View More View Less
  • 1 National Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Puławy, Poland
  • 2 National Veterinary Research Institute, 57 Partyzantów Avenue, 24-100 Puławy, Poland
  • 3 Animal and Plant Health Agency (Weybridge), Addlestone, Surrey, UK
  • 4 Medical University of Lublin, Lublin, Poland
Restricted access

Purchase article

USD  $25.00

1 year subscription (Individual Only)

USD  $836.00

Mycoplasma bovis is a primary infectious agent of many disorders in cattle including bovine respiratory disease. No commercial vaccines against M. bovis are available in Europe. The immune response of calves to three saponin-based adjuvants combined with a field Polish M. bovis strain was evaluated. Four groups of six calves each were injected subcutaneously with the M. bovis strain combined with either saponin, saponin + Emulsigen®, saponin + Emulsigen® + alphatocopherol acetate, or with phosphate-buffered saline as control group. Blood and nasal swab samples were collected up to day 84 post injection. All formulations effectively stimulated the humoral and the cellular immune response of the calves, but the course of the response depended on the adjuvant formulation. These immunological data provide additional information supporting the findings of previous M. bovis saponin and Emulsigen® vaccine challenge studies to facilitate the development of successful M. bovis vaccines.

  • Ayling, R. D., Baker, S. E., Peek, M. L., Simon, A. J. and Nicholas, R. A. J. (2000): Comparison of in vitro activity of danofloxacin, florfenicol, oxytetracycline, spectinomycin and tilmicosin against recent field isolates of Mycoplasma bovis. Vet. Rec. 146, 745747.

    • Search Google Scholar
    • Export Citation
  • Badolato, R., Wang, J. M., Murphy, W. J., Lloyd, A. R., Michiel, D. F., Bausserman, L. L., Kelvin, D. J. and Oppenheim, J. J. (1994): Serum amyloid A is a chemoattractant: induction of migration, adhesion, and tissue infiltration of monocytes and polymorphonuclear leukocytes. J. Exp. Med. 180, 203209.

    • Search Google Scholar
    • Export Citation
  • Barrow, G. I. and Feltham, R. K. A. (1993): Cowan and Steel’s Manual for the Identification of Medical Bacteria. Cambridge University Press, Cambridge.

    • Search Google Scholar
    • Export Citation
  • Ceciliani, F., Ceron, J. J., Eckersall, P. D. and Sauerwein, H. (2012): Acute phase proteins in ruminants. J. Proteomics 75, 42074231.

    • Search Google Scholar
    • Export Citation
  • Dudek, K., Bednarek, D., Ayling, R. D., Kycko, A., Szacawa, E. and Karpińska, T. A. (2016): An experimental vaccine composed of two adjuvants gives protection against Mycoplasma bovis in calves. Vaccine 34, 30513058.

    • Search Google Scholar
    • Export Citation
  • Dudek, K., Bednarek, D., Ayling, R. D. and Szacawa, E. (2013): Immunomodulatory effect of Mycoplasma bovis in experimentally infected calves. Bull. Vet. Inst. Pulawy 57, 499506.

    • Search Google Scholar
    • Export Citation
  • Dudek, K., Bednarek, D. and Szacawa, E. (2011a): Evaluation of immune response in seropositive cattle for Mycoplasma bovis. Bull. Vet. Inst. Pulawy 55, 631634.

    • Search Google Scholar
    • Export Citation
  • Dudek, K., Bednarek, D. and Szacawa, E. (2011b): Stimulating effect of Mycoplasma bovis infection on proinflammatory response in infected cattle. Bull. Vet. Inst. Pulawy 55, 599602.

    • Search Google Scholar
    • Export Citation
  • Dudek, K., Bednarek, D., Szacawa, E., Rosales, R. S. and Ayling, R. D. (2015): Flow cytometry follow-up analysis of peripheral blood leukocyte subpopulations in calves experimentally infected with field isolates of Mycoplasma bovis. Acta Vet. Hung. 63, 167178.

    • Search Google Scholar
    • Export Citation
  • Estes, D. M., Hirano, A., Heussler, V. T., Dobbelaere, D. A. E. and Brown, W. C. (1995): Expression and biological activities of bovine interleukin 4: effects of recombinant bovine interleukin 4 on T cell proliferation and B cell differentiation and proliferation in vitro. Cell. Immunol. 163, 268279.

