View More View Less
  • 1 Hungarian Academy of Sciences, P.O. Box 18, H-1581 Budapest, Hungary
Open access

The antimicrobial susceptibility of 19 Bordetella avium and 36 Ornithobacterium rhinotracheale strains was tested by the Kirby-Bauer disk diffusion method, and the minimal inhibitory concentrations (MIC) of amoxicillin, doxycycline and erythromycin were also determined. Most O. rhinotracheale strains were resistant to nalidixic acid, sulphamethoxazole–trimethoprim and gentamicin, and were susceptible to ampicillin, chloramphenicol, spectinomycin and tilmicosin. All B. avium strains were resistant to ceftiofur and lincomycin and susceptible to doxycycline, gentamicin, polymyxin B, spectinomycin and sulphonamides. The MICs ranged widely for all three antibiotics tested against O. rhinotracheale strains, from 0.12 μg/ml to 32 μg/ml for amoxicillin and erythromycin, and from 0.6 μg/ml to 32 μg/ml for doxycycline. For B. avium isolates, the MIC values ranged from ≤ 0.03 μg/ml to 1 μg/ml for amoxicillin, from ≤ 0.03 μg/ml to 0.12 μg/ml for doxycycline and from 8 μg/ml to 16 μg/ml for erythromycin. These findings support the idea that the use of antibiotics in a region or a farm may affect antimicrobial resistance and underline the need for prudent application of antibiotic therapy based on proper antimicrobial susceptibility testing.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Ak, S.and Turan, N. (2001): Antimicrobial susceptibility of Ornithobacterium rhinotracheale isolated from broiler chickens in Turkey. Veterinarski Arhiv 71, 1213.

    • Search Google Scholar
    • Export Citation
  • Beach, N. M., Thompson, S., Mutnick, R., Brown, L., Kettig, G., Puffenbarger, R., Stockwell, S. B., Miyamoto, D.and Temple, L. (2012): Bordetella avium antibiotic resistance, novel enrichment culture, and antigenic characterization. Vet. Microbiol. 160, 1893.

    • Search Google Scholar
    • Export Citation
  • Clinical and Laboratory Standards Institute (formerly: National Committee for Clinical Laboratory Standards) (2002): Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals: Approved Standard. Second edition. NCCLS document M31-A2. National Committee for Clinical Laboratory Standards, Wayne, PA.

    • Search Google Scholar
    • Export Citation
  • Clinical and Laboratory Standards Institute (2004): Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals: Informational Supplement. CLSI document M31-S1. Clinical and Laboratory Standards Institute, Wayne, PA. Clinical and Laboratory Standards Institute (2011): Performance Standards for Antimicrobial Susceptibility Testing: Twenty-first Informational Supplement. CLSI document M100-S21. Clinical and Laboratory Standards Institute, Wayne, PA.

  • Devriese, L. A., De Herdt, P.and Haesebrouck, F. (2001): Antibiotic sensitivity and resistance in Ornithobacterium rhinotracheale strains from Belgian broiler chickens. Avian Pathol. 30, 1973.

    • Search Google Scholar
    • Export Citation
  • Malik, Y. S., Olsen, K., Kumar, K.and Goyal, S. M. (2003a): In vitro antibiotic resistance profiles of Ornithobacterium rhinotracheale strains isolated from Minnesota turkeys during 1996–2002. Avian Dis. 47, 5883.

    • Search Google Scholar
    • Export Citation
  • Malik, Y. S., Olsen, K., Chander, Y.and Goyal, S. M. (2003b): Antimicrobial resistance in bacterial pathogens isolated from turkeys in Minnesota from 1998 to 2002. Int. J. Appl. Res. Vet. Med. 14, 5063.

    • Search Google Scholar
    • Export Citation
  • Malik, Y. S., Chander, Y., Gupta, S. C.and Goyal, S. M. (2005): A retrospective study on antimicrobial resistance in Mannheimia (Pasteurella) haemolytica, Escherichia coli, Salmonella species, and Bordetella avium from chickens in Minnesota. J. Appl. Poult. Res. 14, 5063.

    • Search Google Scholar
    • Export Citation
  • Mohd-Zain, Z., Lin Jee, T.and Jusoff, K. (2008): Phenotypic characteristics, antibiotic susceptibility and pathogenicity of Ornithobacterium rhinotracheale. WSEAS Transact. Biol. Biomed. 7, 1333.

    • Search Google Scholar
    • Export Citation
  • Mortensen, J. E., Brumbach, A.and Shryock, T. R. (1989): Antimicrobial susceptibility of Bordetella avium and Bordetella bronchiseptica isolates. Antimicrob. Agents Chemother. 33, 7713.

    • Search Google Scholar
    • Export Citation
  • Murthy, T. R. K. G., Dorairajan, N., Balasubramanium, G. A., Dinakaran, A. M.and Saravanabava, K. (2008): In vitro antibiotic sensitivity of Ornithobacterium rhinotracheale strains isolated from laying hens in India. Vet. Arhiv 78, 493.

    • Search Google Scholar
    • Export Citation
  • Raffel, T. R., Register, K. B., Marks, S. A.and Temple, L. (2002): Prevalence of Bordetella avium infection in selected wild and domesticated birds in the Eastern USA. J. Wildl. Dis. 38, 403.

    • Search Google Scholar
    • Export Citation
  • Register, K. B.and Yersin, G. (2005): Analytical verification of a PCR assay for identification of Bordetella avium. J. Clin. Microbiol. 43, 55673.

    • Search Google Scholar
    • Export Citation
  • Soriano, V. E., Vera, N. A., Salado, C. R., Fernández, R. P. and Blackall, P. J. (2003): In vitro susceptibility of Ornithobacterium rhinotracheale to several antimicrobial drugs. Avian Dis. 47, 4763.

    • Search Google Scholar
    • Export Citation
  • Tanyi, J., Bistyák, A., Kaszanyitzky, É., Vetési, F. and Dobos-Kovács, M. (1995): Isolation of Ornithobacterium rhinotracheale from chickens and turkeys with respiratory disease. Preliminary report [in Hungarian, with English abstract]. Magyar Állatorvosok Lapja 50, 3283.

    • Search Google Scholar
    • Export Citation
  • Tsai, H. J.and Huang, C. W. (2006): Phenotypic and molecular characterization of isolates of Ornithobacterium rhinotracheale from chickens and pigeons in Taiwan. Avian Dis. 50, 5023.

    • Search Google Scholar
    • Export Citation
  • van Empel, P. C. M. and Hafez, H. M. (1999): Ornithobacterium rhinotracheale: a review. Avian Pathol. 28, 2173.

  • van Veen, L., Hartman, E.and Fabri, T. (2001): In vitro antibiotic sensitivity of strains of Ornithobacterium rhinotracheale isolated in the Netherlands between 1996 and 1999. Vet. Rec. 149, 6113.

    • Search Google Scholar
    • Export Citation
  • Varga, J., Fodor, L.and Makrai, L. (2001): Characterisation of some Ornithobacterium rhinotracheale strains and examination of their transmission via eggs. Acta Vet. Hung. 49, 1253.

    • Search Google Scholar
    • Export Citation

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Aug 2020 0 9 11
Sep 2020 0 1 4
Oct 2020 0 1 4
Nov 2020 0 7 4
Dec 2020 0 3 4
Jan 2021 0 6 8
Feb 2021 0 0 0