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  • 1 University of Pannonia, Deák F. u. 16, H-8360 Keszthely, Hungary
  • 2 Faculty of Veterinary Medicine, University of Helsinki, Saari Unit, Saarentaus, Finland
  • 3 Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Debrecen, Hungary
Open access

The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects. Therefore, we conclude that the assay could be incorporated into the laboratory routine where flow cytometry is applied for semen quality control.

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