    • Search Google Scholar
    • Export Citation
  • Fang, F. C. (2004): Antimicrobial reactive oxygen and nitrogen species: concepts and controversies. Nat. Rev. Microbiol. 2, 820832.

  • Farias, M. V. N., Lendez, P. A., Marin, M., Quintana, S., Martínez-Cuesta, L., Ceriani, M. C. and Dolcini, G. L. (2016): Toll-like receptors, IFN-γ and IL-12 expression in bovine leukemia virus-infected animals with low or high proviral load. Res. Vet. Sci. 107, 190195.

    • Search Google Scholar
    • Export Citation
  • Gruys, E., Toussaint, M. J., Niewold, T. A. and Koopmans, S. J. (2005): Acute phase reaction and acute phase proteins. J. Zhejiang Univ. Sci. B. 6, 10451056.

    • Search Google Scholar
    • Export Citation
  • Heinrich, P. C., Castell, J. V. and Andus, T. (1990): Interleukin-6 and the acute phase response. Biochem. J. 265, 621636.

  • Hermeyer, K., Buchenau, I., Thomasmeyer, A., Baum, B., Spergser, J., Rosengarten, R. and Hewicker-Trautwein, M. (2012): Chronic pneumonia in calves after experimental infection with Mycoplasma bovis strain 1067: Characterization of lung pathology, persistence of variable surface protein antigens and local immune response Acta Vet. Scand. 54, 9.

    • Search Google Scholar
    • Export Citation
  • Knolle, P., Löhr, H., Treichel, U., Dienes, H. P., Lohse, A., Schlaack, J. and Gerken, G. (1995): Parenchymal and nonparenchymal liver cells and their interaction in the local immune response. Z. Gastroenterol. 33, 613620.

    • Search Google Scholar
    • Export Citation
  • Linke, R. P., Bock, V., Valet, G. and Rothe, G. (1991): Inhibition of the oxidative burst response of N-formyl peptide-stimulated neutrophils by serum amyloid-A protein. Biochem. Biophys. Res. Commun. 176, 11001105.

    • Search Google Scholar
    • Export Citation
  • Maunsell, F. P., Donovan, G. A., Risco, C. and Brown, M. B. (2009): Field evaluation of a Mycoplasma bovis bacterin in young dairy calves. Vaccine 27, 27812788.

    • Search Google Scholar
    • Export Citation
  • Maunsell, F. P., Woolums, A. R., Francoz, D., Rosenbusch, R. F., Step, D. L., Wilson, D. J. and Janzen, E. D. (2011): Mycoplasma bovis infections in cattle. J. Vet. Intern. Med. 25, 772783.

    • Search Google Scholar
    • Export Citation
  • Nicholas, R. A. J. and Ayling, R. D. (2003): Mycoplasma bovis: disease, diagnosis and control. Res. Vet. Sci. 74, 105112.

  • Nicholas, R. A. J. and Baker, S. E. (1998): Recovery of mycoplasmas from animals. In: Miles, R. J. and Nicholas, R. A. J. (eds) Mycoplasma Protocols. Humana Press, Totowa. pp. 3744.

    • Search Google Scholar
    • Export Citation
  • Nicholas, R. A. J., Ayling, R. D. and Stipkovits, L. P. (2002): An experimental vaccine for calf pneumonia caused by Mycoplasma bovis: clinical, cultural, serological and pathological findings. Vaccine 20, 35693575.

    • Search Google Scholar
    • Export Citation
  • Nicholas, R. A. J., Rosales, R. S. and Loria, G. R. (2017): Mycoplasmology: the big issues. AHDVS 1, 14.

  • O’garra, A. and Vieira, P. (2004): Regulatory T cells and mechanisms of immune system control. Nat. Med. 10, 801805.

  • Oh, S. K., Pavlotsky, N. and Tauber, A. I. (1990): Specific binding of haptoglobin to human neutrophils and its functional consequences J. Leukoc. Biol. 47, 142148.

    • Search Google Scholar
    • Export Citation
  • Postgate, J. R. (1969): Viable counts and viability. In: Norris, J. R. and Ribbons, D. W. (eds) Methods in Microbiology, Academic Press Inc., New York. pp. 611628.

