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  • 1 Hungarian Academy of Sciences, Hungária krt. 21, H-1143 Budapest, Hungary
  • 2 National Food Chain Safety Office, Budapest, Hungary
  • 3 Kallert & Loy GbR, Adelsdorf, Germany
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One of the main obstacles in freshwater aquaculture is the parasitic ciliate Ichthyophthirius multifiliis (Ich), the causative agent of white spot disease. The use of immunostimulants as feed additives may be a promising approach to control Ich infection. In the present study, we tested the prophylactic effect of orally administered β-1,3/1,6-glucan and propolis extract E50 against Ich infection in common carp. In total, 122 fish were separated into three experimental groups fed with a control, 3% β-glucan and 1% propolis diet for 40 consecutive days, respectively. On day 40, 16 fish per group were individually exposed to Ich theronts and the number of trophonts was counted 5 days post exposure. Relative gene expression of interleukin 1-β (IL-1-β) in common carp liver was examined by qPCR. Compared to control, the mean infection intensity was lower in the β-glucan- and propolis-fed groups; however, the difference was not statistically significant. The relative expression of IL-1-β significantly decreased in the propolis-fed group at day 10. In the β-glucan-fed group, a significant IL-1-β decrease was detected at day 15 compared to control. Although the Ich infection intensity was slightly decreased in both treated groups, and IL-1-β was moderately down-regulated in the liver of common carp, our results suggest that the applied feeding regime is insufficient to prevent Ich outbreaks in common carp.

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Editor(s)-in-Chief: Benkő, Mária

Managing Editor(s): Székely, András

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      Dénes, Béla
      Eszterbauer, Edit
      Fébel, Hedvig
      Fodor, László
      Harrach, Balázs
      Andras Komaromy (USA)
      Peter Massanyi (Slovak Republic)
      Nagy, Béla
      Németh, Tibor
      Neogrády, Zsuzsanna
      Kurt Pfister (Germany)
      Solti, László
      Szabó, József
      Vajdovich, Péter
      Varga, János
      Štefan, Vilĉek (Slovak Republic)
      Vörös, Károly
      Herbert Weissenböck (Austria)
      Zsarnovszky, Attila

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