View More View Less
  • 1 University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary
  • | 2 University of Veterinary Medicine, István u. 2, H-1078 Budapest, Hungary
  • | 3 National Public Health Centre, Budapest, Hungary
Open access

Twenty-two serum samples of healthy bitches were tested with the frozen and lyophilised version of the same ELISA kit (Quanticheck, Faculty of Veterinary Science, Budapest, Hungary). Samples were chosen on the basis of their progesterone (P4) concentrations, which were between 1.00 and 20.00 ng/mL. As it is well known, this range has the highest clinical relevance in ovulation diagnosis. Both types of microplates were read at 15-min intervals from the 15th until the 90th minute (min) of incubation, and the results were compared with those of frozen plates at 60 min of incubation as 100 percent. Lyophilised microplates gave on average 18 percent higher results than the frozen version at equal incubation times. The highest difference between lyophilised and frozen samples was observed at 45 and 60 min of incubation. Ninety-four percent of the reaction in the frozen microplate occurred in the first 15 min, and during the subsequent 30 min the reaction seemingly stopped. After the 45th min of incubation, this 94 percent increased to 108 percent in the subsequent 30 min, which remained the final approximate result at the end of the 90 min of incubation. In contrast to the frozen microplate, the measured concentration increased continuously in the lyophilised version and reached the highest level at the 60th min. The results of the lyophilised microplate reached the same level at 30 min of incubation as those of the frozen version at 60 min. In conclusion, a mechanical increase or decrease of the incubation time does not generate a linear change in the test results. This study demonstrated that the results of a series of samples collected from the same bitch cannot be compared if they are measured with different laboratory methods or different ELISA kits.

  • Chapwanya, A., Clegg, T., Stanley, P. and Vaughan, L. (2008): Comparison of the Immulite and RIA assay methods for measuring peripheral blood progesterone levels in Greyhound bitches.. Theriogenology 70, 795799.

    • Search Google Scholar
    • Export Citation
  • Concannon, P. W. (2011): Reproductive cycles of domestic bitch.. Anim. Reprod. Sci. 124, 200210.

  • Crowther, J. R. (Ed.) (1995): ELISA Theory and Practice. Humana Press Inc., Totowa, New Jersey, USA.

  • Henning, D. and Nielsen, K. (1987): Peroxidase-labelled monoclonal antibodies for use in enzyme immunoassay.. J. Immunoassay 8, 297307.

    • Search Google Scholar
    • Export Citation
  • Hock, B., Rahman, M., Rauchalles, S., Dankwardt, A., Seifert, M., Haindl, S. and Kramer, K. (1999): Stabilisation of immunoassays and receptor assays.. J. Mol. Catal. B: Enzym. 7, 115124.

    • Search Google Scholar
    • Export Citation
  • Molnár, L., Ács, J., Csernus, V., Solti, L., Varró, R. and Huszenicza, Gy. (1984): Enzyme immunoassay (EIA) of progesterone. Proc. of the Symp. on the Analysis of Steroids, Szeged, Hungary.

    • Search Google Scholar
    • Export Citation
  • Munro, C. and Stabenfeldt, G. (1984): Development of a microtitre microplate enzyme immunoassay for the determination of progesterone.. J. Endocrinol. 10, 4149.

    • Search Google Scholar
    • Export Citation
  • Navarro, H., Zarco, L., Ducoing, A., Flores, G. and Valencia, J. (1990): Effect of time and temperature of incubation of heparinized caprine blood on concentrations of progesterone detected in plasma.. Theriogenology 33, 749755.

    • Search Google Scholar
    • Export Citation
  • Schumacher, M., Mattern, C., Ghoumari, A., Oudinet, J. P., Liere, P., Labombarda, F., Sitruk-Ware, R., De Nicola, A. F. and Guennoun, R. (2014): Revisiting the roles of progesterone and allopregnanolone in the nervous system: Resurgence of the progesterone receptors.. Prog. Neurobiol. 113, 639.

    • Search Google Scholar
    • Export Citation
  • Sibley, D. E. T., Ramsay, G., Lubrano, G. J. and Guilbault, G. G. (1993): Stability and reusability of enzyme-linked immunosorbent assay (ELISA) microplates.. Anal. Lett. 26, 16231634.

    • Search Google Scholar
    • Export Citation
  • Tahir, M. Z., Thoumire, S., Raffaelli, M., Grimard, B., Reynaud, K. and Chastant-Maillard, S. (2013): Effect of blood handling conditions on progesterone assay results obtained by chemiluminescence in the bitch.. Domest. Anim. Endocrin. 45, 141144.

    • Search Google Scholar
    • Export Citation
  • Thuróczy, J., Wölfling, A., Tibold, A., Balogh, L., Jánoki, Gy. A. and Solti, L. (2003): Effect of anticoagulants and sampling time on results of progesterone determination in canine blood samples.. Reprod. Domest. Anim. 38, 386389.

    • Search Google Scholar
    • Export Citation
  • Volkmann, D. H. (2006): The effects of storage time and temperature and anticoagulant on laboratory measurements of canine blood progesterone concentrations.. Theriogenology 66, 15831586.

