View More View Less
  • 1 Research Institute for Animal Breeding, Nutrition and Meat Science, Gesztenyés u. 1, H-2053 Herceghalom, Hungary
  • 2 Szent István University, Faculty of Agricultural And Environmental Sciences, Gödöllő, Hungary
  • 3 BioTracking, Inc., Moscow, Idaho, USA
Full access

The early detection of pregnancy and the determination of fetal numbers have economic benefits in sheep production because of the seasonal breeding patterns where missing a breeding opportunity means the loss of one productive year. The purpose of this study was to evaluate the efficacy of the B6-HRP ELISA for ovine pregnancy-specific protein B (oPSPB) measurement in the detection of pregnancy and estimation of fetal numbers in different sheep breeds. BioPRYN® ELISA assay kit was used for the detection of pregnancy in the experimental animals. Ninety-three ewes of three breeds (British Milksheep – BM, Lacaune – L and Transylvanian Racka – TR), each from three farms in Hungary, were included in the study. BM and L ewes were artificially inseminated (AI). Thirty-five days after AI, all ewes were examined by transabdominal ultrasound. The TR flock was mated naturally over a six-week period. At the end of the mating period, the ewes were similarly examined by ultrasound. Blood samples were taken from all pregnant ewes twice (35 and 65 days after AI), and serum samples were assayed by the BioPRYN test. It can be concluded that the detection of serum PSPB by ELISA is a much easier, safer, less expensive and highly accurate method for the detection of ovine pregnancy. Although some breed-related differences were detectable at 35 and 65 days post breeding, no differences in oPSPB levels were found in pregnant ewes carrying different numbers of fetuses.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • Butler, J. E., Hamilton, W. C., Sasser, R. G., Ruder, C. A., Hass, G. M. and Williams, R. R. (1982): Detection and partial characterization of two bovine pregnancy-specific proteins. Biol. Reprod. 26, 925933.

    • Search Google Scholar
    • Export Citation
  • Camous, S., Charpigny, G., Guillomot, M., Martal, J. and Sasser, R. G. (1988): Purification of one pregnancy-specific protein by high-performance liquid chromatography (HPLC). Proc. Bard Workshop, Maternal Recognition of Pregnancy and Maintenance of the Corpus Luteum. Abstract 2, Jerusalem, 20–24 March 1988.

    • Search Google Scholar
    • Export Citation
  • Del Vecchio, R. P. D., Sutherland, W. D. and Sasser, R. G. (1995): Effect of pregnancy-specific protein B on luteal cell progesterone, prostaglandin, and oxytocin production during two stages of the bovine estrus cycle. J. Anim. Sci. 73, 26622668.

    • Search Google Scholar
    • Export Citation
  • Eckblad, W. P., Sasser, R. G., Ruder, C. A., Panlasigui, P. and Kuczynski, T. (1985): Localization of pregnancy-specific protein B (PSPB) in bovine placental cells using glucose oxidase– anti-glucose oxidase immunohistochemical stain. J. Anim. Sci. 61 (Suppl.), 149150.

    • Search Google Scholar
    • Export Citation
  • Gonzalez, F., Sulon, J., Calero, P., Batista, M., Gracia, A. and Beckers, J. F. (2001): Pregnancyassociated glycoproteins (PAG) detection in milk samples for pregnancy diagnosis in dairy goats. Theriogenology 56, 671676.

    • Search Google Scholar
    • Export Citation
  • Gonzalez, F., Sulon, J., Garbayo, J. M., Batista, M., Cabrera, F., Calero, P., Gracia, A. and Beckers, J. F. (1999): Early pregnancy diagnosis in goats by determination of pregnancyassociated glycoprotein concentrations in plasma samples. Theriogenology 52, 717725.

    • Search Google Scholar
    • Export Citation
  • Howard, J., Gabor, G., Gray, T., Passavant, C., Ahmadzadeh, A., Sasser, N., Pals, D. and Sasser, R. G. (2007): BioPRYN – a blood-based pregnancy test for managing breeding and pregnancy in cattle. Proc. West. Sect. Am. Soc. Anim. Sci. 58, 295298.

    • Search Google Scholar
    • Export Citation
  • Humblot, P., De Montigny, G., Jeanguyot, N., Tetedoie, F., Payen, B., Thibier, M. and Sasser, R. G. (1990): Pregnancy-specific protein B and progesterone concentrations in French Alpine Goats throughout gestation. J. Reprod. Fertil. 89, 205212.

    • Search Google Scholar
    • Export Citation
  • Karen, A., Beckers, J. F., Sulon, J., Sousa, N. M., Szabados, K., Reiczigel, J. and Szenci, O. (2003): Early pregnancy diagnosis in sheep by progesterone and pregnancy-associated glycoprotein tests. Theriogenology 59, 19411948.

    • Search Google Scholar
    • Export Citation
  • Lee, C. S., Wooding, F. B. P. and Brandon, M. R. (1986): Immunogold co-localisation of ovine placental lactogen and the antibody recognized by the SBU-3 monoclonal antibody in sheep placental granules. J. Reprod. Fertil. 78, 653662.

