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  • 1 Szent István University, Páter Károly u. 1, H-2103 Gödöllő, Hungary
  • 2 Szent István University, Páter Károly u. 1, H-2103 Gödöllő, Hungary
  • 3 Academy of Sciences—Kaposvár University, Kaposvár, Hungary
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The purpose of this study was to investigate the short-term effects of a single oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg−1 body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg−1 body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours. T-2 toxin did not cause changes in these parameters. Glutathione content and glutathione peroxidase activity showed higher levels at the 16th hour as the effect of both mycotoxins. The expression of glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) revealed a dual response. Downregulation was observed at the 8th hour, followed by an induction at the 16th hour, at the lowest dose of both mycotoxins. Higher doses revealed long-drawn emergence and an elevation was observed only at the 24th hour. However, at the lowest and highest doses of DON or T-2 toxin the changes in gene expression were delayed, which may be related to the low oxidative stress response, as suggested by the expression profiles of the nrf2, keap1, gpx4a and gpx4b genes.

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  • Anater, A., Manyes, L., Meca, G., Ferrer, E., Luciano, F. B., Pimpão, C. T. and Font, G. (2016): Mycotoxins and their consequences in aquaculture: A review. Aquaculture 451, 110.

    • Search Google Scholar
    • Export Citation
  • AOAC (1984): Official Methods of Analysis of the Association of Official Analytical Chemists (28.054). 14th edition. Arlington, VA, USA.

  • Balogh, K., Heincinger, M., Fodor, J. and Mézes, M. (2009): Effects of long term feeding of T-2 and HT-2 toxin contaminated diet on the glutathione redox status and lipid peroxidation processes in common carp (Cyprinus carpio L.). Acta Biol. Szegediensis 5, 2327.

    • Search Google Scholar
    • Export Citation
  • Binder, E. M., Tan, L. M., Chin, L. J., Handl, J. and Richard, J. (2007): Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients. Anim. Feed Sci. Technol, 137, 265282.

    • Search Google Scholar
    • Export Citation
  • Botsoglou, N. A., Fletouris, D. J., Papageorgiou, G. E., Vassilopoulos, V. N., Mantis, A. J. and Trakatellis, A. G. (1994): Rapid, sensitive and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food and feedstuff samples. J. Agric. Food Chem. 42, 19311937.

    • Search Google Scholar
    • Export Citation
  • Farkas, T., Csengeri, I., Majoros, F. and Oláh, J. (1980): Metabolism of fatty acids in fish: III. Combined effect of environmental temperature and diet on formation and deposition of fatty acids in the carp, Cyprinus carpio Linnaeus 1758. Aquaculture 20, 2940.

    • Search Google Scholar
    • Export Citation
  • Fodor, J., Németh, M., Kametler, L., Pósa, R., Kovács, M. and Horn, P. (2006): Novel methods of Fusarium toxins’ production for toxicological experiments. Acta Agr. Kaposváriensis 10, 277285.

    • Search Google Scholar
    • Export Citation
  • Fritz, K. S., Galligan, J. J., Hirschey, M. D., Verdin, E. and Petersen, D. R. (2012): Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice. J. Proteome Res. 11, 16331643.

    • Search Google Scholar
    • Export Citation
  • Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974): Glutathione S-transferases: The first enzymatic step in mercapturic c acid formation. J. Biol. Chem. 249, 71307139.

    • Search Google Scholar
    • Export Citation
  • Hermesz, E. and Ferencz, A. (2009): Identification of two phospholipid hydroperoxide glutathione peroxidase (gpx4) genes in common carp. Comp. Biochem. Physiol. C 150, 101106.

    • Search Google Scholar
    • Export Citation
  • Holladay, S. D., Smith, B. J. and Luster, M. I. (1995): B-lymphocyte precursor cells represent sensitive targets of T-2 mycotoxin exposure. Toxicol. Appl. Pharmacol. 131, 309315.

    • Search Google Scholar
    • Export Citation
  • Hooft, J. M., Elmor, A. E. H. I., Encarnação, P. and Bureau, D. P. (2011): Rainbow trout (Oncorhynchus mykiss) is extremely sensitive to the feed-borne Fusarium mycotoxin deoxynivalenol (DON). Aquaculture 311, 224232.

    • Search Google Scholar
    • Export Citation
  • Jiang, J., Shi, D., Zhou, X. Q., Yin, L., Feng, L., Liu, Y., Jiang, W. D. and Zhao, Y. (2015): Effects of glutamate on growth, antioxidant capacity, and antioxidant-related signaling molecule expression in primary cultures of fish enterocytes. Fish Physiol. Biochem. 41, 11431153.

    • Search Google Scholar
    • Export Citation
  • Kapoor, B. B., Smit, H. and Verighina, I. A. (1976): The alimentary canal and digestion in teleosts. Adv. Marine Biol. 13, 109239.

  • Kendziorski, C. M., Zhang, Y., Lan, H. and Attie, A. D. (2003): The efficiency of pooling mRNA in microarray experiments. Biostatistics 4, 465477.

