View More View Less
  • 1 Hungarian Academy of Sciences, Budapest, Hungary
  • | 2 National Food Chain Safety Office, Budapest, Hungary
  • | 3 Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden
Open access

Sequence analysis revealed a short alternative open reading frame (ORF) named ORF7a within the nucleocapsid gene of genetically divergent porcine reproductive and respiratory syndrome virus (PRRSV) genomes. Alignment of the corresponding protein sequences (named 7ap) revealed substantial heterogeneity among 7aps of different genotypes, though all of them are predicted to be positively charged. Green fluorescent protein and FLAG fusion constructs of ORF7a of the HU-14432/2011 PRRSV demonstrated that 7ap is expressed. 7ap of HU- 14432/2011 (Hu7ap) was synthesised chemically, and ELISA experiments revealed that Hu7ap binds strongly to mammalian IgGs. Protein-protein gel retardation assays and complement fixation inhibition suggest that 7aps bind to the CH2 domain of the IgG(Fc) fragment. Cellular localisation and immunological characteristics of PRRSV 7ap may indicate multiple functions including nuclear and cytoplasmic over-tuning of normal cellular processes and immunosuppression.

  • Bergeron, J., Menezes, J. and Tijssen, P. (1993): Genomic organization and mapping of transcription and translation products of the NADL-2 strain of porcine parvovirus. Virology 197, 8698.

    • Search Google Scholar
    • Export Citation
  • Chang, C. C., Yoon, K. J., Zimmerman, J. J., Harmon, K. M., Dixon, P. M., Dvorak, C. M. and Murtaugh, M. P. (2002): Evolution of porcine reproductive and respiratory syndrome virus during sequential passages in pigs. J. Virol. 76, 47504763.

    • Search Google Scholar
    • Export Citation
  • Charerntantanakul, W. (2012): Porcine reproductive and respiratory syndrome virus vaccines: Immunogenicity, efficacy and safety aspects. World J. Virol. 12, 2330.

    • Search Google Scholar
    • Export Citation
  • Fang, Y., Treffers, E. E., Li, Y., Tas, A., Sun, Z., van der Meer, Y., de Ru, A. H., Van Veelen, P. A., Atkins, J. F., Snijder, E. J. and Firth, A. E. (2012): Efficient –2 frameshifting by mammalian ribosomes to synthesize an additional arterivirus protein. Proc. Natl Acad. Sci. U. S. A. 109, E2920E2928. doi: 10.1073/pnas.1211145109.

    • Search Google Scholar
    • Export Citation
  • Firth, A. E., Zevenhoven-Dobbe, J. C., Wills, N. M., Go, Y. Y., Balasuriya, U. B. R., Atkins, J. F., Snijder, E. J. and Posthuma, C. C. (2011): Discovery of a small arterivirus gene that over laps the GP5 coding sequence and is important for virus production. J. Gen. Virol. 92, 10971106.

    • Search Google Scholar
    • Export Citation
  • Fischer, F., Peng, D., Hingley, S. T., Weiss, S. R. and Masters, P. S. (1997): The internal open reading frame within the nucleocapsid gene of mouse hepatitis virus encodes a structural protein that is not essential for viral replication. J. Virol. 71, 9961003.

    • Search Google Scholar
    • Export Citation
  • Forsberg, R. (2005): Divergence time of porcine reproductive and respiratory syndrome virus subtypes. Mol. Biol. Evol. 22, 21312134.

  • Hanada, K., Suzuki, Y., Nakane, T., Hirose, O. and Gojobori, T. (2005): The origin and evolution of porcine reproductive and respiratory syndrome viruses. Mol. Biol. Evol. 22, 10241031.

    • Search Google Scholar
    • Export Citation
  • Hulo, C., de Castro, E., Masson, P., Bougueleret, L., Bairoch, A., Xenarios, I. and Le Mercier, P. (2011a): ViralZone: a knowledge resource to understand virus diversity. Nucleic Acids Res 39, D576582. doi: 10.1093/nar/gkq901. http://viralzone.expasy.org/all_by_protein/811.html (April 4, 2015.