    • Search Google Scholar
    • Export Citation
  • Paré, J., Audet-Grenier, M-H., Rouillier, P. and Sirard, M-A. (2008): Evaluation of the DG29 test for early detection of pregnancy in cattle. Can. Vet. J. 49, 11191121.

    • Search Google Scholar
    • Export Citation
  • Ranilla, M. J., Sulon, J., Carro, M. D., Mantecon, A. R. and Beckers, J. F. (1994): Plasmatic profiles of pregnancy-associated glycoprotein and progesterone levels during gestation in Churra and Merino sheep. Theriogenology 42, 537545.

    • Search Google Scholar
    • Export Citation
  • Reimers, T. J., Sasser, R. G. and Ruder, C. A. (1985): Production of pregnancy-specific protein by bovine binucleate trophoblastic cells. Biol. Reprod. 32 (Suppl. 1), Abstract 55.

    • Search Google Scholar
    • Export Citation
  • Ruder, C. A., Stellflug, J. N., Dahmen, J. J. and Sasser, R. G. (1988): Detection of pregnancy in sheep by radioimmunoassay of sera for pregnancy-specific protein B. Theriogenology 29, 905912.

    • Search Google Scholar
    • Export Citation
  • Sasser, R. G., Ruder, C. A., Ivani, K. A., Butler, J. E. and Hamilton, W. C. (1986): Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biol. Reprod. 35, 396.

    • Search Google Scholar
    • Export Citation
  • Sasser, R. G., Williams, R. J., Bull, R. C., Ruder, C. A. and Falk, D. G. (1988): Postpartum reproductive performance in crude protein-restricted beef cows: return to estrus and conception. J. Anim. Sci. 66, 30333039.

    • Search Google Scholar
    • Export Citation
  • Vasques, M. I., Horta, A. E. M., Marques, C. C., Sasser, R. G. and Humblot, P. (1995): Levels of bPSPB throughout single and twin pregnancies after AI or transfer of IVM/IVF cattle embryos. Anim. Reprod. Sci. 38, 279289.

    • Search Google Scholar
    • Export Citation
  • Wallace, J. M., Aitken, R. P., Cheyne, M. A. and Humblot, P. (1997): Pregnancy-specific protein B and progesterone concentrations in relation to nutritional regimen, placental mass and pregnancy outcome in growing adolescent ewes carrying singleton fetuses. J. Reprod. Fertil. 109, 5358.

    • Search Google Scholar
    • Export Citation
  • Willard, J. M., Ruder, C. A., Sasser, R. G. and Stellflug, J. (1987): Ovine pregnancy-specific protein B concentration in the sera of early pregnant and peripartum ewes. J. Anim. Sci. 65 (Suppl.), 514.

    • Search Google Scholar
    • Export Citation
  • Willard, J. M., White, D. R., Wesson, C. A., Stellflug, J. and Sasser, R. G. (1995): Detection of fetal twins in sheep using a radioimmunoassay for pregnancy-specific protein B. J. Anim. Sci. 73, 960966.

    • Search Google Scholar
    • Export Citation
  • Zoli, A. P., Guilbault, L. A., Delahaut, P., Ortiz, W. B. and Beckers, J. (1992): Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol. Reprod. 46, 8392.

    • Search Google Scholar
    • Export Citation

  • Impact Factor (2018): 1.059
  • Veterinary (miscellaneous) SJR Quartile Score (2018): Q2
  • Scimago Journal Rank (2018): 0.517
  • SJR Hirsch-Index (2018): 30

Language: English

Founded in 1951
Publication: One volume of four issues annually
Publication Programme: 2020. Vol. 68.
Indexing and Abstracting Services:

  • Biological Abstracts

     

  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture, Biology and Environmental Sciences
  • Elsevier Science Navigator
  • Focus On: Veterinary Science and Medicine
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Subscribers can access the electronic version of every printed article.

Senior editors

Editor(s)-in-Chief: Benkő, Mária

Managing Editor(s): Székely, András

Editorial Board

      Dénes, Béla
      Eszterbauer, Edit
      Fébel, Hedvig
      Fodor, László
      Harrach, Balázs
      Andras Komaromy (USA)
      Peter Massanyi (Slovak Republic)
      Nagy, Béla
      Németh, Tibor
      Neogrády, Zsuzsanna
      Kurt Pfister (Germany)
      Solti, László
      Szabó, József
      Vajdovich, Péter
      Varga, János
      Štefan, Vilĉek (Slovak Republic)
      Vörös, Károly
      Herbert Weissenböck (Austria)
      Zsarnovszky, Attila

ACTA VETERINARIA HUNGARICA
Institute for Veterinary Medical Research
Centre for Agricultural Research
Hungarian Academy of Sciences
P.O. Box 18, H-1581 Budapest, Hungary
Phone: (36 1) 467 4081 (ed.-in-chief) or (36 1) 213 9793 (editor) Fax: (36 1) 467 4076 (ed.-in-chief) or (36 1) 213 9793

The author instruction is available in PDF.
Please, download the file from HERE.

 

MANUSCRIPT SUBMISSION