observations. Among these, 33 species are reported for the first time in the Archipelago, with four species representing new records for Europe (Lecanora subimmersa, Lecanora thysanota, Pseudosagedia cestrensis, and Traponora asterella). Additionally, 15 species and varieties are new to Portugal, including notable taxa such as Caloplaca interna, Lecanora gisleriana, Lecanora handelii, Variospora dolomiticola, and Xanthocarpia diffusa.
Salsola is one of the largest genera in the tribe Salsoleae within Amaranthaceae, with many species that share morphological characteristics, which makes it a challenging genus to study. In this study, 11 quantitative and eight qualitative morphological traits were evaluated and measured. Ward’s dendrogram showed two main clusters: one composed of S. brachiata and S. turcomanica, and the other composed of two subclusters, with populations of S. kali, S. incanescens, S. orientalis and S. dendroides. In addition, we performed molecular phylogenetic analyses conducted with sequence data from nrDNA (ITS and ETS) and cpDNA markers (psbB-psbH and atpB-rbcL) under maximum parsimony, Bayesian inference and maximum likelihood approaches. All the members were clustered into a well-supported clade (PP = 1, ML/BS = 0.89) composed of two subclades: subclade A included S. turcomanica and S. brachiata (PP = 0.90, ML/BS = 73) and subclade B (PP = 0.95, ML/BS = 81) comprised S. incanescens, S. orientalis, S. dendroides and S. kali. The current study provides novel insights at morphological and molecular levels, as well as the results of molecular studies based on nr- and cpDNA sequence data that are congruent with morphological analyses.
Two species new to science, Xanthoria pedersenii and X. wennergrenii in the Xanthoria calcicola complex (Xanthoria, Xanthorioideae, Teloschistaceae), characterized by a complex of morphological, anatomical and molecular characters, are described, illustrated and compared with closely related taxa.
New Records for the Bryoflora of Vietnam, 6.
Bazzania tranninhiana sp. nov. (Lepidoziaceae)
During our visit to the Tam Đảo National Park in 1998, with the guidance of Prof. Trần Ninh, we collected a peculiar Bazzania species unknown from Vietnam before. This species, with its incurved, fragile leaf apices and deeply dissected underleaves proved to be new to science. This paper provides its illustrated description.
Peltula shalae Kumar S. is described as a new species of lichens from the Chenab Valley of Jammu and Kashmir, India. It is characterised by peltate-umbilicate thallus comprising of overlapping squamules forming rosettes, with diameter 2–3.5 cm. The absence of soredia is notable. This lichen is observed to grow on ‘shale rock’, a type of sedimentary rock, and is distinguished from closely related species, including P. daurica, P. sonorensis, P. anthracina, and P. obscurans by its distinct morphological features. Furthermore, a comprehensive comparison with closely related peltate-umbilicate species is presented.
Plasmodium falciparum is primarily transmitted by Anopheles gambiae. Malaria caused by Plasmodium falciparum is a major public health issue in western Kenya and sub-Saharan Africa, accounting for 90% of malaria deaths. The primary methods of malaria prevention are indoor residual spraying and the use of insecticide-treated nets. These tools face challenges such as mosquito resistance to insecticides as well as their toxic effect to the non-target organism, therefore this study aims to explore the application of lichen secondary metabolites as potential oral biological insecticides by assessing mosquito mortality in varying concentrations. Lichen secondary metabolites were extracted from Cladonia foliacea thalli. Bioassay experiments were conducted on A. gambiae Kisumu strain mosquitoes. Mortality rates were measured after ingesting sugar bait and lichen extracts in different concentrations. Three test replicates and negative control were used, with mortality measured after 4, 24, 48, and 72 hours. Analysis using three-way analysis of variance with twoway interactions was performed using R program to determine the effect of different lichen extract concentrations, time of exposures and mosquito sex on mortality. Our results showed that the ingestion of C. foliacea extract at 50 mg/ml and a post-exposure period of 24 to 48 hours had a maximum effect on the mortality rate of targeted male and female A. gambiae. No statistical difference was found between male and female mosquitoes in mortality. Our study confirms firstly that the extract of C. foliacea is a promising oral toxic agent against adult malaria vector A. gambiae.