    • Search Google Scholar
    • Export Citation
  • Kravchenko, L. V., Galash, V. T., Avreneva, L. T. and Kranauskas, A. E. (1989): On the sensitivity of carp, Cyprinus carpio, to mycotoxin T-2. J. Ichthyol. 29, 156160.

    • Search Google Scholar
    • Export Citation
  • Lawrence, R. A. and Burk, R. F. (1976): Glutathione peroxidase activity in selenium deficient rat liver. Biochem. Biophys. Res. Commun. 71, 952956.

    • Search Google Scholar
    • Export Citation
  • Livak, K. J. and Schmittgen, T. D. (2001): Analysis of relative gene expression data using realtime quantitative PCR and the 2(-DeltaDelta C(T)) method. Methods 25, 402408.

    • Search Google Scholar
    • Export Citation
  • Lowry, O. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. J. (1951): Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265275.

    • Search Google Scholar
    • Export Citation
  • Nel, A., Xia, T., Mädler, L. and Li, N. (2006): Toxic potential of materials at the nanolevel. Science 311, 622627.

  • Pelyhe, Cs. , Kövesi, B., Zándoki, E., Kovács, B., Szabó-Fodor, J., Mézes, M. and Balogh, K. (2016): Effect of 4-week feeding of deoxynivalenol or T-2-toxin-contaminated diet on lipid peroxidation and glutathione redox system in the hepatopancreas of common carp (Cyprinus carpio L.). Mycotox. Res. 32, 7783.

    • Search Google Scholar
    • Export Citation
  • Pietsch, C., Schulz, C., Pere Rovira, P., Kloas, W. and Burkhardt-Holm, P. (2014): Organ damage and hepatic lipid accumulation in carp (Cyprinus carpio L.) after feed-borne exposure to the mycotoxin, deoxynivalenol (DON). Toxins 6, 756778.

    • Search Google Scholar
    • Export Citation
  • Pietsch, C., Katzenback, B. A., Garcia-Garcia, E., Schulz, C., Belosevic, M. and Burkhardt-Holm, P. (2015): Acute and subchronic effects on immune responses of carp (Cyprinus carpio L.) after exposure to deoxynivalenol (DON) in feed. Mycotox. Res. 31, 151164.

    • Search Google Scholar
    • Export Citation
  • Poston, H. A., Coffin, J. L. and Combs, G. F. (1982): Biological effects of dietary T-2 toxins on rainbow trout, Salmo gairdneri. Aquat. Toxicol. 2, 7988.

    • Search Google Scholar
    • Export Citation
  • Pussemier, L., Piérard, J. Y., Anselme, M., Tangni, E. K., Motte, J. C. and Larondelle, Y. (2006): Development and application of analytical methods for the determination of mycotoxins in organic and conventional wheat. Food Addit. Contam. 23, 12081218.

    • Search Google Scholar
    • Export Citation
  • Rahman, I., Kode, A. and Biswas, S. K. (2007): Assay for quantitative determination of glutathione and glutathione disulphide levels using enzymatic recycling method. Nat. Prot. 1, 31593165.

    • Search Google Scholar
    • Export Citation
  • Šišperová, E., Modrá, H., Ziková, A., Kloas, W., Blahová, J., Matejová, I., Živná, D. and Svobodová, Z. (2015): The effect of mycotoxin deoxynivalenol (DON) on the oxidative stress markers in rainbow trout (Oncorhynchus mykiss, Walbaum 1792). J. Appl. Ichthyol. 31, 855861.

    • Search Google Scholar
    • Export Citation
  • Smith, I. K., Vierhelle, T. L. and Thorne, C. A. (1988): Assay of glutathione reductase in crude tissue homogenates using 5,5’-dithiobis(2-nitrobenzoic acid). Anal. Biochem, 175, 408413.

    • Search Google Scholar
    • Export Citation
  • Suzuki, T. and Yamamoto, M. (2015): Molecular basis of the Keap1–Nrf2 system. Free Radic. Biol. Med. 88, 93100.

  • Taguchi, K., Motohashi, H. and Yamamoto, M. (2011): Molecular mechanisms of the Keap1–Nrf2 pathway in stress response and cancer evolution. Genes Cells 16, 123140.

    • Search Google Scholar
    • Export Citation
  • Trebstein, A., Seefelder, W., Lauber, U. and Humpf, H. U. (2008): Determination of T-2 and HT-2 toxins in cereals including oats after immunoaffinity cleanup by liquid chromatography and fluorescence detection. J. Agric. Food Chem. 56, 49684975.

    • Search Google Scholar
    • Export Citation
  • Wang, L., Harris, S. M., Espinoza, H. M., McClain, V. and Gallagher, E. P. (2012): Characterization of phospholipid hydroperoxide glutathione metabolizing peroxidase (gpx4) isoforms in Coho salmon olfactory and liver tissues and their modulation by cadmium. Aquatic Toxicol. 114–115, 134141.

    • Search Google Scholar
    • Export Citation
  • Wulund, L. and Reddy, A. B. (2015): A brief history of circadian time: The emergence of redox oscillations as a novel component of biological rhythms. Perspect. Sci. 6, 2737.

    • Search Google Scholar
    • Export Citation

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