    • Search Google Scholar
    • Export Citation
  • Saraiva, M. and O’Garra, A. (2010): The regulation of IL-10 production by immune cells. Nat. Rev. Immunol. 10, 170181.

  • Schindler, R., Mancilla, J., Endres, S., Ghorbani, R., Clark, S. C. and Dinarello, C. A. (1990): Correlations and interactions in the production of interleukin-6 (IL-6), IL-1, and tumor necrosis factor (TNF) in human blood mononuclear cells: IL-6 suppresses IL-1 and TNF. Blood 75, 4047.

    • Search Google Scholar
    • Export Citation
  • Soehnlen, M. K., Aydin, A., Lengerich, E. J., Houser, B. A., Fenton, G. D., Lysczek, H. R., Burns, C. M., Byler, L. I., Hattel, A. L., Wolfgang, D. R. and Jayarao, B. M. (2011): Blinded, controlled field trial of two commercially available Mycoplasma bovis bacterin vaccines in veal calves. Vaccine 29, 53475354.

    • Search Google Scholar
    • Export Citation
  • Szacawa, E., Niemczuk, K., Dudek, K., Bednarek, D., Rosales, R. and Ayling, R. (2015): Mycoplasma bovis infections and co-infections with other Mycoplasma spp. with different clinical manifestations in affected cattle herds in eastern region of Poland. Bull. Vet. Inst. Pulawy 59, 331337.

    • Search Google Scholar
    • Export Citation
  • Thomas, C. B., Van Ess, P., Wolfgram, L. J., Riebe, J., Sharp, P. and Schultz, R. D. (1991): Adherence to bovine neutrophils and suppression of neutrophil chemiluminescence by Mycoplasma bovis. Vet. Immunol. Immunopathol. 27, 365381.

    • Search Google Scholar
    • Export Citation
  • van Miert, A. S. (1995): Pro-inflammatory cytokines in a ruminant model: pathophysiological, pharmacological, and therapeutic aspects. Vet. Q. 17, 4150.

    • Search Google Scholar
    • Export Citation
  • Vanden Bush, T. J. and Rosenbusch, R. F. (2003): Characterization of the immune response to Mycoplasma bovis lung infection. Vet. Immunol. Immunopathol. 94, 2333.

    • Search Google Scholar
    • Export Citation
  • Zhang, R., Han, X., Chen, Y., Mustafa, R., Qi, J., Chen, X., Hu, C., Chen, H. and Guo, A. (2014): Attenuated Mycoplasma bovis strains provide protection against virulent infection in calves. Vaccine 32, 31073114.

    • Search Google Scholar
    • Export Citation

The author instruction is available in PDF.
Please, download the file from HERE.

 

MANUSCRIPT SUBMISSION

  • Impact Factor (2019): 0.991
  • Scimago Journal Rank (2019): 0.372
  • SJR Hirsch-Index (2019): 32
  • SJR Quartile Score (2019): Q2 Veterinary (miscellaneous)
  • Impact Factor (2018): 1.059
  • Scimago Journal Rank (2018): 0.517
  • SJR Hirsch-Index (2018): 30
  • SJR Quartile Score (2018): Q2 Veterinary (miscellaneous)

Language: English

Founded in 1951
Publication: One volume of four issues annually
Publication Programme: 2020. Vol. 68.
Indexing and Abstracting Services:

  • Biological Abstracts

     

  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Subscribers can access the electronic version of every printed article.

Senior editors

Editor(s)-in-Chief: Benkő, Mária

Managing Editor(s): Székely, András

Editorial Board

      Dénes, Béla
      Eszterbauer, Edit
      Fébel, Hedvig
      Fodor, László
      Harrach, Balázs
      Andras Komaromy (USA)
      Peter Massanyi (Slovak Republic)
      Nagy, Béla
      Németh, Tibor
      Neogrády, Zsuzsanna
      Kurt Pfister (Germany)
      Solti, László
      Szabó, József
      Vajdovich, Péter
      Varga, János
      Štefan, Vilĉek (Slovak Republic)
      Vörös, Károly
      Herbert Weissenböck (Austria)
      Zsarnovszky, Attila

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793