Moroccan oases host a rich array of date palm varieties, renowned for the traditional expertise in crafting local products from dates. Date juice, a quintessential local product with significant heritage value, is facing a decline in appreciation. This study delves into indigenous knowledge surrounding date juice processing. Surveys involving one hundred women from the Alnif and Tata oases in Morocco’s pre-Saharan regions were conducted. Results reveal the use of 56 medicinal and aromatic plants from 33 families and 53 genera in date juice preparation, with Lamiaceae and Asteraceae families most prevalent. Noteworthy species include Corrigiola telephiifolia Pourr. (85 citations), Cynara humilis L. (66), Cyperus longus L. (65), and Alpinia officinarum Hance (64). Date juice holds significance in traditional medicine, prized for its sensory qualities and therapeutic benefits resulting from blending date fruit with aromatic and medicinal plants. The study sheds light on traditional manufacturing processes, including palm variety selection and plant choices. It also offers insights into therapeutic applications aligning with medicinal practices. These findings enrich understanding of cultural traditions and provide essential information for their preservation and propagation.
Ecological restoration requires large-scale reintroductions of plants, but their genetic basis is a controversial issue. Formerly, non-local seed sourcing of naturally occurring herbaceous species was common practice. Here we test whether the genetic pattern of the earlier introduction of non-local seeds of Leucanthemum vulgare agg. (ox-eye daisy) can still be detected several years after the application and whether it differs from that of the regional gene pool. We collected leaf material of the ox-eye daisy in Central Germany on sites of indigenous populations (I) and those formerly restored with non-local seed sources (R). Genome sizes and population genetic pattern (AFLP) were analysed. Genome size estimates of most of the individuals studied suggest, that most ox-eye daisies in the region have similar genome sizes regardless of their origin, while individuals from two indigenous populations from the most northwestern part of the study area had lower 1C values. All populations were genetically diverse and the former use of non-local geno-types of the species could not be detected up to more than 8 years after the establishment of the populations. The results shows that a recommendation for restoration purposes is unequivocal, it can only be concluded that it will be best to use seeds that are local and/or similar to the sites intended for sowing.
The new species Verrucaria aptrootii sp. nov. is described from Darel Valley, Gilgit Baltistan, Pakistan. A comparative morpho-anatomical, chemical study and ITS-based molecular analyses confirmed the position of this new species within the genus Verrucaria. Verrucaria aptrootii sp. nov. is closely related to V. squamulosa and characterized by a not squamulose thallus, plane to concave areoles, excipulum of 25–40 µm, simple, branched periphyses, ascospores of 27–35 × 10–15 µm and calcareous rock substrate.
Abstract
Introduction
This study was conducted to determine the specific features of the mutual influence of the immune and haemostatic systems in immunohaemostasis, the role of immune reactions in the regulation of blood coagulation, and the efficacy of modern methods of treating thrombosis and bleeding.
Methods
The study analysed relevant scientific sources on immunology and haematology and identified the specific features of the blood clotting process and the role of immune reactions in it.
Results
The study found that the immune system influences the haematological system through the interaction of blood clotting factors, platelets, plasminogen, endothelial cells with immune cells. The haemostatic system influences the immune system through mechanisms to maintain immune tolerance and immune memory and the properties of clotting factors to activate the stimulation and migration of immune cells to the site of infection. Immune reactions regulate blood coagulation by activating platelets, regulating blood coagulation factors, affecting fibrinolysis, and immune tolerance. The process of platelet activation involves immune cells, immune complexes, and microbial components. The regulation of blood coagulation factors is influenced by the ability of immune cells to produce activators and inhibitors of these factors and to stimulate or slow down fibrinolysis. The immune system's maintenance of immune tolerance to blood components is regulated by mechanisms of immune response suppression, partial immune ignoring of certain blood elements, inhibition of activation of certain immune cells, apoptosis, and selection of immature T-lymphocytes. Treatment methods for patients at risk of thrombosis and bleeding include anticoagulation, antiplatelet, dual antiplatelet therapy, thrombectomy, endovascular methods, medical prophylaxis of bleeding, and coagulation monitoring.
Conclusions
The findings of this study suggest the significance of immune responses in the regulation of blood coagulation processes, and therefore they can be used in the development of immunotherapy methods for the treatment of thrombosis and bleeding.