    • Search Google Scholar
    • Export Citation
  • Hulo, C., de Castro, E., Masson, P., Bougueleret, L., Bairoch, A., Xenarios, I. and Le Mercier, P. (2011b): ViralZone: a knowledge resource to understand virus diversity. Nucleic Acids Res 39:D576582. doi: 10.1093/nar/gkq901. http://viralzone.expasy.org/all_by_species/812.html#tab6 (April 4, 2015).

    • Search Google Scholar
    • Export Citation
  • Jenkins, G. M., Rambaut, A., Pybus, O. G. and Holmes, E. C. (2002): Rates of molecular evolution in RNA viruses: a quantitative phylogenetic analysis. J. Mol. Evol. 54, 156165.

    • Search Google Scholar
    • Export Citation
  • Kim, H. S., Kwang, J., Yoon, I. J., Joo, H. S. and Frey, M. L. (1993): Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch. Virol. 133, 477483.

    • Search Google Scholar
    • Export Citation
  • Kiraga, J., Mackiewicz, P., Mackiewicz, D., Kowalczuk, M., Biecek, P., Polak, N., Smolarczyk, K., Dudek, M. R. and Cebrat, S. (2007): The relationships between the isoelectric point and: length of proteins, taxonomy and ecology of organisms. BMC Genomics 8, 163.

    • Search Google Scholar
    • Export Citation
  • Lopez, O. J. and Osorio, F. A. (2004): Role of neutralizing antibodies in PRRSV protective immunity. Vet. Immunol. Immunopathol. 102, 155163.

    • Search Google Scholar
    • Export Citation
  • Lunney, J. K., Fang, Y., Ladinig, A., Chen, N., Li, Y., Rowland, B. and Renukaradhya, G. J. (2016): Porcine reproductive and respiratory syndrome virus (PRRSV): Pathogenesis and interaction with the immune system Annu. Rev. Anim. Biosci. 4, 129154.

    • Search Google Scholar
    • Export Citation
  • Marqués, G., Antón, L. C., Barrio, E., Sánchez, A., Ruiz, S., Gavilanes, F. and Vivanco, F. (1993): Arginine residues of the globular regions of human C1q involved in the interaction with immunoglobulin G. J. Biol. Chem. 268, 1039310402.

    • Search Google Scholar
    • Export Citation
  • Mateu, E. and Diaz, I. (2008): The challenge of PRRS immunology. Vet. J. 177, 345351.

  • Meier, C., Aricescu, A. R., Assenberg, R., Aplin, R. T., Gilbert, R. J., Grimes, J. M. and Stuart, D. I. (2006): The crystal structure of ORF-9b, a lipid binding protein from the SARS coronavirus. Structure 14, 11571165.

    • Search Google Scholar
    • Export Citation
  • Murtaugh, M. P., Stadejek, T., Abrahante, J. E., Lam, T. T. and Leung, F. C. (2010): The everexpanding diversity of porcine reproductive and respiratory syndrome virus. Virus Res. 154, 1830.

    • Search Google Scholar
    • Export Citation
  • Papworth, C., Bauer, J. C., Braman, J. and Wright, D. A. (1996): Site-directed mutagenesis in one day with 80% efficiency. Strategies 9, 34.

    • Search Google Scholar
    • Export Citation
  • Park, S. H. and Raines, R. T. (2004): Fluorescence gel retardation assay to detect protein–protein interactions. Methods Mol. Biol. 261, 155160.

    • Search Google Scholar
    • Export Citation
  • Samrat, S. K., Li, W., Singh, S., Kumar, R. and Agrawal, B. (2014): Alternate reading frame protein (F protein) of hepatitis C virus: paradoxical effects of activation and apoptosis on human dendritic cells lead to stimulation of T cells. PLoS One 9, e86567. doi: 10.1371/journal.pone.0086567.

    • Search Google Scholar
    • Export Citation
  • Schneider, S. and Zacharias, M. (2012): Atomic resolution model of the antibody Fc interaction with the complement C1q component. Mol. Immunol. 51, 6672.

    • Search Google Scholar
    • Export Citation
  • Schwartz, R., Ting, C. S. and King, J. (2001): Whole proteome pI values correlate with subcellular localizations of proteins for organisms within the three domains of life. Genome Res. 11, 703709.