    • Search Google Scholar
    • Export Citation

Author information is available in PDF.
Please, download the file from HERE.

The manuscript preparation instructions is available in PDF.
Please, download the file from HERE.

Senior editors

Editor-in-Chief: Mária BENKŐ

Managing Editor: András SZÉKELY

Editorial Board

  • Béla DÉNES (National Food Chain Safety Office, Budapest Hungary)
  • Edit ESZTERBAUER (Veterinary Medical Research Institute, Budapest, Hungary)
  • Hedvig FÉBEL (National Agricultural Innovation Centre, Herceghalom, Hungary)
  • László FODOR (University of Veterinary Medicine, Budapest, Hungary)
  • Balázs HARRACH (Veterinary Medical Research Institute, Budapest, Hungary)
  • Peter MASSÁNYI (Slovak University of Agriculture in Nitra, Nitra, Slovak Republic)
  • Béla NAGY (Veterinary Medical Research Institute, Budapest, Hungary)
  • Tibor NÉMETH (University of Veterinary Medicine, Budapest, Hungary)
  • Zsuzsanna NEOGRÁDY (University of Veterinary Medicine, Budapest, Hungary)
  • Alessandra PELAGALLI (University of Naples Federico II, Naples, Italy)
  • Kurt PFISTER (Ludwig-Maximilians-University of Munich, Munich, Germany)
  • László SOLTI (University of Veterinary Medicine, Budapest, Hungary)
  • József SZABÓ (University of Veterinary Medicine, Budapest, Hungary)
  • Péter VAJDOVICH (University of Veterinary Medicine, Budapest, Hungary)
  • János VARGA (University of Veterinary Medicine, Budapest, Hungary)
  • Štefan VILČEK (University of Veterinary Medicine in Kosice, Kosice, Slovak Republic)
  • Károly VÖRÖS (University of Veterinary Medicine, Budapest, Hungary)
  • Herbert WEISSENBÖCK (University of Veterinary Medicine, Vienna, Austria)
  • Attila ZSARNOVSZKY (Szent István University, Gödöllő, Hungary)

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

2020  
Total Cites 987
WoS
Journal
Impact Factor
0,955
Rank by Veterinary Sciences 101/146 (Q3)
Impact Factor  
Impact Factor 0,920
without
Journal Self Cites
5 Year 1,164
Impact Factor
Journal  0,57
Citation Indicator  
Rank by Journal  Veterinary Sciences 93/166 (Q3)
Citation Indicator   
Citable 49
Items
Total 49
Articles
Total 0
Reviews
Scimago 33
H-index
Scimago 0,395
Journal Rank
Scimago Veterinary (miscellaneous) Q2
Quartile Score  
Scopus 355/217=1,6
Scite Score  
Scopus General Veterinary 73/183 (Q2)
Scite Score Rank  
Scopus 0,565
SNIP  
Days from  145
sumbission  
to acceptance  
Days from  150
acceptance  
to publication  
Acceptance 19%
Rate

 

2019  
Total Cites
WoS
798
Impact Factor 0,991
Impact Factor
without
Journal Self Cites
0,897
5 Year
Impact Factor
1,092
Immediacy
Index
0,119
Citable
Items
59
Total
Articles
59
Total
Reviews
0
Cited
Half-Life
9,1
Citing
Half-Life
9,2
Eigenfactor
Score
0,00080
Article Influence
Score
0,253
% Articles
in
Citable Items
100,00
Normalized
Eigenfactor
0,09791
Average
IF
Percentile
42,606
Scimago
H-index
32
Scimago
Journal Rank
0,372
Scopus
Scite Score
335/213=1,6
Scopus
Scite Score Rank
General Veterinary 62/178 (Q2)
Scopus
SNIP
0,634
Acceptance
Rate
18%

 

Acta Veterinaria Hungarica
Publication Model Hybrid
Submission Fee none
Article Processing Charge 1100 EUR/article
Printed Color Illustrations 40 EUR (or 10 000 HUF) + VAT / piece
Regional discounts on country of the funding agency World Bank Lower-middle-income economies: 50%
World Bank Low-income economies: 100%
Further Discounts Editorial Board / Advisory Board members: 50%
Corresponding authors, affiliated to an EISZ member institution subscribing to the journal package of Akadémiai Kiadó: 100%
Subscription Information Online subsscription: 696 EUR / 872 USD
Print + online subscription: 804 EUR / 1004 USD
Online subscribers are entitled access to all back issues published by Akadémiai Kiadó for each title for the duration of the subscription, as well as Online First content for the subscribed content.
Purchase per Title Individual articles are sold on the displayed price.

Acta Veterinaria Hungarica
Language English
Size A4
Year of
Foundation
1951
Publication
Programme
2020 Volume 68
Volumes
per Year
1
Issues
per Year
4
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-6290 (Print)
ISSN 1588-2705 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2021 0 7 9
Mar 2021 0 2 5
Apr 2021 0 6 8
May 2021 0 5 9
Jun 2021 0 6 4
Jul 2021 0 3 0
Aug 2021 0 0 0