    • Search Google Scholar
    • Export Citation
  • Senanayake, S. D. and Brian, D. A. (1997): Bovine coronavirus I protein synthesis follows ribosomal scanning on the bicistronic N mRNA. Virus Res. 48, 101105.

    • Search Google Scholar
    • Export Citation
  • Shi, M., Lam, T. T. Y., Hon, C. C., Murtaugh, M. P., Davies, P. R., Hui, R. K. H., Li, J., Wong, L. T. W., Yip, C. W., Jiang, J. W. and Leung, F. C. C. (2010): Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses. J. Virol. 84, 87008711.

  • Stadejek, T., Oleksiewicz, M. B., Potapchuk, D. and Podgórska, K. (2006): Porcine reproductive and respiratory syndrome virus strains of exceptional diversity in eastern Europe support the definition of new genetic subtypes. J. Gen. Virol. 87, 18351841.

    • Search Google Scholar
    • Export Citation
  • Tatusov, T. and Tatusov, R. NCBI ORF Finder. http://www.ncbi.nlm.nih.gov/projects/gorf/.

  • Tóth, R., Mészáros, I., Farsang, A. and Zádori, Z. (2016): Mechanisms of action of veterinary vaccine adjuvants (in Hungarian, with English abstract). Magy. Allatorvosok 138, 3146.

    • Search Google Scholar
    • Export Citation
  • Vitagliano, L., Fiume, G., Scognamiglio, P. L., Doti, N., Cannavò, R., Puca, A., Pedone, C., Scala, G., Quinto, I. and Marasco, D. (2011): Structural and functional insights into I?B-a/HIV-1 Tat interaction. Biochimie 93, 15921600.

    • Search Google Scholar
    • Export Citation
  • Wu, W. H., Fang, Y., Farwell, R., Steffen-Bien, M., Rowland, R. R. R., Christopher-Hennings, J. and Nelson, E. A. (2001): 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virol. 287, 183189.

    • Search Google Scholar
    • Export Citation
  • Xu, X., Yu, X., Deng, X., Yue, M., Zhang, J., Zhu, D., Zhou, Z., Zhai, X., Xu, K. and Zhang, Y. (2014): Hepatitis C virus alternate reading frame protein decreases interferon-a secretion in peripheral blood mononuclear cells. Mol. Med. Rep. 9, 730736.

    • Search Google Scholar
    • Export Citation
  • Yu, D., Lv, J., Sun, Z., Zheng, H., Lu, J. and Yuan, S. (2009): Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus. Virol. 383, 2231.

    • Search Google Scholar
    • Export Citation
  • Zádori, Z., Szelei, J., Lacoste, M. C., Li, Y., Gariépy, S., Raymond, P., Allaire, M., Nabi, I. R. and Tijssen, P. (2001): A viral phospholipase A2 is required for parvovirus infectivity. Dev. Cell 1, 291302.

    • Search Google Scholar
    • Export Citation
  • Zuckermann, F. A., Garcia, E. A., Luque, I. D., Christopher-Hennings, J., Doster, A., Brito, M. and Osorio, F. (2007): Assessment of the efficacy of commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines based on measurement of serologic response, frequency of gamma-IFN-producing cells and virological parameters of protection upon challenge. Vet. Microbiol. 123, 6985.

    • Search Google Scholar
    • Export Citation

Author information is available in PDF.
Please, download the file from HERE.

The manuscript preparation instructions is available in PDF.
Please, download the file from HERE.

Senior editors

Editor-in-Chief: Mária BENKŐ

Managing Editor: András SZÉKELY

Editorial Board

  • Béla DÉNES (National Food Chain Safety Office, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
submission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

Acta Veterinaria Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription fee 2021 Online subsscription: 696 EUR / 872 USD
Print + online subscription: 804 EUR / 1004 USD
Subscription fee 2022 Online subsscription: 710 EUR / 892 USD
Print + online subscription: 824 EUR / 1028 USD
Subscription Information Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Publication
Programme
2020 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2021 0 2 2
Jul 2021 0 3 9
Aug 2021 0 6 3
Sep 2021 0 26 17
Oct 2021 0 16 11
Nov 2021 0 11 11
Dec 2021 0 1 2